BRG1 Interacts with Nrf2 To Selectively Mediate <i>HO-1</i> Induction in Response to Oxidative Stress

Zhang, Jianyong; Ohta, Tsutomu; Maruyama, Atsushi; Hosoya, Takamitsu; Nishikawa, Keizo; Maher, Jonathan; Shibahara, Shigeki; Itoh, Ken; Yamamoto, Masayuki

doi:10.1128/mcb.00700-06

Cited by 181 publications

(195 citation statements)

References 62 publications

(67 reference statements)

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“…A role for Z-DNA in chromatin remodeling and transcriptional activation in vivo has been demonstrated in the human CSF1 promoter, where transcriptional activation by the human BAF complex depends on the presence of a Z-forming TG/CA dinucleotide repeat sequence near the promoter (12). Similar results were found with the human heme oxygenase HO-1 gene (14). The presence of this Z-forming DNA sequence was shown to increase nuclease accessibility of the promoter DNA region of CSF1, which is associated with a nucleosome in the basal state (23).…”

Section: The Extent Of Transcriptional Stimulation By Upstream Promoter-supporting

confidence: 61%

“…This result suggests that the d(TG/CA) repeat sequence, which is known to form Z-DNA less readily than d(CG) repeats do, may require additional factors, such as the ATP-hydrolyzing chromatin-remodeling complex, to generate higher levels of negative supercoiling to form Z-DNA. Interestingly, a d(TG/ CA) dinucleotide repeat sequence is located 170 bp upstream of the CATA box of the human heme oxygenase HO-1 gene (14). This separation distance corresponds to the length of one nucleosomal DNA repeat, including linker DNA, which places the CATA box at the DNA-entry site of the second downstream nucleosome from the Z-DNA-forming sequence.…”

Section: Discussionmentioning

confidence: 99%

“…Formation of Z-DNA in vivo requires negative DNA supercoiling (9,10), which can be supplied by a moving RNA polymerase. Z-DNA formation at a promoter region has been shown to correlate with transcriptional activity (5,(11)(12)(13)(14). Although Z-DNA often acts as an enhancer of transcription, its formation in the upstream regulatory region of the rat nucleolin gene decreases the level of expression (15).…”

mentioning

confidence: 99%

“…The role of Z-DNA in chromatin remodeling and enhancement of transcription has been demonstrated in the human CSF1 promoter, where activation requires the cooperation among the transcription factor nuclear factor I, the chromatin-remodeling BAF complex, and an upstream promoterproximal Z-DNA structure (12,23). More recently, induction of the human heme oxygenase HO-1 gene has been shown to require Z-DNA formation near the promoter region (14).…”

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confidence: 99%

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Characterization of Z-DNA as a nucleosome-boundary element in yeastSaccharomyces cerevisiae

Wong

Chen

Kwon

et al. 2007

Proc. Natl. Acad. Sci. U.S.A.

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In this article, the effect of a d(CG) DNA dinucleotide repeat sequence on RNA polymerase II transcription is examined in yeast Saccharomyces cerevisiae. Our previous report shows that a d(CG)n dinucleotide repeat sequence located proximally upstream of the TATA box enhances transcription from a minimal CYC1 promoter in a manner that depends on its surrounding negative supercoiling. Here, we demonstrate that the d(CG)9 repeat sequence stimulates gene activity by forming a Z-DNA secondary structure. Furthermore, the extent of transcriptional enhancement by Z-DNA is promoter-specific and determined by its separation distance relative to the TATA box. The stimulatory effect exerted by promoter proximal Z-DNA is not affected by helical phasing relative to the TATA box, suggesting that Z-DNA effects transcription without interacting with the general transcription machinery by loopingout the intervening DNA. A nucleosome-scanning assay reveals that the d(CG)9 repeat sequence in the Z conformation blocks nucleosome formation, and it is found in the linker DNA with two flanking nucleosomes. This result suggests that Z-DNA formation proximally upstream of a promoter is sufficient to demarcate the boundaries of its neighboring nucleosomes, which produces transcriptionally favorable locations for the TATA box near the nucleosomal DNA-entry site and at dyad positions on the nucleosome. These findings suggest that Z-DNA formation in chromatin is a part of the ''genomic code'' for nucleosome positioning in vivo.chromatin remodeling ͉ promoter ͉ transcription ͉ nucleosome positioning

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Section: The Extent Of Transcriptional Stimulation By Upstream Promoter-supporting

confidence: 61%

Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

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Characterization of Z-DNA as a nucleosome-boundary element in yeastSaccharomyces cerevisiae

Wong

Chen

Kwon

et al. 2007

Proc. Natl. Acad. Sci. U.S.A.

