Brief exposure to directionally-specific pulsed electromagnetic fields stimulates extracellular vesicle release and is antagonized by streptomycin: A potential regenerative medicine and food industry paradigm

Magnetic field therapy enhances muscle mitochondrial bioenergetics and attenuates systemic ceramide levels following ACL reconstruction: Southeast Asian randomized-controlled pilot trial

“…The employed magnetic paradigm produces a low level of oxidative stress that, while adaptive in nature in healthy tissues [ 29 , 64 ], may reach damaging levels in metabolically inflamed tissues [ 65 ]. Therefore, the exposure of an operated limb with aggravated and surgically-inflamed lesions may not have been the best therapeutic approach.…”

“…7 ). In support of potential localized PEMF-induced inflammation, we have recently shown that direct exposure of muscle cells to analogous PEMFs resulted in the induction of oxidative stress that, whilst required to elicit a secretome response for subsequent myogenic enhancement, incurred a small degree of local developmental depression [ 64 ]. By contrast, the provision of PEMF-induced secretome to naïve muscle cells resulted in greater developmental enhancement, without eliciting oxidative stress.…”

“…The PEMF-induced secretome of MSCs has also been previously shown to confer protection against induced inflammation [ 66 ]. In this regard, the systemic effects observed in the present study may thus have arisen from the muscle secretome [ 4 , 5 ] released in response to magnetic field exposure [ 66 , 64 ]. That is, the magnetically-stimulated leg musculature likely provided systemic secretome “feeding” with anti-inflammatory agents that may have underlined our observed metabolic improvements.…”

Magnetic mitohormesis: A non-invasive therapy for inflammatory disorders?

“…In mice, muscular Magnetic Mitohormesis was shown to improve running performance after as little as five weeks of weekly exposure (10 min/wk for a total of 50 min of exposure), enhance muscle oxidative capacity (increase type muscle fiber expression governing aerobic/endurance activities), increase muscle and adipose mitochondriogenesis, induce adipose browning, improve insulin sensitivity, augment fatty acid oxidation, and induce shifts in the microbiome indicative of leaner phenotype as previously described with exercise training (Tai et al, 2020). The observed effects are correlated with the magnetically-induced activation of the muscle secretome (Wong et al, 2022), as previously demonstrated in the stem cell niche with anti-inflammatory attributes (Parate et al, 2020). Indeed, Magnetic Mitohormesis recapitulates many of the hallmark metabolic indices typically associated with habitual exercise (Fig.…”

“…Low amplitude and extremely low-frequency pulsed electromagnetic fields (PEMFs) were shown capable of inducing mitochondrial respiration and mitohormetic responses in muscle, both in vitro (Yap et al, 2019) and in vivo (Tai et al, 2020), downstream of PGC-1α activation. The employed Helmholtz coil systems create a three-dimensional volume of field uniformity (Crocetti et al, 2013;Wong et al, 2022) that is essential for achieving optimal biological efficacy (Parate et al, 2017;Yap et al, 2019;Madanagopal et al, 2021). Muscle was found to be most responsive to PEMFs at an amplitude of 1.5 milliTesla (mT) delivered once a week for 10 min (Yap et al, 2019), whereas smaller or greater amplitude PEMFs, shorter or longer duration, or more frequent exposures, did not render additional benefits or were even less effective.…”