BrLAS, a GRAS Transcription Factor From Brassica rapa, Is Involved in Drought Stress Tolerance in Transgenic Arabidopsis

Li, Pan; Zhang, Bin; Su, Tongbing; Li, Pei-Rong; Xin, Xiaoyun; Wang, Weihong; Zhao, Xiuyun; Yu, Yangjun; Zhang, Deshuang

doi:10.3389/fpls.2018.01792

Cited by 45 publications

(28 citation statements)

References 63 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Moreover, GRAS (located in Chr5:M-QTL4 and down-regulated in the roots) proteins belong to a plant-specific transcription factor family involved in many plant processes including plant growth and development as well as abiotic stress responses (Table S6 , Fig. 6 ) [ 49 , 50 ]. It has also been reported that MOC1 encodes a nuclear transcription factor from GRAS family.…”

Section: Discussionmentioning

confidence: 99%

Salt tolerance involved candidate genes in rice: an integrative meta-analysis approach

et al. 2020

View full text Add to dashboard Cite

Background Salinity, as one of the main abiotic stresses, critically threatens growth and fertility of main food crops including rice in the world. To get insight into the molecular mechanisms by which tolerant genotypes responds to the salinity stress, we propose an integrative meta-analysis approach to find the key genes involved in salinity tolerance. Herein, a genome-wide meta-analysis, using microarray and RNA-seq data was conducted which resulted in the identification of differentially expressed genes (DEGs) under salinity stress at tolerant rice genotypes. DEGs were then confirmed by meta-QTL analysis and literature review. Results A total of 3449 DEGs were detected in 46 meta-QTL positions, among which 1286, 86, 1729 and 348 DEGs were observed in root, shoot, seedling, and leaves tissues, respectively. Moreover, functional annotation of DEGs located in the meta-QTLs suggested some involved biological processes (e.g., ion transport, regulation of transcription, cell wall organization and modification as well as response to stress) and molecular function terms (e.g., transporter activity, transcription factor activity and oxidoreductase activity). Remarkably, 23 potential candidate genes were detected in Saltol and hotspot-regions overlying original QTLs for both yield components and ion homeostasis traits; among which, there were many unreported salinity-responsive genes. Some promising candidate genes were detected such as pectinesterase, peroxidase, transcription regulator, high-affinity potassium transporter, cell wall organization, protein serine/threonine phosphatase, and CBS domain cotaining protein. Conclusions The obtained results indicated that, the salt tolerant genotypes use qualified mechanisms particularly in sensing and signalling of the salt stress, regulation of transcription, ionic homeostasis, and Reactive Oxygen Species (ROS) scavenging in response to the salt stress.

show abstract

Section: Discussionmentioning

confidence: 99%

Salt tolerance involved candidate genes in rice: an integrative meta-analysis approach

et al. 2020

View full text Add to dashboard Cite

show abstract

“…By contrast, the fickle N-terminus of GRAS proteins could be folded and modified into specific molecular binding structures. Based on these, the GRAS proteins broadly participated in many critical processes such as signal transductions, root radial elongations, axillary shoot meristem formations and stress responses in plants [ 10 – 13 ]. Previously, the GRAS gene family in Arabidopsis thaliana was separated into eight subfamilies, including DELLA, SCL3, LAS, SCR, HAM, SHR, LISCL and PAT1 [ 14 ].…”

Section: Introductionmentioning

confidence: 99%

Genome-wide identification and characterization of GRAS genes in soybean (Glycine max)

et al. 2020

View full text Add to dashboard Cite

Background: GRAS proteins are crucial transcription factors, which are plant-specific and participate in various plant biological processes. Thanks to the rapid progress of the whole genome sequencing technologies, the GRAS gene families in different plants have been broadly explored and studied. However, comprehensive research on the soybean (Glycine max) GRAS gene family is relatively lagging. Results: In this study, 117 Glycine max GRAS genes (GmGRAS) were identified. Further phylogenetic analyses showed that the GmGRAS genes could be categorized into nine gene subfamilies: DELLA, HAM, LAS, LISCL, PAT1, SCL3, SCL4/7, SCR and SHR. Gene structure analyses turned out that the GmGRAS genes lacked introns and were relatively conserved. Conserved domains and motif patterns of the GmGRAS members in the same subfamily or clade exhibited similarities. Notably, the expansion of the GmGRAS gene family was driven both by gene tandem and segmental duplication events. Whereas, segmental duplications took the major role in generating new GmGRAS genes. Moreover, the synteny and evolutionary constraints analyses of the GRAS proteins among soybean and distinct species (two monocots and four dicots) provided more detailed evidence for GmGRAS gene evolution. Cis-element analyses indicated that the GmGRAS genes may be responsive to diverse environmental stresses and regulate distinct biological processes. Besides, the expression patterns of the GmGRAS genes were varied in various tissues, during saline and dehydration stresses and during seed germination processes. Conclusions: We conducted a systematic investigation of the GRAS genes in soybean, which may be valuable in paving the way for future GmGRAS gene studies and soybean breeding.

show abstract

“…BrLAS is primarily expressed in roots and axillary meristems. Overexpression of the Brassica rapa gene BrLAS in Arabidopsis plants resulted in delayed bolting, flowering time, senescence, decreased fertility and enhanced drought resistance ( Li et al, 2018b ).…”

Section: Introductionmentioning

confidence: 99%

Characterization of the GRAS gene family reveals their contribution to the high adaptability of wheat

Liu¹,

Wang²

2021

PeerJ

View full text Add to dashboard Cite

GRAS transcription factors play important roles in many processes of plant development as well as abiotic and biotic stress responses. However, little is known about this gene family in bread wheat (Triticum aestivum), one of the most important crops worldwide. The completion of a quality draft genome allows genome-wide detection and evolutionary analysis of the GRAS gene family in wheat. In this study, 188 TaGRAS genes were detected and divided into 12 subfamilies based on phylogenetic analyses: DELLA, DLT, HAM, LISCL, SCL3, SCL4/7, SCR, SHR, PAT1, Os19, Os4 and LAS. Tandem and segmental duplications are the main contributors to the expansion of TaGRAS, which may contribute to the adaptation of wheat to various environmental conditions. A high rate of homoeolog retention during hexaploidization was detected, suggesting the nonredundancy and biological importance of TaGRAS homoeologs. Systematic analyses of TaGRAS indicated the conserved expression pattern and function of the same subfamily during evolution. In addition, we detected five genes belonging to the LISCL subfamily induced by both biotic and abiotic stresses and they may be potential targets for further research through gene editing. Using degradome and ChIP-seq data, we identified the targets of miR171 and histone modifications and further analyzed the contribution of epigenetic modification to the subfunctionalization of TaGRAS. This study laid a foundation for further functional elucidation of TaGRAS genes.

show abstract

BrLAS, a GRAS Transcription Factor From Brassica rapa, Is Involved in Drought Stress Tolerance in Transgenic Arabidopsis

Cited by 45 publications

References 63 publications

Salt tolerance involved candidate genes in rice: an integrative meta-analysis approach

Salt tolerance involved candidate genes in rice: an integrative meta-analysis approach

Genome-wide identification and characterization of GRAS genes in soybean (Glycine max)

Characterization of the GRAS gene family reveals their contribution to the high adaptability of wheat

Contact Info

Product

Resources

About