Brillouin Optical Microscopy for Corneal Biomechanics

Scarcelli, Giuliano; Pineda, Roberto; Yun, Seok Hyun

doi:10.1167/iovs.11-8281

Cited by 284 publications

(235 citation statements)

References 47 publications

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“…The stiffness near the bottom surface of the cornea (region IV) turns out to be the lowest, corresponding to the innermost region of the cornea. These results agree well with the observations from the studies using Brillouin optical microscopy on the intact cornea [20] and using mechanical tensile test with the stroma of the cornea [62], which demonstrates the feasibility of the proposed OCE method for depthresolved assessment of corneal biomechanics. Based on the mapping of the Lamb wave phase velocity, Fig.…”

Section: Resultssupporting

confidence: 89%

“…Similar observations are also from the experiments with Brillouin confocal microscopy [20]. More studies with even smaller axial imaging scale combined with theoretical modeling are needed to further investigate this phenomenon.…”

Section: Discussionsupporting

confidence: 77%

“…Scarcelli et al combined the confocal configuration with Brillouin spectroscopy to probe the Brillouin shift caused by the interaction between photons and acoustic phonons in ocular tissues [17,19]. Quantitative imaging of the Brillouin elastic modulus that is related to the sample Young's modulus has been achieved with micro-scale spatial resolution in cornea [20] with the applications demonstrated in the monitoring of collagen cross-linking [21] and keratoconus [22]. This high-resolution mechanical imaging technique has also been applied in the crystalline lens for the spatially-resolved lens stiffness [23,24].…”

Section: Introductionmentioning

confidence: 99%

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Noncontact depth-resolved micro-scale optical coherence elastography of the cornea

Wang

Larin

2014

Biomed. Opt. Express

155

129

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High-resolution elastographic assessment of the cornea can greatly assist clinical diagnosis and treatment of various ocular diseases. Here, we report on the first noncontact depth-resolved micro-scale optical coherence elastography of the cornea achieved using shear wave imaging optical coherence tomography (SWI-OCT) combined with the spectral analysis of the corneal Lamb wave propagation. This imaging method relies on a focused air-puff device to load the cornea with highly-localized lowpressure short-duration air stream and applies phase-resolved OCT detection to capture the low-amplitude deformation with nano-scale sensitivity. The SWI-OCT system is used here to image the corneal Lamb wave propagation with the frame rate the same as the OCT A-line acquisition speed. Based on the spectral analysis of the corneal temporal deformation profiles, the phase velocity of the Lamb wave is obtained at different depths for the major frequency components, which shows the depthwise distribution of the corneal stiffness related to its structural features. Our pilot experiments on ex vivo rabbit eyes demonstrate the feasibility of this method in depth-resolved micro-scale elastography of the cornea. The assessment of the Lamb wave dispersion is also presented, suggesting the potential for the quantitative measurement of corneal viscoelasticity. 3026-3031 (2007). 3. L. T. Nordan, "Keratoconus: diagnosis and treatment," Int. Ophthalmol. Clin. 37(1), 51-63 (1997). 4. C. Edmund, "Corneal elasticity and ocular rigidity in normal and keratoconic eyes," Acta Ophthalmol. (Copenh.) 66(2), 134-140 (1988 Biol. 93(1-3), 176-191 (2007). 17. G. Scarcelli and S. H. Yun, "Confocal Brillouin microscopy for three-dimensional mechanical imaging," Nat. ©2014 Optical Society of AmericaPhotonics 2(1), 39-43 (2008). 18. J. Schmitt, "OCT elastography: imaging microscopic deformation and strain of tissue," Opt. Express 3(6), 199-211 (1998). 19. G. Scarcelli and S. H. Yun, "In vivo Brillouin optical microscopy of the human eye," Opt. Express 20(8), 9197-9202 (2012). 20. G. Scarcelli, R. Pineda, and S. H. Yun, "Brillouin optical microscopy for corneal biomechanics," Invest.Ophthalmol. Vis. Sci. 53(1), 185-190 (2012). 21. G. Scarcelli, S. Kling, E. Quijano, R. Pineda, S. Marcos, and S. H. Yun, "Brillouin microscopy of collagen crosslinking: noncontact depth-dependent analysis of corneal elastic modulus," Invest. Ophthalmol. Vis. Sci.

