Broad spectrum anti-biotic activity and disease suppression by the potential biocontrol agent Burkholderia ambifaria BC-F

Li, W; Roberts, Daniel P.; Déry, Pierre; Meyer, Seth D.; Lohrke, Scott M.; Lumsden, R. D.; Hebbar, K.P.

doi:10.1016/s0261-2194(01)00074-6

Cited by 57 publications

(35 citation statements)

References 32 publications

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“…ambifaria BC-F, S. marcescens isolates N1-6, N1-14, and N2-4 and T. virens isolates GL3 and GL21 had broad-spectrum activity against soilborne pathogens of cucumber. In addition to suppressing damping-off caused by the fungal pathogens R. solani and/or P. ultimum, culture filtrates from BC-F, N1-6, N1-14, N2-4, and GL3 and GL21 inhibited in vitro egg hatch by the nematode M. incognita in experiments reported here or elsewhere (Meyer et al, 2000;Li et al, 2002). This broad-spectrum activity is likely due, in part, to inhibitory metabolites produced by these organisms.…”

Section: Discussionmentioning

confidence: 57%

Biocontrol agents applied individually and in combination for suppression of soilborne diseases of cucumber

et al. 2005

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Lewis, Jack A.; and Chung, Soohee, "Biocontrol agents applied individually and in combination for suppression of soilborne diseases of cucumber" (2005 AbstractThe soilborne pathogens Rhizoctonia solani, Pythium ultimum, and Meloidogyne incognita can cause severe economic losses to field-and greenhouse-grown cucumber. A collection of bacterial isolates and isolates GL3 and GL21 of Trichoderma virens were screened for suppression of diseases caused by these pathogens. T. virens isolates GL3 and GL21 provided the most effective suppression of damping-off caused by R. solani in greenhouse bioassays. Burkholderia ambifaria BC-F, B. cepacia BC-1, and Serratia marcescens N1-14 also provided significant suppression of R. solani relative to the pathogen check in some experiments. T. virens isolates GL3 and GL21 and S. marcescens isolates N1-6, N1-14, and N2-4 provided the most consistent and effective suppression of damping-off of cucumber caused by P. ultimum in growth chamber experiments. No microbial treatment containing individual or combined microbes significantly suppressed populations of M. incognita on cucumber or improved plant vigor in greenhouse bioassays. T. virens GL21 applied as a granular formulation, in combination with B. cepacia BC-1 or B. ambifaria BC-F applied as a seed treatment, significantly improved suppression of damping-off caused by R. solani over individual applications of these microbes in at least one experiment. Treatments combining B. cepacia BC-1, B. ambifaria BC-F, or S. marcescens isolates N1-14 or N2-4 with T. virens GL21 in R. solani biocontrol assays always resulted in plant stands that were similar or greater than treatments containing individual applications of these microbes. B. ambifaria BC-F combined with T. virens GL21 in seed treatments resulted in significantly improved suppression of damping-off caused by P. ultimum in two of three experiments. Populations of T. virens GL3 and GL21 were both substantially reduced after coincubation with B. cepacia BC-1, or S. marcescens isolates N1-14 or N2-4 for 10 to 12 d in cucumber rhizospheres. Populations of T. virens GL21 were slightly reduced after coincubation with B. ambifaria BC-F. Results presented here substantiate other studies reporting enhanced biocontrol performance This article is a U.S. government work, and is not subject to copyright in the United States.with certain combinations of biocontrol agents. These results also indicate that antagonism among combinations of biocontrol agents can vary with the assay system employed. r

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Section: Discussionmentioning

confidence: 57%

Biocontrol agents applied individually and in combination for suppression of soilborne diseases of cucumber

et al. 2005

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“…Dual culture has been used to understand antagonism between different biological agents (Li et al 2002;Anith et al 2003;Aravind et al 2009). The endophytic root symbiont P. indica induced various reactions with rhizobacterial strains.…”

Section: Discussionmentioning

confidence: 99%

The growth of tomato seedlings inoculated with co-cultivated Piriformospora indica and Bacillus pumilus

