Brilliant Green Lactose Bile and the Standard Methods Completed Test in Isolation of Coliform Organisms—A Comparative Study

Kelly, C. B.

doi:10.2105/ajph.30.9.1034

Cited by 5 publications

(2 citation statements)

References 3 publications

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“…The failure of coliforms to grow in BGLB when subcultured from lactose-containing media has been well documented (7,26,29,30) and was extremely prevalent when chlorinated potable water samples were examined (30). Even though the suitability of BGLB as a confirmatory medium has been demonstrated (19,21,28), BGLB may not be the best medium to use in the confirmatory test when chlorinated drinking water or untreated surface water supplies are examined.…”

Section: Coliform Maskirng In Confirmatory Mpn Testsmentioning

confidence: 99%

Failure of the most-probable-number technique to detect coliforms in drinking water and raw water supplies

Evans¹,

Waarvick²,

Seidler³

et al. 1981

Appl Environ Microbiol

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A procedure was developed to detect false-negative reactions (interference) in the standard most-probable-number (S-MPN) technique for coliform enumeration of untreated surface water and potable water supplies. This modified MPN (M-MPN) procedure allowed a quantitative assessment of the interference with coliform detection in untreated surface water and potable water supplies. Coliform interference was found to occur in the presumptive, confirmed, and completed tests of the S-MPN technique. When coliforms were present, interference with their detection occurred in over 80% of the samples. The inferior nature of the S-MPN was revealed by the 100% increase in the incidence of completed coliformpositive drinking water samples obtained with the M-MPN technique. The M-MPN procedure was also superior to the standard membrane filter technique. Eight different species of coliforms were recovered from false-negative tests, including Citrobacter, Enterobacter, Klebsiella, and Escherichia coli (in decreasing order of occurrence). The use of standard MPN techniques for monitoring potable water supplies may lead to a false security that the drinking water supply is potable, i.e., free from indicator bacteria.

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Section: Coliform Maskirng In Confirmatory Mpn Testsmentioning

confidence: 99%

Failure of the most-probable-number technique to detect coliforms in drinking water and raw water supplies

Evans¹,

Waarvick²,

Seidler³

et al. 1981

Appl Environ Microbiol

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show abstract

“…The application of these reports to water examination was made by Ellefson (1917, 1919) Several other investigations were made comparing con firmation by BGB2# broth to the completed test (Howard et al. 1941;Kelly, 1940;Smith, 19^1;Taylor, 1940;and Wattie, 1943), In each study, recoveries obtained when BGB2# broth were used were equal to or slightly higher than Metabolic injury has been reviewed by Kueck (1974) of fecal coliforms (1,100 to 1,300/100 ml).…”

mentioning

confidence: 99%