Broad bean mottle virus: Identification, host range, serology, and occurrence on faba bean (Vicia faba) in West Asia and North Africa

Makkouk, K. M.; Bos, L.; Rizkallah, Alain; Azzam, O.; Katul, L.

doi:10.1007/bf02006546

Cited by 20 publications

(28 citation statements)

References 13 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Although a large number of legume weeds were found susceptible to mechanical inoculation and were thus reported as potential hosts of BBMV (Makkouk et al, 1988), none of the ones tested in the present study were found to host the virus. More sampling and testing is needed, however.…”

Section: Discussionmentioning

confidence: 88%

“…In contrast, common bean and the food legumes pea, chickpea, and lentil were found naturally infected by BBMV. The virus was suspected to occur naturally in food legumes other than faba bean since it has already been found pathogenic to these food legume species and several other fodder and weed legumes upon mechanical inoculation (Makkouk et al, 1988;Fortass and Bos, 1992). Moreover, some isolates of BBMV were found to be more pathogenic on chickpea, lentil, or pea, than on faba bean (Fortass and Bos, 1992), implying therefore that BBMV should be regarded as a foodlegume virus rather than a mere faba-bean virus.…”

Section: Discussionmentioning

confidence: 97%

“…The assay plants used in the transmission experiments were tested for BBMV in double-antibody sandwich ELISA as by Clark and Adams (1977). The antiserum used had been raised against the Moroccan isolate MV90-85 (Makkouk et al, 1988). The g-globulins were used at 1 p~g/ml and the alkaline phosphatase conjugated antibodies were diluted to 1:1000 in the extraction buffer (0.03 M potassium phosphate buffer, pH 7.7).…”

Section: Methodsmentioning

confidence: 99%

“…Another ecological feature suggesting the potential importance of the virus is its wide host range among legume species (Makkouk et al, 1988). Recently, some promising breeding lines of faba bean, chickpea, pea, and lentil were tested and found susceptible and sensitive to the virus (Fortass and Bos, 1992).…”

Section: Sitona Lineatus Var Viridifrons Motsch Was Reported In Portmentioning

confidence: 99%

See 3 more Smart Citations

Broad bean mottle virus in Morocco; curculionid vectors, and natural occurrence in food legumes other than faba bean (Vicia faba)

Fortass

Diallo

1993

Netherlands Journal of Plant Pathology

View full text Add to dashboard Cite

Broad bean mottle virus (BBMV) was u'ansmitted from infected to healthy faha-bean plants by the curculionid weevils Apion radiolus Kirby, Hypera variabilis Herbst, Pachytychius strumarius Gyll, Smicronyx cyaneus Gyll, and Sitona lineatus L. The latter appeared to be an efficient vector: acquisition and inoculation occurred at the first bite, the rate of transmission was c. 41%, and virus retention lasted for at least seven days. S. lineatus transmitted the virus from faba bean to lentil and pea, but not to the three genotypes of chickpea tested. This is the first report on the genera Hypera, Pachytychius, and Smicronyx as virus vectors, and on A. radiolus, H. variabilis, P. strumarius, and S. cyaneus as vectors of BBMV.Out of 351 samples of food legumes with symptoms suggestive of virus infection, 16, 1 l, 19, and 17% of the samples of chickpea, lentil, pea, and common bean, respectively, were found infected when tested for BBMV in DAS-ELISA. This is the first report on the natural occurrence of BBMV in chickpea, lentil, pea, and common bean. The virus should be regarded as a food-legume virus rather than a faba-bean virus solely, and is considered an actual threat to food legume improvement programmes.

show abstract

Section: Discussionmentioning

confidence: 88%

Section: Discussionmentioning

confidence: 97%

Section: Methodsmentioning

confidence: 99%

Section: Sitona Lineatus Var Viridifrons Motsch Was Reported In Portmentioning

confidence: 99%

See 2 more Smart Citations

Broad bean mottle virus in Morocco; curculionid vectors, and natural occurrence in food legumes other than faba bean (Vicia faba)

Fortass

Diallo

1993

Netherlands Journal of Plant Pathology

View full text Add to dashboard Cite

show abstract

“…RNA 1 (3.1 kb), RNA 2 (2-8 kb) and RNA 3 (2.3 kb) of BBMV are each capped and, unlike other bromovirus RNAs, each has an A residue at the 5' terminus (Dzianott & Bujarski, 1991 ;Romero et al, 1992). Several BBMV strains, including those from England [Bawden (Ba)], Morocco (Mo), Sudan (Su), Syria (Suv) and Tunisia (Tu) have been described (Bawden et al, 1951 ;Makkouk et al, 1988). These strains are serologically indistinguishable but show distinct reactions with selected plant hosts (Makkouk et aI., 1988;J.…”

mentioning

confidence: 99%