Brief Report: Preservation of the Lactic Dehydrogenase Activity of Platelets by Freezing in Dimethylsulfoxide and Plasma

Abstract

It is concluded that a medium composed of 15 per cent Dimethylsulfoxide and 85 per cent of a mixture of equal parts native plasma and saline effectively preserves platelet lactic acid dehydrogenase activity against slow freezing, storage for 24 hours in temperatures of liquid N2 and fast thawing. Platelet lysis results in a complete loss of LDH activity.

The Effects of Long-term Storage on Commonly Measured Serum Analyte Levels

“…For example, with LDH, rupture of a molecular bond releasing the enzyme from a bound inactive form may be possible. 11, 12…”