Breznakia blatticola gen. nov. sp. nov. and Breznakia pachnodae sp. nov., two fermenting bacteria isolated from insect guts, and emended description of the family Erysipelotrichaceae

Tegtmeier, Dorothee; Riese, Cornelius; Geissinger, Oliver; Radek, Renate; Brune, Andreas

doi:10.1016/j.syapm.2016.05.003

Cited by 50 publications

(27 citation statements)

References 55 publications

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“…Within Firmicutes, bacteria from the family Veillonellaceae (26%), and Erysipelotrichaceae (9%) were the most abundant; and the same occurred for the genus Desulfovibrio (3%), from the Proteobacteria phylum (data not shown). The microbial composition after the injection of lactate was dominated by fermenting bacteria that were probably induced by the injection of lactate (Fennell et al, 1997;He et al, 2007;Tegtmeier et al, 2016). In contrast, the abundance of the Firmicutes phylum was very limited in well PZ-1 (abundance of 4% at t142) and almost insignificant in well PZ-22, which agrees with the chemical and isotopic results discussed before.…”

Section: Impact Of the In-situ Erd Pilot Test At Wells Within The Dirsupporting

confidence: 87%

Integrative isotopic and molecular approach for the diagnosis and implementation of an efficient in-situ enhanced biological reductive dechlorination of chlorinated ethenes

Blázquez-Pallí

Rosell

Varias³

et al. 2019

Water Research

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Based on the previously observed intrinsic bioremediation potential of a site originally contaminated with perchloroethene (PCE), field-derived lactate-amended microcosms were performed to test different lactate isomers and concentrations, and find clearer isotopic and molecular parameters proving the feasibility of an in-situ enhanced reductive dechlorination (ERD) from PCE-to-ethene (ETH). According to these laboratory results, which confirmed the presence of Dehalococcoides sp. and the vcrA gene, an in-situ ERD pilot test consisting of a single injection of lactate in a monitoring well was performed and monitored for 190 days. The parameters used to follow the performance of the ERD comprised the analysis of i) hydrochemistry, including redox potential (Eh), and the concentrations of redox sensitive species, chlorinated ethenes (CEs), lactate, and acetate; ii) stable isotope composition of carbon of CEs, and sulphur and oxygen of sulphate; and iii) 16S rRNA gene sequencing from groundwater samples. Thus, it was proved that the injection of lactate promoted sulphate-reducing conditions, with the subsequent decrease in Eh, which allowed for the full reductive dechlorination of PCE to ETH in the injection well. The biodegradation of CEs was also confirmed by the enrichment in 13 C and carbon isotopic mass balances. The metagenomic results evidenced the shift in the composition of the microbial population towards the predominance of fermentative bacteria. Given the success of the in-situ pilot test, a full-scale ERD with lactate was then implemented at the site. After one year of treatment, PCE and trichloroethene were mostly depleted, whereas vinyl chloride (VC) and ETH were the predominant metabolites. Most importantly, the shift of the carbon isotopic mass balances towards more positive values confirmed the complete reductive dechlorination, including the VC-to-ETH reaction step. The combination of techniques used here provides complementary lines of evidence for the diagnosis of the intrinsic biodegradation potential of a polluted site, but also to monitor 3 the progress, identify potential difficulties, and evaluate the success of ERD at the field scale.

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Section: Impact Of the In-situ Erd Pilot Test At Wells Within The Dirsupporting

confidence: 87%

Integrative isotopic and molecular approach for the diagnosis and implementation of an efficient in-situ enhanced biological reductive dechlorination of chlorinated ethenes

Blázquez-Pallí

Rosell

Varias³

et al. 2019

Water Research

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“…Of the RUGs that checkM could only resolve to the Bacteria kingdom, we noticed that 31 of them had significant numbers of protein hits to the Solobacterium , Coprobacillus and Kandleria genera, which are all members of the Erysipelotrichales order. Members of the Erysipelotrichales have been isolated from the human 11 , mouse 46 and insect gut 47 and pig manure 48 . We hypothesized that some of the 31 RUGs may represent new clades within Erysipelotrichales .…”

Section: Resultsmentioning

confidence: 99%

“…The coprobacilli are a diverse and somewhat ill-defined group. Indeed, many former members of the Erysipelotrichaceae have been suggested to be members of a new family, Coprobacillaceae 47 , within the Erysipelotrichales Order, and the above RUGs cluster more closely with this clade. We therefore propose the name 'uncultured rumen Coprobacillaceae ' for these RUGs.…”

Section: Resultsmentioning

confidence: 99%

Assembly of 913 microbial genomes from metagenomic sequencing of the cow rumen

et al. 2018

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The cow rumen is adapted for the breakdown of plant material into energy and nutrients, a task largely performed by enzymes encoded by the rumen microbiome. Here we present 913 draft bacterial and archaeal genomes assembled from over 800 Gb of rumen metagenomic sequence data derived from 43 Scottish cattle, using both metagenomic binning and Hi-C-based proximity-guided assembly. Most of these genomes represent previously unsequenced strains and species. The draft genomes contain over 69,000 proteins predicted to be involved in carbohydrate metabolism, over 90% of which do not have a good match in public databases. Inclusion of the 913 genomes presented here improves metagenomic read classification by sevenfold against our own data, and by fivefold against other publicly available rumen datasets. Thus, our dataset substantially improves the coverage of rumen microbial genomes in the public databases and represents a valuable resource for biomass-degrading enzyme discovery and studies of the rumen microbiome.

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“…To select bacterial strains with antifungal activity, gut homogenates and aqueous fecal extracts were diluted to 1 × 10 5 and 1 × 10, respectively. Fifty bacterial colonies were selected based on their color, morphology and size using successive streaking in a PDA medium . The purified strains were stored in 30% glycerol at −80 °C and were revived in a PDA medium .…”

Section: Methodsmentioning

confidence: 99%

The interactions between gut microbiota and entomopathogenic fungi: a potential approach for biological control of Blattella germanica (L.)

Zhang

Sun

Zhang

et al. 2017

Pest Management Science

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Breznakia blatticola gen. nov. sp. nov. and Breznakia pachnodae sp. nov., two fermenting bacteria isolated from insect guts, and emended description of the family Erysipelotrichaceae

Cited by 50 publications

References 55 publications

Integrative isotopic and molecular approach for the diagnosis and implementation of an efficient in-situ enhanced biological reductive dechlorination of chlorinated ethenes

Integrative isotopic and molecular approach for the diagnosis and implementation of an efficient in-situ enhanced biological reductive dechlorination of chlorinated ethenes

Assembly of 913 microbial genomes from metagenomic sequencing of the cow rumen

The interactions between gut microbiota and entomopathogenic fungi: a potential approach for biological control of Blattella germanica (L.)

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