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“…Nuclear extracts were prepared from primary megakaryocytes as described before, 27 and immunoblot analysis was performed using anti-Nrf2, 28 anti-p45 (Santa Cruz Biotechnology; sc-291), and anti-LaminB (Santa Cruz Biotechnology; sc-6217). Whole-cell extracts were prepared from transiently transfected 293T cells, and immunoblot analysis was performed with anti-Flag antibody (Sigma-Aldrich; F3165).…”

Section: Immunoblot Analysismentioning

confidence: 99%

NF-E2 domination over Nrf2 promotes ROS accumulation and megakaryocytic maturation

Motohashi

Kimura

Fujita

et al. 2010

Blood

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In megakaryocytes, the maturation process and oxidative stress response appear to be closely related. It has been suggested that increased oxygen tension and reactive oxygen species (ROS) promote megakaryopoiesis and that the expression of stress-responsive genes responsible for ROS elimination declines during megakaryocytic maturation. NF-E2 p45 is an essential regulator of megakaryopoiesis, whereas Nrf2 is a key activator of stress-responsive genes. Because p45 and Nrf2 have similar DNAbinding specificities, we hypothesized that p45 competes with Nrf2 to repress stress-responsive genes and achieves favorable intracellular conditions to allow ROS to be efficiently used as signaling molecules. We conducted comprehensive gene expression profiling with wildtype and p45-null megakaryocytes and examined the functional relationship between p45 and Nrf2. We found that 2 characteristic gene clusters are defined within p45 target genes: platelet genes and cytoprotective genes. The former are unique targets activated by p45, whereas the latter are common targets of p45 and Nrf2. Further analysis suggested that, as a less efficacious activator, p45 maintains moderate expression of cytoprotective genes through competing with Nrf2 and promotes ROS accumulation. Increased ROS enhanced platelet gene expression. These results suggest that p45 dominates over Nrf2 to enhance megakaryocytic maturation by promoting ROS accumulation. (Blood. 2010;115:677-686)

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Genetic, metabolic and immunological features of cancers with NRF2 addiction

2022

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Nuclear factor erythroid‐derived 2‐like 2 (NRF2) is a master transcription factor that coordinately regulates the expression of many cytoprotective genes and plays a central role in defense mechanisms against oxidative and electrophilic insults. Although increased NRF2 activity is principally beneficial for our health, NRF2 activation in cancer cells is detrimental. Many human cancers exhibit persistent NRF2 activation and such cancer cells rely on NRF2 for most of their malignant characteristics, such as therapeutic resistance and aggressive tumourigenesis, and thus fall into NRF2 addiction. The persistent activation of NRF2 confers great advantages on cancer cells, whereas it is not tolerated by normal cells, suggesting that certain requirements are necessary for a cell to exploit NRF2 and evolve into malignant cancer cells. In this review, recent reports and data on the genetic, metabolic and immunological features of NRF2‐activated cancer cells are summarized, and prerequisites for NRF2 addiction in cancer cells and their therapeutic applications are discussed.

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BRG1 Interacts with Nrf2 To Selectively Mediate HO-1 Induction in Response to Oxidative Stress

Cited by 181 publications

References 62 publications

Characterization of Z-DNA as a nucleosome-boundary element in yeastSaccharomyces cerevisiae

Characterization of Z-DNA as a nucleosome-boundary element in yeastSaccharomyces cerevisiae

NF-E2 domination over Nrf2 promotes ROS accumulation and megakaryocytic maturation

Genetic, metabolic and immunological features of cancers with NRF2 addiction

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