Brief molecular diagnostic criteria for human cytomegalovirus infection/disease

Gerna, Giuseppe; Baldanti, Fausto

doi:10.1080/14737159.2019.1657010

Cited by 2 publications

(8 citation statements)

References 29 publications

(28 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Regular post-transplant monitoring of viral replication helps to predict CMV disease risk and guide decisions relating to treatment duration and efficacy (8,9,(16)(17)(18). Monitoring was traditionally undertaken with the pp65 antigenemia assay and qualitative polymerase chain reaction (PCR) (7,19), but there has been a shift toward molecular methods such as quantitative nucleic acid testing. In addition, monitoring CMV-specific T cell immunity post-transplantation is an emerging tool for predicting and controlling CMV infection, and for guiding tailored prevention strategies (8,9,16,20).…”

Section: Introductionmentioning

confidence: 99%

Cytomegalovirus Management in Solid Organ Transplant Recipients: A Pre-COVID-19 Survey From the Working Group of the European Society for Organ Transplantation

et al. 2022

Self Cite

View full text Add to dashboard Cite

Infections are leading causes of morbidity/mortality following solid organ transplantation (SOT) and cytomegalovirus (CMV) is among the most frequent pathogens, causing a considerable threat to SOT recipients. A survey was conducted 19 July–31 October 2019 to capture clinical practices about CMV in SOT recipients (e.g., how practices aligned with guidelines, how adequately treatments met patients’ needs, and respondents’ expectations for future developments). Transplant professionals completed a ∼30-minute online questionnaire: 224 responses were included, representing 160 hospitals and 197 SOT programs (41 countries; 167[83%] European programs). Findings revealed a heterogenous approach to CMV diagnosis and management and, sometimes, significant divergence from international guidelines. Valganciclovir prophylaxis (of variable duration) was administered by 201/224 (90%) respondents in D+/R− SOT and by 40% in R+ cases, with pre-emptive strategies generally reserved for R+ cases: DNA thresholds to initiate treatment ranged across 10–10,000 copies/ml. Ganciclovir-resistant CMV strains were still perceived as major challenges, and tailored treatment was one of the most important unmet needs for CMV management. These findings may help to design studies to evaluate safety and efficacy of new strategies to prevent CMV disease in SOT recipients, and target specific educational activities to harmonize CMV management in this challenging population.

show abstract

Section: Introductionmentioning

confidence: 99%

Cytomegalovirus Management in Solid Organ Transplant Recipients: A Pre-COVID-19 Survey From the Working Group of the European Society for Organ Transplantation

et al. 2022

Self Cite

View full text Add to dashboard Cite

show abstract

“…HCMV latent phases are interspersed by periodic reactivations. In addition, reinfection by different HCMV strains may occur 3 …”

Section: Introductionmentioning

confidence: 99%

“…Sensitive tests for the presence of HCMV infection include enzyme‐linked immunosorbent assay (ELISA) to detect viral antigens using viral protein‐specific monoclonal antibodies (mAbs) 13 . However, the correlation of the presence of antigen and infectious virus and virus replication was found to be low, as during antiviral therapy the disappearance of virus was faster than the reduction in the antigen level 3 . Polymerase chain reaction (PCR) assays for detecting HCMV DNA developed for guiding preemptive treatment of HCMV infections in transplant recipients was found to significantly and safely reduced the number of patients who needed treatment 3 .…”

Section: Introductionmentioning

confidence: 99%

“…In addition, reinfection by different HCMV strains may occur. 3 Sensitive tests for the presence of HCMV infection include enzymelinked immunosorbent assay (ELISA) to detect viral antigens using viral protein-specific monoclonal antibodies (mAbs). 13 However, the correlation of the presence of antigen and infectious virus and virus replication was found to be low, as during antiviral therapy the disappearance of virus was faster than the reduction in the antigen level.…”

Section: Introductionmentioning

confidence: 99%

“…HCMV is widespread worldwide with earlier occurrence in infants and young children in developing countries and a much higher incidence in older age groups in developed countries. 3 The seroprevalence of HCMV in the global adult population is between 45% and 100%. 4 The virus can be found in all organs and can be transmitted by direct contact with bodily fluids such as saliva, tears, urine, stool, semen, and breast milk, and therefore can lead to infection in developing infants 5,6 and in transplant recipients and AIDS patients.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Establishment of a sandwich light‐initiated chemiluminescence assay with double antigen for detecting human cytomegalovirus IgG antibody

Liu

Líu

et al. 2021

Journal of Medical Virology

View full text Add to dashboard Cite

Determination of human cytomegalovirus IgG (HCMV IgG) level is of great importance in the diagnosis of HCMV infections. In this study, a novel, double antigen sandwich homogeneous immunoassay‐based light‐initiated chemiluminescent assay (LICA) for measuring HCMV IgG serum levels was developed. This sandwich LICA for HCMV IgG was performed by incubating serum samples with HCMV pp150 protein coated with chemibeads, streptavidin‐coated sensibeads, and biotinylated HCMV pp150 protein. The working conditions of this assay were optimized and the correlation between the results of the LICA and enzyme‐linked immunosorbent assay was evaluated. As a homogeneous immunoassay, this sandwich LICA could accurately and rapidly determine the serum levels of HCMV IgG with a high‐throughput. Thus, this newly developed assay could be a useful analytical tool in the clinical diagnosis of HCMV infections.

show abstract

Brief molecular diagnostic criteria for human cytomegalovirus infection/disease

Cited by 2 publications

References 29 publications

Cytomegalovirus Management in Solid Organ Transplant Recipients: A Pre-COVID-19 Survey From the Working Group of the European Society for Organ Transplantation

Cytomegalovirus Management in Solid Organ Transplant Recipients: A Pre-COVID-19 Survey From the Working Group of the European Society for Organ Transplantation

Establishment of a sandwich light‐initiated chemiluminescence assay with double antigen for detecting human cytomegalovirus IgG antibody

Contact Info

Product

Resources

About