Brief Communication: Cage for Continuous Intravascular Infusion of Isotopes and Drugs in Small Animals2

The transport of methotrexate (MTX) into Walker 256 carcinoma (W256) and hepatoma 5123 (H5123) transplanted in rats was investigated after a pulse injection and continuous infusion of the drug. A mathematical model was developed which adequately described the distribution and transport of MTX in both solid tumors. In H5123 the uptake was limited by the amount of drug carried by plasma (flow-limited transport), but in W256 MTX uptake was limited by the rate at which the drug crossed the tissue barriers (tissue-limited transport). Relative uptake by the solid tumors was almost eightfold more efficient with low than with high doses. MTX concentration in tumor interstitial fluid equilibrated with that of plasma in about 50 hr using a micropore chamber with a diffusion coefficient of 0.5 microm/min as sampling device. MTX concentration was higher in resistant than in responsive tumors.

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Indwelling Vascular Cannulas for Remote Blood Sampling, Infusion, and Long-Term Instrumentation of Small Laboratory Animals

Desjardins

1986

Research Surgery and Care of the Research Animal

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Brief Communication: Cage for Continuous Intravascular Infusion of Isotopes and Drugs in Small Animals2

Abstract: A cage for continuous intra-arterial or intravenous infusion is described. Major features are: a) The animal is free to move during infusion, b) loss of the infused solution is avoided, c) cleaning and decontamination are simple, and d) infusion can continue for weeks.

Cited by 3 publications

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Methods for Vascular Access and Collection of Body Fluids from the Laboratory Rat

Methods for Vascular Access and Collection of Body Fluids from the Laboratory Rat

Pharmacokinetics of methotrexate in solid tumors

Indwelling Vascular Cannulas for Remote Blood Sampling, Infusion, and Long-Term Instrumentation of Small Laboratory Animals

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