Bridging the Gap: A Role for Campylobacter jejuni Biofilms

Tram, Greg; Day, Christopher J.; Korolik, Victoria

doi:10.3390/microorganisms8030452

Cited by 26 publications

(13 citation statements)

References 81 publications

(96 reference statements)

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“…Campylobacter jejuni can form biofilms on different abiotic surfaces used in the food industry (e.g., polystyrene, glass, stainless steel), which is crucial for its environmental survival under varying conditions and thus remains a significant food-safety challenge in the food industry (5). A major problem is also the widespread antibiotic resistance of C. jejuni to a wide range of antibiotics, as well as resistance to various biocides used for disinfection in the food industry (6)(7)(8).…”

Section: Introductionmentioning

confidence: 99%

Antibiofilm Potential of Lavandula Preparations against Campylobacter jejuni

Ramić

Bučar

Kunej

et al. 2021

Appl Environ Microbiol

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New approaches for the control of Campylobacter jejuni biofilms in the food industry are being studied intensively. Natural products are promising alternative antimicrobial substances to control biofilm production, with particular emphasis on plant extracts. Dried flowers of Lavandula angustifolia were used to produce LEO, LEF, and LEW. The chemical compositions determined for these Lavandula preparations included seven major compounds that were selected for further testing. These were tested against C. jejuni , for biofilm degradation and removal. Next-generation sequencing was used to study the molecular mechanisms underlying LEO actions against C. jejuni adhesion and motility. Analysis of LEO revealed 1,8-cineol, linalool and linalyl acetate as the main components. For LEF and LEW, the main components were phenolic acid glycosides, with flavonoids rarely present. The minimal inhibitory concentrations of the Lavandula preparations and pure compounds against C. jejuni ranged from 0.2 mg/mL to 1 mg/mL. LEO showed the strongest biofilm degradation. The reduction of C. jejuni adhesion was by ≥1 log 10 CFU/mL, which satisfies European Food Safety Authority recommendations. Lavandula preparations reduced C. jejuni motility by almost 50%, which consequently can impact upon biofilm formation. These data are in line with the transcriptome analysis of C. jejuni , where LEO down-regulated genes important for biofilm formation. LEW also showed good antibacterial and anti-biofilm effects, particularly against adhesion and motility mechanisms. This defines an innovative approach using alternative strategies and novel targets to combat bacterial biofilm formation, and hence the potential to develop new effective agents with biofilm-degrading activities. Importance The Lavandula preparations used in this study are found to be effective against C. jejuni , a common foodborne pathogen. They show anti-biofilm properties at sub-inhibitory concentrations in terms of promoting biofilm degradation and inhibiting cell adhesion and motility, which are involved in the initial steps of biofilm formation. These results are confirmed by transcriptome analysis, which highlights the effect of Lavandula essential oil on C. jejuni biofilm properties. We show that the waste material from the hydrodistillation of Lavandula has particular anti-biofilm effects, suggesting that it may find reuse for industrial purposes. This study highlights the need for efforts directed towards such innovative approaches and alternative strategies against biofilm formation and maintenance by developing new naturally derived agents with anti-biofilm activities.

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Section: Introductionmentioning

confidence: 99%

Antibiofilm Potential of Lavandula Preparations against Campylobacter jejuni

Ramić

Bučar

Kunej

et al. 2021

Appl Environ Microbiol

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“…Despite it being a fastidious organism, C. jejuni can survive outside the avian intestinal tract before it reaches a human host. A range of environmental elements initiates the formation of biofilms, which are then affected by a set of intrinsic factors [ 57 ]. The European Union One Health 2018 Zoonoses Report classifies C. jejuni as an opportunistic pathogen that is believed to be the causative agent of most bacterial gastroenteritis cases, and has been regarded as a common commensal of food animals and poultry, with turkeys and hens in particular [ 42 ].…”

Section: Examples Of the Most Relevant Biofilms In The Food Industmentioning

confidence: 99%

Microbial Biofilms in the Food Industry—A Comprehensive Review

Carrascosa

Raheem

Ramos

et al. 2021

IJERPH

220

109

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Biofilms, present as microorganisms and surviving on surfaces, can increase food cross-contamination, leading to changes in the food industry’s cleaning and disinfection dynamics. Biofilm is an association of microorganisms that is irreversibly linked with a surface, contained in an extracellular polymeric substance matrix, which poses a formidable challenge for food industries. To avoid biofilms from forming, and to eliminate them from reversible attachment and irreversible stages, where attached microorganisms improve surface adhesion, a strong disinfectant is required to eliminate bacterial attachments. This review paper tackles biofilm problems from all perspectives, including biofilm-forming pathogens in the food industry, disinfectant resistance of biofilm, and identification methods. As biofilms are largely responsible for food spoilage and outbreaks, they are also considered responsible for damage to food processing equipment. Hence the need to gain good knowledge about all of the factors favouring their development or growth, such as the attachment surface, food matrix components, environmental conditions, the bacterial cells involved, and electrostatic charging of surfaces. Overall, this review study shows the real threat of biofilms in the food industry due to the resistance of disinfectants and the mechanisms developed for their survival, including the intercellular signalling system, the cyclic nucleotide second messenger, and biofilm-associated proteins.

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“…LuxS/AI-2 system plays important roles in cell-cell interactions in C. jejuni [105,106]. Because biofilm is crucial for C. jejuni survive outside of hosts and facilitates its transmission from chicken reservoirs to humans, LuxS-mutant strains show deficient in biofilm formation and possible reduction of their transmission [107]. C. jejuni 81176 luxS mutant shows significant decreased colonization in chickens [108].…”

Section: Microbial Metabolites and Campylobacteriosismentioning

confidence: 99%

The Role of Immune Response and Microbiota on Campylobacteriosis

Fu¹,

Alenezi²,

Almansour³

et al. 2022

Campylobacter

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Million cases of campylobacteriosis and complications of post-Campylobacter jejuni infection occur every year around the world with huge life losses and economic burdens of billions of dollars. Few therapy options, such as antibiotics, are available to relieve severe cases of the enteritis. The slow progression on new intervention discovery and application is partially resulted from limited mechanistic understanding on campylobacteriosis pathogenesis. As a type of intestinal disorders, campylobacteriosis shares many common features with other intestinal diseases such as inflammatory bowel diseases (IBD) and Clostridium difficile infection. In pace with the advancement of the gastroenterology field, a large body of knowledge is accumulating on the factors influencing campylobacteriosis onset, development, and outcomes, including host immune response, intestinal microbiota, and its metabolites. In this chapter, we review the intestinal immune system, intestinal microbiome, and microbiome modulation of inflammation in the development of campylobacteriosis. The interplay between immunity, microbiota, and its metabolites may play essential roles on campylobacteriosis pathogenesis and the finding on the interaction may lead to new prevention and treatment options. The purpose of this chapter is to provide updated knowledge on the role of host–microbe interaction and the therapeutic potential on campylobacteriosis.

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Bridging the Gap: A Role for Campylobacter jejuni Biofilms

Cited by 26 publications

References 81 publications

Antibiofilm Potential of Lavandula Preparations against Campylobacter jejuni

Antibiofilm Potential of Lavandula Preparations against Campylobacter jejuni

Microbial Biofilms in the Food Industry—A Comprehensive Review

The Role of Immune Response and Microbiota on Campylobacteriosis

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