Background and Aim : AIDS is a progressive, incurable and preventable viral disease that damages the immune system and causes opportunistic infections. The discovery of methods of transmission, prevention and treatment of the disease has prevented researchers from considering the psychological consequences of the disease. The discrimination, adherence to complex treatment regimens, fear of disclosing the disease to the sexual partner exacerbate patients' psychological dissatisfaction and changes their sexual behavior pattern. The present study attempts to investigate the extent of sexual satisfaction of HIV-positive women and their HIV-positive spouses using the Acceptance and Commitment Therapy (ACT). Methods : This double-blind study was performed in 2019 with the aim of investigating the effect of treatment of HIV-positive women and their HIV-positive spouses referred to Behavioral Disease Counseling Center in Yazd and Meybod. 32 eligible women were randomly divided into two groups of 16 and participated in 10 weekly 2-hour sessions. Larsson's demographic and sexual satisfaction questionnaire was used for data collection. Data analysis was done by descriptive statistics and parametric interventions in SPSS software version 16 and significance level 0.05. Findings: There was no significant difference between the two groups in terms of demographic variables. Mean scores of sexual satisfaction before (90.87±13.3) and after intervention (96.31±12.94) and before and one month after the intervention (97.81±12.52) were significantly different in the intervention group. Comparing the mean scores of sexual satisfaction immediately after the intervention and one month later in the intervention group was not significant (P = 0.24). Also, sexual satisfaction was significant between the two groups after the intervention and one month later. Conclusion : The results show that ACT are effective in women with HIV exposure to improve sexual satisfaction. According to the results, it is suggested that this approach be used in order to increase the mental flexibility of patients to accept the disease and the suffering resulting from it, and finally to improve marital relationships and increase sexual satisfaction. In addition, the continuation of sexual health counseling for women is recommended. Trial registration: IRCT20181213041956N1, registered November 2018.