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Section: Resultssupporting

confidence: 89%

Section: Discussionsupporting

confidence: 77%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Noncontact depth-resolved micro-scale optical coherence elastography of the cornea

Wang

Larin

2014

Biomed. Opt. Express

155

129

View full text Add to dashboard Cite

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“…In recent years, greater attention has been paid to the topic of corneal biomechanics. [1][2][3][4] In vitro experimental studies such as stress-strain tensile tests on corneal strips and inflation tests on the whole cornea have shown the viscoelastic, hyperelastic, and anisotropic features of both animal and human corneas. [5][6][7][8] Electromagnetic radiation scanning approaches are able to demonstrate how the collagen fibrils are spatially arranged within the stroma tissue, which have a great impact on the corneal biomechanical performance.…”

Section: Discussionmentioning

confidence: 99%

Air Puff Induced Corneal Vibrations: Theoretical Simulations and Clinical Observations

Han¹,

Chen²,

Zhou³

et al. 2014

J Refract Surg

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PURPOSE: To investigate air puff induced corneal vibrations and their relationship to the intraocular pressure (IOP), viscoelasticity, mass, and elasticity of the cornea based on theoretical simulations and preliminary clinical observations. METHODS:To simulate the corneal movement during air puff deformation, a kinematic viscoelastic corneal model was developed involving the factors of corneal mass, damping coefficient, elasticity, and IOP. Different parameter values were taken to investigate how factors would affect the corneal movements. Two clinical ocular instruments, CorVis ST (Oculus Optikgeräte GmbH, Wetzlar, Germany) and the Ocular Response Analyzer (ORA; Reichert, Inc., Buffalo, NY), were employed to observe the corneal dynamical behaviors. RESULTS:Numerical results showed that during the air puff deformation, there would be vibrations along with the corneal deformation, and the damping viscoelastic response of the cornea had the potential to reduce the vibration amplitude. With consistent IOP, the overall vibration amplitude and inward motion depths were smaller with a stiffer cornea.CONCLUSIONS: A kinematic viscoelastic model of the cornea is presented to illustrate how the vibrations are associated with factors such as corneal mass, viscoelasticity, and IOP. Also, the predicted corneal vibrations during air puff deformation were confirmed by clinical observation.[J Refract Surg. 2014;30(3):208-213.]

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“…Brillouin spectroscopy is a powerful technique for material characterization [1,2], providing unique information about the mechanical properties of a substance [1], and has found wide usage in remote sensing [3], material science [4,5] and biomedical applications [6,7]. Brillouin scattering originates from the inelastic interaction between the incident electromagnetic wave and the acoustic phonons within the material.…”

Section: Introductionmentioning

confidence: 99%

Background clean-up in Brillouin microspectroscopy of scattering medium

2014

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Abstract:Brillouin spectroscopy is an emerging tool for microscopic optical imaging as it allows for non-contact, non-invasive, direct assessment of the elastic properties of materials. However, strong elastic scattering and stray light from various sources often contaminate the Brillouin spectrum. A molecular absorption cell was introduced into the virtually imaged phased array (VIPA) based Brillouin spectroscopy setup to absorb the Rayleigh component, which resulted in a substantial improvement of the Brillouin spectrum quality.

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Brillouin Optical Microscopy for Corneal Biomechanics

Abstract: Brillouin imaging can assess the biomechanical properties of cornea in situ with high spatial resolution. This novel technique has the potential for use in clinical diagnostics and treatment monitoring.

Cited by 284 publications

References 47 publications

Noncontact depth-resolved micro-scale optical coherence elastography of the cornea

Noncontact depth-resolved micro-scale optical coherence elastography of the cornea

Air Puff Induced Corneal Vibrations: Theoretical Simulations and Clinical Observations

Background clean-up in Brillouin microspectroscopy of scattering medium

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