Anith

Sreekumar

2015

Symbiosis

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A novel co-culture method using the beneficial root endophytic fungus Piriformospora indica and a plant-growth promoting rhizobacterial strain of Bacillus pumilus to promote the growth of tomato seedlings in plug trays, is described. Coconut water, a waste product from the coconut industry, was used as medium for co-cultivation of the two biological agents. During the co-culture with the fungus, the bacterial strain showed a similar growth rate to that in monoculture in fresh autoclaved coconut water. In contrast, co-culture in potato dextrose broth (PDB), a medium routinely used for cultivation of the endophytic fungus, did not support bacterial growth. Inoculation with the co-culture or a mixture of P. indica and B. pumilus promoted tomato seedling growth significantly when compared with individual application of the two biological agents. No difference was observed with respect to the degree of root colonization in tomato seedlings whether it was done with a monoculture or a co-culture with rhizobacterium. The co-culture system developed reduces the cost of inoculum production as it uses coconut water, a cheap and locally available waste product and a single fermentation vessel. Further research is required to know the endophytic nature of the rhizobacterial strain and its possible role in helping the fungus in root colonization.

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“…Interestingly, Burkholderia ambifaria, which can use NFL as a sole carbon and nitrogen source (Roberts and Lindow 2014) is often found associated with a variety of plant roots, and is a dominant colonizer of tall grass prairies. Burkholderia ambifaria has also been shown to produce compounds that promote plant growth and protect the grass host from fungal pathogens (Coenye and Vandamme 2003;Li et al 2002;Mahenthiralingam et al 2011). It is thought that the Epichloë endophyte of F. arundinacea acts as a selective agent in recruitment of beneficial rhizosphere microbes and that loline alkaloids are one resource that enriches for such bacteria.…”

Section: Introductionmentioning

confidence: 99%

Rhizosphere microbiome selection by Epichloë endophytes of Festuca arundinacea

Roberts

Ferraro

2015

Plant Soil

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Aims Loline alkaloids produced by Epichloë spp. are known to deter feeding by insect herbivores while also serving as a significant carbon source for certain epiphytic bacteria on tall fescue leaves. In this study we examined the role of loline alkaloids in attracting certain bacteria to the rhizosphere of tall fescue plants that harbor loline producing fungal endophytes. Methods Population studies were used to compare the fitness of known loline catabolizing strains to other rhizosphere bacteria. Pyrosequencing of 16S rRNA fragments compared the composition of bacterial communities inhabiting the endophyte infected tall fescue (Festuca arundinacea) rhizosphere to those of endophyte free fescue plants.Results Rhizosphere population studies demonstrated that loline catabolizing strains Burkholderia ambifaria 7R and Pseudomonas aureofaciens outcompete and suppress the growth of non-loline catabolizing strains. Pyrosequencing of 16S rRNA fragments showed greater percentages of certain plant growth promoting bacteria in rhizosperes seeded with B. ambifaria 7R than noninoculated soils. Rhizospheres of endophyte infected plants showed higher species richness (Shannon diversity index=4.03) over endophyte free rhizospheres (Shannon diversity index=3.08) and a greater percentage of Firmicutes. Conclusions The differences in microbial community composition between endophyte-infected and endophytefree rhizospheres suggest that the presence of fungal endophytes influences microbial community structure. Loline alkaloid production may be one proxy by which the fungal endophyte shapes microbial communities, as evidenced by increased fitness of loline catabolizing bacteria in the tall fescue rhizosphere.

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Broad spectrum anti-biotic activity and disease suppression by the potential biocontrol agent Burkholderia ambifaria BC-F

Cited by 57 publications

References 32 publications

Biocontrol agents applied individually and in combination for suppression of soilborne diseases of cucumber

Biocontrol agents applied individually and in combination for suppression of soilborne diseases of cucumber

The growth of tomato seedlings inoculated with co-cultivated Piriformospora indica and Bacillus pumilus

Rhizosphere microbiome selection by Epichloë endophytes of Festuca arundinacea

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