Broad-range antagonistic rhizobacteria Pseudomonas fluorescens and Serratia plymuthica suppress Agrobacterium crown gall tumours on tomato plants

Dandurishvili, N.; Toklikishvili, Natela; Ovadis, Marianna; Eliashvili, P.; Giorgobiani, N.; Keshelava, R.; Тедиашвили, Марина; Vainstein, Alexander; Khmel, I. A.; Szegedi, E.; Chernin, Leonid

doi:10.1111/j.1365-2672.2010.04891.x

Cited by 155 publications

(132 citation statements)

References 32 publications

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“…In a meta-analysis of 31 studies on volatiles emitted by six common pathogenic bacteria, 1-undecene was one of the molecules that differentiated Pseudomonas aeruginosa from the other, non-Pseudomonas pathogenic bacteria (32). Within the genus Pseudomonas, it seems that the relative contribution of undecene to the volatile bouquet of the strains is highly variable, ranging from the unchallenged dominance observed in our rhizosphere strains or in other antagonistic P. fluorescens strains (33) to the absence of detection for some strains, such as P. syringae S22 and P. jessenii S34. Other studies have reported 1-undecene from the headspace of Pseudomonas strains (7,(34)(35)(36) but also from other species, including members of the genera Burkholderia, Bacillus, and Serratia (7, 37).…”

Section: Discussionmentioning

confidence: 99%

Pseudomonas Strains Naturally Associated with Potato Plants Produce Volatiles with High Potential for Inhibition of Phytophthora infestans

Hunziker

Bönisch

Groenhagen

et al. 2015

Appl Environ Microbiol

184

140

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bBacteria emit volatile organic compounds with a wide range of effects on bacteria, fungi, plants, and animals. The antifungal potential of bacterial volatiles has been investigated with a broad span of phytopathogenic organisms, yet the reaction of oomycetes to these volatile signals is largely unknown. For instance, the response of the late blight-causing agent and most devastating oomycete pathogen worldwide, Phytophthora infestans, to bacterial volatiles has not been assessed so far. In this work, we analyzed this response and compared it to that of selected fungal and bacterial potato pathogens, using newly isolated, potato-associated bacterial strains as volatile emitters. P. infestans was highly susceptible to bacterial volatiles, while fungal and bacterial pathogens were less sensitive. Cyanogenic Pseudomonas strains were the most active, leading to complete growth inhibition, yet noncyanogenic ones also produced antioomycete volatiles. Headspace analysis of the emitted volatiles revealed 1-undecene as a compound produced by strains inducing volatile-mediated P. infestans growth inhibition. Supplying pure 1-undecene to P. infestans significantly reduced mycelial growth, sporangium formation, germination, and zoospore release in a dose-dependent manner. This work demonstrates the high sensitivity of P. infestans to bacterial volatiles and opens new perspectives for sustainable control of this devastating pathogen. During the last decade, it has become evident that bacteria communicate with other organisms through the emission of volatile compounds. Highly significant volatile-mediated effects of bacteria have been reported for various target organisms, including bacteria themselves (1-5), plants (5-9), and fungi (10-12). The research carried out to understand the nature of this volatile-mediated interaction of bacteria with plants and with other bacteria has focused so far on model organisms (e.g., Arabidopsis thaliana and Escherichia coli) and has enabled identification of some of the active compounds involved in the respective interactions, such as indole, 2,3-butanediol, dimethyl disulfide, hydrogen sulfide, and ammonia. The research on model organisms has also contributed to understanding of the mechanisms underlying the observed phenotypic changes of increased (13-15) or decreased (16, 17) plant biomass and increased antibiotic resistance in bacteria (2-4, 18).As far as fungi are concerned, most studies investigating their response to bacterial volatiles have focused on potential application and have thus largely neglected deeper investigation of the chemical nature of the active compounds and/or of the mode of action of these molecules. In addition to the inorganic volatiles hydrogen cyanide (19) and ammonia (20), few volatile organic compounds, such as sulfur compounds and long-chain ketones, have been unequivocally shown to inhibit the growth of phytopathogenic fungi when applied at biologically relevant concentrations (12). With the ultimate prospect of using the antifungal potential of bacterial...

