BRMS1 Regulates Apoptosis in Non-small Cell Lung Cancer Cells

You, Jun; He, Xuejun; Ding, Haibing; Zhang, Tingrong

doi:10.1007/s12013-014-0226-8

Cited by 14 publications

(10 citation statements)

References 19 publications

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“…NSCLC is a widespread metastases and poor prognosis tumor . Recent studies have shown that various types of lncRNAs, such as TBILA, NNT‐AS1, BLACAT1, exhibited biological functions and were involved in the progression of NSCLC.…”

Section: Discussionmentioning

confidence: 99%

Long noncoding RNA PART1 promotes progression of non‐small cell lung cancer cells via JAK‐STAT signaling pathway

Zhu

Wang

et al. 2019

Cancer Medicine

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Non‐small cell lung cancer (NSCLC), the major type of lung cancer, becomes the greatest threat to the life of people. Growing evidence shows prostate androgen‐regulated transcript 1 (PART1) is considered as effective markers for prostate cancer, and has been shown to be associated with poor prognosis of NSCLC. However, the tumorigenic mechanism of PART1 in NSCLC remains to be investigated. In this study, we found that the expression of PART1 was robustly induced in NSCLC tissues and cell lines. Functional studies established that overexpression of PART1 could promote NSCLC cell proliferation, migration, and invasion, while interference of PART1 inhibited NSCLC progression. Our results also identified miR‐635 as a novel target of PART1, whose expression was inhibited by PART1 in NSCLC cell lines. Moreover, gain‐ and loss‐of‐function studies revealed that PART1 could sponge miR‐635 and increase the expression of Janus kinase (JAK) and signal transducer and activator of transcription proteins (STATs). Finally, we deciphered the molecular mechanism by which PART1 contributed to promotion of NSCLC cell progression via phosphorylation and activation of JAK‐STAT signaling pathway. The animal experiment further confirmed that interference of NSCLC could suppress in vivo tumorigenic ability of NSCLC with favorable pharmacological activity via inactivation of JAK‐STAT signaling pathway. In conclusion, our findings clarified the biologic significance of PART1/miR‐635/JAK‐STAT axis in NSCLC progression and provided novel evidence that PART1 may be a new potential therapeutic target for the treatment of NSCLC.

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Section: Discussionmentioning

confidence: 99%

Long noncoding RNA PART1 promotes progression of non‐small cell lung cancer cells via JAK‐STAT signaling pathway

Zhu

Wang

et al. 2019

Cancer Medicine

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“…For instance, Mei et al (35) demonstrated that BRMS1 was able to inhibit the invasion of glioma cells by suppressing urokinase-type plasminogen activator, nuclear factor-κB and the expression and enzymatic activity of matrix metalloproteinase-2 (MMP-2). You et al (36) reported that BRMS1 is able to regulate apoptosis in NSCLC cells by modulating the activation of signal transducer and activator of transcription 3. Apart from the aforementioned signaling pathways, decreased expression of BRMS1 has also been demonstrated to induce the upregulation of human epidermal growth factor receptor 2 (HER2) in breast cancer (37).…”

Section: Discussionmentioning

confidence: 99%

MicroRNA‑125a‑5p inhibits invasion and metastasis of gastric cancer cells by targeting BRMS1 expression

