Brief embryonic strychnine exposure in zebrafish causes long-term adult behavioral impairment with indications of embryonic synaptic changes

Roy, Nicole M.; Arpie, Brianna; Lugo, Joseph; Linney, Elwood; Levin, Edward D.; Cerutti, Daniel T.

doi:10.1016/j.ntt.2012.08.001

Cited by 13 publications

(8 citation statements)

References 29 publications

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“…Strychine is a direct glycine antagonist, blocking the binding of glycine to its post-synaptic chloride channel receptor (Hirata et al, 2005). Twenty-four hours pf, zebrafish were exposed to 1.5 mM strychnine for 18 or 29 hours, then raised to adulthood during which swimming ability and behavior in a novel environment were measured (Roy et al, 2012). Adult zebrafish exposed during development to strychnine spent significantly less time at the bottom of the tank than controls and swam faster during the trial than control fish (Roy et al, 2012).…”

Section: Environmental Toxins and Toxicantsmentioning

confidence: 99%

“…Twenty-four hours pf, zebrafish were exposed to 1.5 mM strychnine for 18 or 29 hours, then raised to adulthood during which swimming ability and behavior in a novel environment were measured (Roy et al, 2012). Adult zebrafish exposed during development to strychnine spent significantly less time at the bottom of the tank than controls and swam faster during the trial than control fish (Roy et al, 2012). Strychnine also appeared to alter the expression of genes related to glycinergic, GABAergic and glutaminergic neuronal synapses.…”

Section: Environmental Toxins and Toxicantsmentioning

confidence: 99%

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Zebrafish model systems for developmental neurobehavioral toxicology

Bailey

Oliveri

Levin

2013

Birth Defects Research Pt C

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158

116

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Zebrafish offer many advantages that complement classic mammalian models for the study of normal development as well as for the teratogenic effects of exposure to hazardous compounds. The clear chorion and embryo of the zebrafish allow for continuous visualization of the anatomical changes associated with development, which, along with short maturation times and the capability of complex behavior, makes this model particularly useful for measuring changes to the developing nervous system. Moreover, the rich array of developmental, behavioral, and molecular benefits offered by the zebrafish have contributed to an increasing demand for the use of zebrafish in behavioral teratology. Essential for this endeavor has been the development of a battery of tests to evaluate a spectrum of behavior in zebrafish. Measures of sensorimotor plasticity, emotional function, cognition and social interaction have been used to characterize the persisting adverse effects of developmental exposure to a variety of chemicals including therapeutic drugs, drugs of abuse and environmental toxicants. In this review, we present and discuss such tests and data from a range of developmental neurobehavioral toxicology studies using zebrafish as a model. Zebrafish provide a key intermediate model between high throughput in vitro screens and the classic mammalian models as they have the accessibility of in vitro models and the complex functional capabilities of mammalian models.

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Section: Environmental Toxins and Toxicantsmentioning

confidence: 99%

Section: Environmental Toxins and Toxicantsmentioning

confidence: 99%

Zebrafish model systems for developmental neurobehavioral toxicology

Bailey

Oliveri

Levin

2013

Birth Defects Research Pt C

Self Cite

158

116

View full text Add to dashboard Cite

show abstract

“…There are several assays now reported in the literature to measure behavioral learning changes in adult zebrafish such as the rotating escape test, bite test, novel tank test, place preference test and the T‐maze assay (Levin and Cerutti, ; Stewart et al ., ). Furthermore, the zebrafish is now widely used in pharmacology and toxicology studies (Hill et al ., ) and behavioral and learning assays are being applied to test alterations following drug or chemical exposure (reviewed in Blaser and Vira, ; Eddins et al ., ; Parker et al ., ; Roy et al ., ).…”

Section: Introductionmentioning

confidence: 97%

Directional and color preference in adult zebrafish: Implications in behavioral and learning assays in neurotoxicology studies

