Bridging Dormancy Release and Apical Dominance in Potato Tuber

Eshel, Dani

doi:10.1007/978-3-319-14451-1_11

Cited by 8 publications

(6 citation statements)

References 68 publications

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“…Campbell et al () observed that transcript profiles during BE‐induced cessation of dormancy are similar to those observed in natural dormancy release, suggesting that both follow a similar biological pattern during this transition. Thus, BE treatment can be used to compress and synchronize release from the dormant period, which is an advantage from an experimental standpoint (Campbell et al, ; Eshel, ).…”

Section: Introductionmentioning

confidence: 99%

Vacuolar processing enzyme activates programmed cell death in the apical meristem inducing loss of apical dominance

Teper‐Bamnolker

Buskila

Belausov

et al. 2017

Plant Cell & Environment

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The potato (Solanum tuberosum L.) tuber is a swollen underground stem that can sprout in an apical dominance (AD) pattern. Bromoethane (BE) induces loss of AD and the accumulation of vegetative vacuolar processing enzyme (S. tuberosum vacuolar processing enzyme [StVPE]) in the tuber apical meristem (TAM). Vacuolar processing enzyme activity, induced by BE, is followed by programmed cell death in the TAM. In this study, we found that the mature StVPE1 (mVPE) protein exhibits specific activity for caspase 1, but not caspase 3 substrates. Optimal activity of mVPE was achieved at acidic pH, consistent with localization of StVPE1 to the vacuole, at the edge of the TAM. Downregulation of StVPE1 by RNA interference resulted in reduced stem branching and retained AD in tubers treated with BE. Overexpression of StVPE1 fused to green fluorescent protein showed enhanced stem branching after BE treatment. Our data suggest that, following stress, induction of StVPE1 in the TAM induces AD loss and stem branching.

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Section: Introductionmentioning

confidence: 99%

Vacuolar processing enzyme activates programmed cell death in the apical meristem inducing loss of apical dominance

Teper‐Bamnolker

Buskila

Belausov

et al. 2017

Plant Cell & Environment

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“…The potato tuber, a swollen stem, stored at 14°C tends to sprout, when dormancy is released from the apical bud, in an AD form (Teper-Bamnolker et al, 2012). Sprouts growing from the tuber nodes (eyes) indicate loss of AD (Eshel, 2015). Seeking environmental factors that induce loss of AD, we heated nonsprouting tubers, after incubating them at 14°C for 4 to 5 weeks, when they are developmentally in shallow dormancy.…”

Section: Heat Treatment Induces Stem Branchingmentioning

confidence: 99%

“…During tuber development, a growing number of axillary buds (termed eyes) are formed in a spiral arrangement on its surface, while the apical bud is located at the tip of the stolon (Goodwin, 1967). During sprouting, the tuber exhibits an AD phenomenon, similar to a normal stem (Eshel and Teper-Bamnolker, 2012;Teper-Bamnolker et al, 2012;Eshel, 2015). During tuber development, the storage parenchyma converts soluble assimilates (i.e.…”

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confidence: 99%

Etiolated Stem Branching Is a Result of Systemic Signaling Associated with Sucrose Level

et al. 2017

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The potato () tuber is a swollen stem. Sprouts growing from the tuber nodes represent loss of apical dominance and branching. Long cold storage induces loss of tuber apical dominance and results in secondary branching. Here, we show that a similar branching pattern can be induced by short heat treatment of the tubers. Detached sprouts were induced to branch by the heat treatment only when attached to a parenchyma cylinder. Grafting experiments showed that the scion branches only when grafted onto heat- or cold-treated tuber parenchyma, suggesting that the branching signal is transmitted systemically from the bud-base parenchyma to the grafted stem. Exogenous supply of sucrose (Suc), glucose, or fructose solution to detached sprouts induced branching in a dose-responsive manner, and an increase in Suc level was observed in tuber parenchyma upon branching induction, suggesting a role for elevated parenchyma sugars in the regulation of branching. However, sugar analysis of the apex and node after grafting showed no distinct differences in sugar levels between branching and nonbranching stems. Vacuolar invertase is a key enzyme in determining the level of Suc and its cleavage products, glucose and fructose, in potato parenchyma. Silencing of the vacuolar invertase-encoding gene led to increased tuber branching in combination with branching-inducing treatments. These results suggest that Suc in the parenchyma induces branching through signaling and not by excess mobilization from the parenchyma to the stem.

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“…Physical and chemical agents are currently used for controlling the length of the dormancy period. One of the most usual practices is to keep the harvested potatoes at a low storage temperature, delaying the germination of the apical bud [ 13 , 14 ]. Phytotoxic chemical sprout suppressors, such as chlorpropham (CIPC), dimethylnaphthalene and maleic hydrazide, are also used to delay the exit of dormancy [ 6 , 15 ].…”

Section: Introductionmentioning

confidence: 99%

Comparative Proteomics of Potato Cultivars with a Variable Dormancy Period

et al. 2022

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The control of the duration of the dormancy phase is a significant challenge in the potato industry and for seed producers. However, the proteome landscape involved in the regulation of the length of the dormancy period over potato cultivars remains largely unexplored. In this study, we performed for the first time a comparative proteome profiling of potato cultivars with differential duration of tuber dormancy. More specifically, the proteome profiling of Agata, Kennebec and Agria commercial potato varieties with short, medium and medium-long dormancy, respectively, was assessed at the endodormancy stage using high-resolution two-dimensional electrophoresis (2-DE) coupled to reversed-phase liquid chromatography–tandem mass spectrometry (LC-TripleTOF MS/MS). A total of 11 proteins/isoforms with statistically significant differential abundance among cultivars were detected on 2-DE gels and confidently identified by LC-TripleTOF MS/MS. Identified proteins have known functions related to tuber development, sprouting and the oxylipins biosynthesis pathway. Fructokinase, a mitochondrial ADP/ATP carrier, catalase isozyme 2 and heat shock 70 kDa were the proteins with the strongest response to dormancy variations. To the best of our knowledge, this study reports the first candidate proteins underlying variable dormancy length in potato cultivars.

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Bridging Dormancy Release and Apical Dominance in Potato Tuber

Cited by 8 publications

References 68 publications

Vacuolar processing enzyme activates programmed cell death in the apical meristem inducing loss of apical dominance

Vacuolar processing enzyme activates programmed cell death in the apical meristem inducing loss of apical dominance

Etiolated Stem Branching Is a Result of Systemic Signaling Associated with Sucrose Level

Comparative Proteomics of Potato Cultivars with a Variable Dormancy Period

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