Brilliant cresyl blue staining does not present cytotoxic effects on human luteinized follicular cells, according to gene/protein expression, as well as to cytotoxicity tests

Alcoba, Diego Duarte; Schneider, Júlia; Arruda, Letícia; Martiny, Patrícia Borba; Capp, Edison; Corleta, Helena von Eye; Brum, Ilma Simoni

doi:10.1016/j.repbio.2016.12.003

Cited by 7 publications

(4 citation statements)

References 37 publications

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“…Brilliant cresyl blue (BCB) staining is a non-invasive method for screening for oocyte quality that has been used in different animal species (Roca et al ., 1998; Rodriguez-Gonzalez et al ., 2002; Bhojwani et al ., 2007; Manjunatha et al ., 2007; Wu et al ., 2007a; Ishizaki et al ., 2009; Rodrigues et al ., 2009; Catalá et al ., 2011; Wang et al ., 2012; Silva et al ., 2013; Pereira et al ., 2014). Recent studies on human oocytes have also been reported (Alcoba et al ., 2016, 2017). BCB staining depends on measuring glucose-6-phosphate dehydrogenase (G6PDH) activity.…”

Section: Introductionmentioning

confidence: 99%

Developmental competence of Dromedary camel (Camelus dromedarius) oocytes selected using brilliant cresyl blue staining

Fathi

Ashry

Salama

et al. 2017

Zygote

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The objectives of the present studies were to investigate the developmental capacity of dromedary camel oocytes selected by brilliant cresyl blue (BCB) staining and to investigate the expression of select transcripts in germinal vesicle (GV) stage oocytes. These transcripts included BMP15 and GDF9 as important transcripts for folliculogenesis and oocyte development, Zar1 and Mater as maternal transcripts required for embryonic development, Cyclin B1 and CDK1 as cell cycle regulators and Oct4 and STAT3 as transcription factors. Dromedary camel oocytes were retrieved from ovaries collected at a local slaughterhouse. After exposure to BCB staining, cumulus-oocyte complexes (COCs) from BCB+, BCB- and control (selected based on morphological criteria) groups were subjected to in vitro maturation, in vitro fertilization and in vitro culture. For gene expression studies, after BCB staining cumulus cells were stripped off and the completely denuded GV stage oocytes were used for RT-PCR analysis of selected transcripts. BCB+ oocytes showed higher maturation, and fertilization rates compared with BCB- and control groups. Indices of early embryonic development, namely, cleavage at 48 hours post insemination (hpi), and development to morula at day 5 and day 7 blastocyst rates were also significantly higher in the BCB+ group. RT-PCR revealed a higher expression of BMP15, GDF9, Zar1, Mater, Cyclin B1, CDK1, OCT4 and STAT3 in good quality oocytes that stained positively for BCB (BCB+). Collectively, results provide novel information about the use of BCB screening for selecting good quality oocytes to improve in vitro embryo production in the dromedary camel.

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Section: Introductionmentioning

confidence: 99%

Developmental competence of Dromedary camel (Camelus dromedarius) oocytes selected using brilliant cresyl blue staining

Fathi

Ashry

Salama

et al. 2017

Zygote

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“…Brilliant cresyl blue (BCB) has been reported as a successful non-invasive method in selecting more competent oocytes that develop to the blastocyst stage in different species as cow [7] and goats [8]. Recently, BCB screening studies on human oocytes were reported [9]. Depending on the activity of glucose-6-phosphate dehydrogenase (G6PDH) in the oocytes, BCB can differentiate between more competent oocytes that have lower G6PDH activity and showing blue coloration in their cytoplasm (BCB+) and less competent oocytes that have higher G6PDH activity, which have a reduced blue color to a colorless cytoplasm (BCB−) [9].…”

Section: Introductionmentioning

confidence: 99%

“…Recently, BCB screening studies on human oocytes were reported [9]. Depending on the activity of glucose-6-phosphate dehydrogenase (G6PDH) in the oocytes, BCB can differentiate between more competent oocytes that have lower G6PDH activity and showing blue coloration in their cytoplasm (BCB+) and less competent oocytes that have higher G6PDH activity, which have a reduced blue color to a colorless cytoplasm (BCB−) [9]. Media containing growth factors such as insulin-like growth factor 1 (IGF-1) or epidermal growth factor (EGF) have been reported to improve oocyte maturation, fertilization, and blastocyst development in many species, including sheep [10].…”

Section: Introductionmentioning

confidence: 99%

Effect of adding growth factors during in vitro maturation on the developmental potentials of ewe oocytes selected by brilliant cresyl blue staining

Fathi

El-Karmoty

2021

Vet World

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Aim: Several factors had been concerned with the developmental competence of the sheep oocyte. This study aims to investigate the effect of adding growth factors (insulin-like growth factor 1 [IGF-1] and epidermal growth factor [EGF]) in the maturation medium of ewe oocytes selected based on brilliant cresyl blue (BCB) screening on in vitro maturation (IVM), fertilization, and pre-implantation embryo development. Materials and Methods: Cumulus-oocyte complexes (COCs) were obtained from the ovaries of slaughtered ewes by either aspiration or slicing techniques. COCs were in vitro matured in a medium containing IGF-1 and EGF (control group). For BCB screening, oocytes were stained and divided into BCB+ oocytes that matured in the same maturation conditions without adding growth factors (Group 2) or in the presence of growth factors (Group 3), and BCB– oocytes that matured in medium without growth factors (Group 4) or with growth factors (Group 5). Results: The supplementation of the maturation medium with growth factors during IVM of (BCB+) oocytes resulted in a significant increase in nuclear maturation rate (90.9%), fertilization rate (75.6%), and embryo developmental rates (60.0%, 46.7%, and 33.3% for cleavage, morula, and blastocyst, respectively). Conclusion: Culturing BCB+ oocytes in a maturation medium containing both EGF and IGF-1 showed a significant improvement in nuclear maturation, fertilization, and pre-implantation embryo development in vitro.

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“…2). The dye was selected considering its susceptibility to be oxidized by chemical agents, the difference in the spectrum profile between the oxidized/reduced state, and due to its low toxicity [22].…”

Section: Introductionmentioning

confidence: 99%

Simple colorimetric method to determine the in vitro antioxidant activity of different monoterpenes

Noacco

Rodenak-Kladniew

Bravo

et al. 2018

Analytical Biochemistry

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Brilliant cresyl blue staining does not present cytotoxic effects on human luteinized follicular cells, according to gene/protein expression, as well as to cytotoxicity tests

Cited by 7 publications

References 37 publications

Developmental competence of Dromedary camel (Camelus dromedarius) oocytes selected using brilliant cresyl blue staining

Developmental competence of Dromedary camel (Camelus dromedarius) oocytes selected using brilliant cresyl blue staining

Effect of adding growth factors during in vitro maturation on the developmental potentials of ewe oocytes selected by brilliant cresyl blue staining

Simple colorimetric method to determine the in vitro antioxidant activity of different monoterpenes

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