Cao

Tan

et al. 2018

Oncol Lett

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Abstract. Accumulating studies have demonstrated microRNAs (miRNAs/miRs) have an important role in multiple processes of human malignant tumor development and progression. Decreased expression of miR-125a-5p has been observed in several types of cancer, including gastric cancer (GC). However, the mechanism and exact function of miR-125a-5p in GC have not been largely elucidated. In the present study, reverse transcription-quantitative polymerase chain reaction indicated that the expression of miR-125a-5p was downregulated in GC tissues and cell lines compared with matched normal tissues (P<0.01) and normal gastric mucosa cell lines (P<0.01), respectively. Moreover, clinical pathological characteristics and Kaplan-Meier analysis indicated that a low expression of miR-125a-5p was not only associated with lymph metastasis, peritoneal dissemination and advanced tumor-node metastasis stage but also affected the prognosis of GC patients. Compared with miR-control-transfected GC cells, markedly decreased migration and invasion was observed in GC cells that overexpress miR-125a-5p. By contrast, increased metastasis and invasion were observed in miR-125a-5p-knocked down cells compared with the control. Furthermore, luciferase reporter assays indicated that breast cancer metastasis suppressor 1 (BRMS1) was a direct target of miR-125a-5p. Notably, a positive correlation between the levels of BRMS1 and miR-125a-5p in GC tissues was observed, and BRMS1 expression was indicated to be regulated by miR-125a-5p in GC cells. In conclusion, miR-125a-5p may act as a tumor suppressor by targeting the metastasis-inhibitory gene, BRMS1. The data suggesting that BRMS1 is a potential target gene of miR-125a-5p, may provide novel insight into miRNA regulation of human gene expression, and a useful target for gene therapy of GC.

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“…Owing to the high rates of recurrence and metastasis, NSCLC is associated with a strong tendency to metastasize to specific organs ( 32 ). Metastasis, which is a multi-step process involving the proliferation of cancer cells in the primary tumor, local invasion, invasion of lymphatic vessels and blood vessels, local engraftment and the formation of a new tumor (of the same tumor type) at a distal site ( 36 , 37 ), has been estimated to be associated with >70% of all cancer-associated mortalities ( 37 ). Therefore, the identification of molecular factors that predict tumor metastasis is clinically useful, and the identities of these factors may suggest effective therapeutic strategies against NSCLC.…”

Section: Discussionmentioning

confidence: 99%

Role of piwi‑interacting RNA‑651 in the carcinogenesis of non‑small cell lung cancer

et al. 2017

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Piwi-interacting RNAs (piRNAs/piRs) are small non-coding RNAs that can serve important roles in genome stability by silencing transposable genetic elements. piR651, one of these novel piRNAs, regulates a number of biological functions, as well as carcinogenesis. Previous studies have reported that piR651 is overexpressed in human gastric cancer tissues and in several cancer cell lines, including non-small cell lung cancer (NSCLC) cell lines. However, the role of piRNAs in carcinogenesis has not been clearly defined. In the present study, a small interfering RNA inhibitor of piR651 was transfected into the NSCLC A549 and HCC827 cell lines to evaluate the effect of piR651 on cell growth. The association between piR651 expression and apoptosis was evaluated by flow cytometry and western blot analysis. Wound-healing and Transwell migration and invasion assays were used to determine the effect of piR651 on the migration and invasion of NSCLC cell lines. The results revealed that inhibition of piR651 inhibited cell proliferation and significantly increased the apoptotic rate compared with the negative control (NC), as well as altering the expression of apoptosis-associated proteins. There were fewer migrating and invading cells in the piR651-inhibited group than in the NC group in the Transwell assays. Furthermore, in the wound-healing assay, the wound remained wider in the piR651 inhibitor group, suggesting decreased cell migration compared with that in the NC group. The results of the present study demonstrate that piR651 potentially regulates NSCLC tumorigenic behavior by inhibiting cell proliferation, migration and invasion and by inducing apoptosis. Therefore, piR651 is a potential cancer diagnosis marker.

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BRMS1 Regulates Apoptosis in Non-small Cell Lung Cancer Cells

Cited by 14 publications

References 19 publications

Long noncoding RNA PART1 promotes progression of non‐small cell lung cancer cells via JAK‐STAT signaling pathway

Long noncoding RNA PART1 promotes progression of non‐small cell lung cancer cells via JAK‐STAT signaling pathway

MicroRNA‑125a‑5p inhibits invasion and metastasis of gastric cancer cells by targeting BRMS1 expression

Role of piwi‑interacting RNA‑651 in the carcinogenesis of non‑small cell lung cancer

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