Bault

Peterson

Freeman

2015

J of Applied Toxicology

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The zebrafish (Danio rerio) is a useful vertebrate model organism for neurological studies. While a number of behavior and learning assays are recently reported in the literature for zebrafish, many of these assays are still being refined. The initial purpose of this study was to apply a published T-maze assay for adult zebrafish that measures how quickly an organism can discriminate between different color stimuli after receiving reinforcement to measure learning in a study investigating the later life impacts of developmental Pb exposure. The original results were inconclusive as the control group showed a directional and color preference. To assess directional preference further, a three-chambered testing apparatus was constructed and rotated in several directions. The directional preference observed in males was alleviated by rotating the arms pointing west and east. In addition, color preference was investigated using all combinations of five different colors (orange, yellow, green, blue and purple). With directional preference alleviated results showed that both male and female zebrafish preferred colors of shorter wavelengths. An additional experiment tested changes in color preference due to developmental exposure to Pb in adult male zebrafish. Results revealed that Pb-exposed males gained and lost certain color preferences compared to control males and the preference for short wavelengths was decreased. Overall, these results show that consideration and pretesting should be completed before applying behavioral and learning assays involving adult zebrafish to avoid innate preferences and confounding changes in neurotoxicology studies and that developmental Pb exposure alters color preferences in adult male zebrafish.

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“…Over time, damaged neurons impair hippocampal function, gradually leading to memory deficits ( Lazarov and Hollands, 2016 ). Strychnine exposure altered neuronal synapses during embryonic development and impaired motor ability in adulthood ( Roy et al, 2012 ). In our current study, neuronal degeneration was detected by FJ-B staining, which proved that Semen Strychni-induced neurotoxicity could affect the development of neurons and potentially cause cognitive impairment.…”

Section: Discussionmentioning

confidence: 99%

Isoliquiritigenin Alleviates Semen Strychni-Induced Neurotoxicity by Restoring the Metabolic Pathway of Neurotransmitters in Rats

et al. 2021

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Acute neurotoxicity of Semen Strychni can result in sudden death in epilepsy. The detoxification method and mechanism of Semen Strychni acute poisoning have not been clarified. This experiment focused on the mechanism of Semen Strychni neurotoxicity and the alleviation effects of isoliquiritigenin. The rats were intraperitoneally injected with Semen Strychni extract (125 mg/kg), followed by oral administration of isoliquiritigenin (50 mg/kg) for 7 days. FJ-B staining was used to evaluate the degree of injury on hippocampus neurons. The concentration of monoamines, amino acids, and choline neurotransmitters, the Dopamine (DA) and 5-hydroxytryptamine (5-HT) metabolic pathway in the hippocampus, cerebellum, striatum, prefrontal cortex, hypothalamus, serum, and plasma were detected by LC-MS/MS. The expression of neurotransmitter metabolic enzymes [catechol-O-methyl transferase (COMT) and monoamine oxidase (MAO)] and neurotransmitter receptors [glutamate N-methyl-D-aspartic acid receptors (NMDARs) and gamma-aminobutyric acid type A receptor (GABRs)] were, respectively determined using ELISA and qRT-PCR. The results indicated that Semen Strychni induced neuronal degeneration in the hippocampal CA1 region. Meanwhile, Semen Strychni inhibited the mRNA expression of NMDAR1, NMDAR2A, NMDAR2B, GABRa1, GABRb2 and reduced the level of MAO, which disrupted the DA and 5-HT metabolic pathway. However, isoliquiritigenin reversed these effects. In summary, isoliquiritigenin showed alleviation effects on Semen Strychni-induced neurotoxicity, which could be attributed to restoring neurotransmitters metabolic pathway, most likely through the activation of NMDA receptors.

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Brief embryonic strychnine exposure in zebrafish causes long-term adult behavioral impairment with indications of embryonic synaptic changes

Cited by 13 publications

References 29 publications

Zebrafish model systems for developmental neurobehavioral toxicology

Zebrafish model systems for developmental neurobehavioral toxicology

Directional and color preference in adult zebrafish: Implications in behavioral and learning assays in neurotoxicology studies

Isoliquiritigenin Alleviates Semen Strychni-Induced Neurotoxicity by Restoring the Metabolic Pathway of Neurotransmitters in Rats

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