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Section: Discussionmentioning

confidence: 99%

Pseudomonas Strains Naturally Associated with Potato Plants Produce Volatiles with High Potential for Inhibition of Phytophthora infestans

Hunziker

Bönisch

Groenhagen

et al. 2015

Appl Environ Microbiol

184

140

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“…DMDS was first described as produced by bacteria isolated from decomposing chicken (Freeman et al, 1976). Surprisingly, DMDS, along with other VOCs emitted by rhizobacteria Pseudomonas fluorescens B-4117 and Serratia plymuthica IC1270, was found to exert antimicrobial and nematicidal activity Wang et al, 2009;Huang et al, 2010;Dandurishvili et al, 2011) and to inhibit the cell-cell communication quorumsensing network of various bacteria . Furthermore, DMDS functions as a plant defense compound against nonspecialist herbivores feeding on Allium porrum (Dugravot et al, 2003(Dugravot et al, , 2004 and as both an oviposition repellent and an attractant to different natural enemies of the cabbage root fly Delia radicum (Ferry et al, 2007(Ferry et al, , 2009.…”

Section: Discussionmentioning

confidence: 99%

Dimethyl Disulfide Produced by the Naturally Associated Bacterium Bacillus sp B55 Promotes Nicotiana attenuata Growth by Enhancing Sulfur Nutrition

et al. 2013

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Bacillus sp B55, a bacterium naturally associated with Nicotiana attenuata roots, promotes growth and survival of wild-type and, particularly, ethylene (ET)-insensitive 35S-ethylene response1 (etr1) N. attenuata plants, which heterologously express the mutant Arabidopsis thaliana receptor ETR1-1. We found that the volatile organic compound (VOC) blend emitted by B55 promotes seedling growth, which is dominated by the S-containing compound dimethyl disulfide (DMDS). DMDS was depleted from the headspace during cocultivation with seedlings in bipartite Petri dishes, and 35 S was assimilated from the bacterial VOC bouquet and incorporated into plant proteins. In wild-type and 35S-etr1 seedlings grown under different sulfate (SO 4 22 ) supply conditions, exposure to synthetic DMDS led to genotype-dependent plant growth promotion effects. For the wild type, only S-starved seedlings benefited from DMDS exposure. By contrast, growth of 35S-etr1 seedlings, which we demonstrate to have an unregulated S metabolism, increased at all SO 4 22 supply rates. Exposure to B55 VOCs and DMDS rescued many of the growth phenotypes exhibited by ET-insensitive plants, including the lack of root hairs, poor lateral root growth, and low chlorophyll content. DMDS supplementation significantly reduced the expression of S assimilation genes, as well as Met biosynthesis and recycling. We conclude that DMDS by B55 production is a plant growth promotion mechanism that likely enhances the availability of reduced S, which is particularly beneficial for wild-type plants growing in S-deficient soils and for 35S-etr1 plants due to their impaired S uptake/assimilation/metabolism.

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“…Recently, Dandurishvili et al (2011) reported that volatiles emitted by Pseudomonas fluorescens and Serratia plymuthica have bacteriostatic effects against the bacterial plant pathogens Agrobacterium tumefaciens and A. vitis and inhibited the growth of these pathogens in planta. The major volatile emitted by S. plymuthica under the tested conditions was dimethyl disulphide, whereas P. fluorescens emitted a mix of 1-undecene, methanthiol, methanthiol acetate and dimethyl disulphide (Dandurishvili et al, 2011).…”

Section: Volatile-mediated Antibacterial Activitymentioning

confidence: 99%

Volatile affairs in microbial interactions

et al. 2015

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Microorganisms are important factors in shaping our environment. One key characteristic that has been neglected for a long time is the ability of microorganisms to release chemically diverse volatile compounds. At present, it is clear that the blend of volatiles released by microorganisms can be very complex and often includes many unknown compounds for which the chemical structures remain to be elucidated. The biggest challenge now is to unravel the biological and ecological functions of these microbial volatiles. There is increasing evidence that microbial volatiles can act as infochemicals in interactions among microbes and between microbes and their eukaryotic hosts. Here, we review and discuss recent advances in understanding the natural roles of volatiles in microbe-microbe interactions. Specific emphasis will be given to the antimicrobial activities of microbial volatiles and their effects on bacterial quorum sensing, motility, gene expression and antibiotic resistance.

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Broad-range antagonistic rhizobacteria Pseudomonas fluorescens and Serratia plymuthica suppress Agrobacterium crown gall tumours on tomato plants

Cited by 155 publications

References 32 publications

Pseudomonas Strains Naturally Associated with Potato Plants Produce Volatiles with High Potential for Inhibition of Phytophthora infestans

Pseudomonas Strains Naturally Associated with Potato Plants Produce Volatiles with High Potential for Inhibition of Phytophthora infestans

Dimethyl Disulfide Produced by the Naturally Associated Bacterium Bacillus sp B55 Promotes Nicotiana attenuata Growth by Enhancing Sulfur Nutrition

Volatile affairs in microbial interactions

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