Broad host range vectors derived from an RSF1010::Tn1 plasmid

Chistoserdov, A. Y.; Tsygankov, Y. D.

doi:10.1016/0147-619x(86)90053-3

Cited by 21 publications

(7 citation statements)

References 12 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Initially, a DNA fragment containing the genes from E. coli plasmid, pMH10 (Akhverdyan et al 2007), for the thermo-induced transposition factors was cloned into vectors containing the broad host range replicons of the IncQ (Chistoserdov and Tsygankov 1986) or IncPα groups (Ditta et al 1985; Pansegrau et al 1994). These new (E-plus)-helpers (Fig.…”

Section: Adaptation Of the Mu-driven System For Methylophilus Methylomentioning

confidence: 99%

Application of the bacteriophage Mu-driven system for the integration/amplification of target genes in the chromosomes of engineered Gram-negative bacteria—mini review

Akhverdyan

Gak

Tokmakova

et al. 2011

Appl Microbiol Biotechnol

View full text Add to dashboard Cite

The advantages of phage Mu transposition-based systems for the chromosomal editing of plasmid-less strains are reviewed. The cis and trans requirements for Mu phage-mediated transposition, which include the L/R ends of the Mu DNA, the transposition factors MuA and MuB, and the cis/trans functioning of the E element as an enhancer, are presented. Mini-Mu(LR)/(LER) units are Mu derivatives that lack most of the Mu genes but contain the L/R ends or a properly arranged E element in cis to the L/R ends. The dual-component system, which consists of an integrative plasmid with a mini-Mu and an easily eliminated helper plasmid encoding inducible transposition factors, is described in detail as a tool for the integration/amplification of recombinant DNAs. This chromosomal editing method is based on replicative transposition through the formation of a cointegrate that can be resolved in a recombination-dependent manner. (E-plus)- or (E-minus)-helpers that differ in the presence of the trans-acting E element are used to achieve the proper mini-Mu transposition intensity. The systems that have been developed for the construction of stably maintained mini-Mu multi-integrant strains of Escherichia coli and Methylophilus methylotrophus are described. A novel integration/amplification/fixation strategy is proposed for consecutive independent replicative transpositions of different mini-Mu(LER) units with “excisable” E elements in methylotrophic cells.

show abstract

Section: Adaptation Of the Mu-driven System For Methylophilus Methylomentioning

confidence: 99%

Application of the bacteriophage Mu-driven system for the integration/amplification of target genes in the chromosomes of engineered Gram-negative bacteria—mini review

Akhverdyan

Gak

Tokmakova

et al. 2011

Appl Microbiol Biotechnol

View full text Add to dashboard Cite

show abstract

“…Numerous broad-host-range shuttle vectors, promoter-probe vectors, expression vectors and other special-purpose vectors are available now. The majority of them are based on the replicons of the IncQ or IncP groups [see for example [ 1 - 3 ]].…”

Section: Introductionmentioning

confidence: 99%

Construction of stably maintained non-mobilizable derivatives of RSF1010 lacking all known elements essential for mobilization

et al. 2007

View full text Add to dashboard Cite

Background: RSF1010 is a well-studied broad-host-range plasmid able to be mobilized to different bacteria and plants. RSF1010-derived plasmid vectors are widely used in both basic research and industrial applications. In the latter case, exploiting of mobilizable plasmids or even the plasmids possessing negligible mobilization frequency, but containing DNA fragments that could promote conjugal transfer, is undesirable because of biosafety considerations. Previously, several mutations significantly decreasing efficiency of RSF1010 mobilization have been selected. Nevertheless, construction of the RSF1010 derivative lacking all known loci involved in the conjugal transfer has not been reported yet.

show abstract

“…Construction of recombinant plasmids pAET7 (Ap R , Sm R ) was obtained on the basis of pAYC32 (Chistorerdov and Tsygankov 1986) by cloning of BamHI fragment from pUC-MuAB (Akhverdyan et al 2007). pTP310 (Tc R ) was a result of cloning the same BamHI fragment of pUC-MuAB in broad-host-range replicon pRK310, a derivative of plasmid RP4/RK2 of IncPα group (Ditta et al 1985;Pansegrau et al 1994).…”

Section: Standard Gene Engineering Methodsmentioning

confidence: 99%

Phage Mu-driven two-plasmid system for integration of recombinant DNA in the Methylophilus methylotrophus genome

Abalakina

Tokmakova

Gorshkova

et al. 2008

Appl Microbiol Biotechnol

View full text Add to dashboard Cite

A phage Mu-driven two-plasmid system for DNA integration in Escherichia coli genome has been adjusted for Methylophilus methylotrophus. Constructed helper plasmids with broad-host-range replicons carry thermo-inducible genes for transposition factors MuA and MuB. Integrative plasmids that are only replicated in E. coli could be mobilized to M. methylotrophus and contained mini-Mu unit with a short terminus of Mu DNA, Mu-attL/ R. Mini-Mu unit was integrated in the M. methylotrophus genome via mobilization of the integrative plasmid to the cells carrying the helper in conditions of thermo-induced expression of MuA and MuB. In this system, mini-Mu unit was mainly integrated due to replicative transposition, and the integrated copy could be amplified in the M. methylotrophus chromosome in the presence of helper plasmid. A kan-gene flanked by FRT sites was inserted in one of the mini-Mu units, and it could be readily excised by yeast FLP recombinase that is encoded by the designed plasmid. The multiple Mu-driven gene insertion was carried out by integration of the Bacillus amyloliquefaciens α-amylase gene followed by curing the Km R marker before integration of the second mini-Mu unit with Pseudomonas putida xylE gene encoding catechol 2,3-dioxygenase (C23O).

show abstract

Broad host range vectors derived from an RSF1010::Tn1 plasmid

Cited by 21 publications

References 12 publications

Application of the bacteriophage Mu-driven system for the integration/amplification of target genes in the chromosomes of engineered Gram-negative bacteria—mini review

Application of the bacteriophage Mu-driven system for the integration/amplification of target genes in the chromosomes of engineered Gram-negative bacteria—mini review

Construction of stably maintained non-mobilizable derivatives of RSF1010 lacking all known elements essential for mobilization

Phage Mu-driven two-plasmid system for integration of recombinant DNA in the Methylophilus methylotrophus genome

Contact Info

Product

Resources

About