The dependence of Brillouin gain spectrum (BGS) characteristics, including the Brillouin frequency shift (BFS) and the BGS bandwidth, on germanium concentration in large-mode-area Ge-doped passive fibers is theoretically and experimentally investigated. Simulation results show that BFS is inversely proportional to GeO2 concentration, and BGS bandwidth increases with the GeO2 concentration initially, and then followed by a decrease. BGS of four fibers with different GeO2 concentration with core diameters of 10 μm and 20 μm are compared experimentally. Experimental results demonstrate that with the same core diameter, the variation of BFS and BGS bandwidth with GeO2 concentration agrees with the simulation results. Additionally, the BGS characteristics of three large-mode-area passive fibers with diameters of 10 μm, 25 μm, and 30 μm are measured, which confirms that increasing the fiber diameters will cause the BGS bandwidth to broaden. We believe that these results can provide valuable references for the modulation of high-power narrow-linewidth fiber lasers and Brillouin fiber amplifiers.