Bringing the multicellular fern meristem into focus

Ambrose, Barbara A.; Vasco, Alejandra

doi:10.1111/nph.13825

Cited by 34 publications

(54 citation statements)

References 35 publications

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“…Furthermore, C3HDZ2 expression throughout the apical dome of leptosporangiate ferns (Fig. 2k,l,o,p) supports the idea of the fern meristem as multicellular with zonation (McAlpin & White, 1974;Stevenson, 1976;Ambrose & Vasco, 2016). Additionally, similar to the findings of Prigge & Clark (2006), we did not detect the expression in the lycophyte S. moellendorffii of any one of the three S. moellendorffii C3HDZs in either vegetative or cone meristems (Figs 3a,d,e, S1g-i).…”

Section: The Ancestral Role Of Vascular Plant Class III Hd-zip Genes supporting

confidence: 85%

Challenging the paradigms of leaf evolution: Class III HD‐Zips in ferns and lycophytes

et al. 2016

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Despite the extraordinary significance leaves have for life on Earth, their origin and development remain vigorously debated. More than a century of paleobotanical, morphological, and phylogenetic research has still not resolved fundamental questions about leaves. Developmental genetic data are sparse in ferns, and comparative studies of lycophytes and seed plants have reached opposing conclusions on the conservation of a leaf developmental program. We performed phylogenetic and expression analyses of a leaf developmental regulator (Class III HD-Zip genes; C3HDZs) spanning lycophytes and ferns. We show that a duplication and neofunctionalization of C3HDZs probably occurred in the ancestor of euphyllophytes, and that there is a common leaf developmental mechanism conserved between ferns and seed plants. We show C3HDZ expression in lycophyte and fern sporangia and show that C3HDZs have conserved expression patterns during initiation of lateral primordia (leaves or sporangia). This expression is maintained throughout sporangium development in lycophytes and ferns and indicates an ancestral role of C3HDZs in sporangium development. We hypothesize that there is a deep homology of all leaves and that a sporangium-specific developmental program was coopted independently for the development of lycophyte and euphyllophyte leaves. This provides molecular genetic support for a paradigm shift in theories of lycophyte leaf evolution.

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Section: The Ancestral Role Of Vascular Plant Class III Hd-zip Genes supporting

confidence: 85%

Challenging the paradigms of leaf evolution: Class III HD‐Zips in ferns and lycophytes

et al. 2016

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“…While gametophyte meristems comprise a single apical stem cell, vascular plant meristems have one to many stem cells capping a more rapidly proliferative zone (figure 3 c , f ) [11,106–108]. The intercalary proliferative zones of bryophyte sporophytes may be homologous to the proliferative zones of vascular plant meristems, but the mechanisms by which apical stem cell activity originated in sporophytes are unknown.…”

Section: Innovations In Vascular Plant Diversificationmentioning

confidence: 99%

Development and genetics in the evolution of land plant body plans

Harrison¹

2017

Phil. Trans. R. Soc. B

146

166

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The colonization of land by plants shaped the terrestrial biosphere, the geosphere and global climates. The nature of morphological and molecular innovation driving land plant evolution has been an enigma for over 200 years. Recent phylogenetic and palaeobotanical advances jointly demonstrate that land plants evolved from freshwater algae and pinpoint key morphological innovations in plant evolution. In the haploid gametophyte phase of the plant life cycle, these include the innovation of mulitcellular forms with apical growth and multiple growth axes. In the diploid phase of the life cycle, multicellular axial sporophytes were an early innovation priming subsequent diversification of indeterminate branched forms with leaves and roots. Reverse and forward genetic approaches in newly emerging model systems are starting to identify the genetic basis of such innovations. The data place plant evo-devo research at the cusp of discovering the developmental and genetic changes driving the radiation of land plant body plans.This article is part of the themed issue ‘Evo-devo in the genomics era, and the origins of morphological diversity’.

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“…While some studies have begun to unravel the developmental role of certain genes in Ceratopteris and other ferns, none of them focused on root cell layers specification (Ambrose and Vasco, 2016;Bui et al, 2017;Cruz et al, 2020;Hasebe et al, 1998;Plackett et al, 2018;Sano et al, 2005;Vasco et al, 2016;Youngstrom et al, 2019;Yu et al, 2020;Zhang et al, 2017).…”

Section: Ceratopteris Root Cell Layers Exhibit the Conservation Of Spmentioning

confidence: 99%

Development and cell cycle dynamics of the root apical meristem in the fernCeratopteris richardii

Aragón-Raygoza

Vasco

Blilou

et al. 2020

Preprint

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Ferns are a representative clade in plant evolution although underestimated in the genomic era. Ceratopteris richardii is an emergent model for developmental processes in ferns, yet a complete scheme of the different growth stages is necessary. Here, we present a developmental analysis, at the tissue and cellular levels, of the first shoot-borne root of Ceratopteris. We followed early stages and emergence of the root meristem in sporelings. While assessing root growth, the first shoot-borne root ceases its elongation between the emergence of the fifth and sixth roots, suggesting Ceratopteris roots follow a determinate developmental program. We report cell division frequencies in the stem cell niche after detecting labeled nuclei in the root apical cell (RAC) and derivatives after 8 hours of exposure. These results demonstrate the RAC has a continuous mitotic activity during root development. Detection of cell cycle activity in the RAC at early times suggests this cell acts as a non-quiescent organizing center. Overall, our results provide a framework to study root function and development in ferns and to better understand the evolutionary history of this organ.Summary StatementIn the Ceratopteris root, the apical cell and its derivatives have a high division frequency, suggesting the apical cell acts as a non-quiescent organizing center in the stem cell niche.

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Bringing the multicellular fern meristem into focus

Cited by 34 publications

References 35 publications

Challenging the paradigms of leaf evolution: Class III HD‐Zips in ferns and lycophytes

Challenging the paradigms of leaf evolution: Class III HD‐Zips in ferns and lycophytes

Development and genetics in the evolution of land plant body plans

Development and cell cycle dynamics of the root apical meristem in the fernCeratopteris richardii

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