Brewery wastewater treatment using cyanobacterial-bacterial settleable aggregates

Papadopoulos, Konstantinos P.; Economou, Christina Ν.; Dailianis, Stefanos; Charalampous, Nikolina; Stefanidou, Natassa; Moustaka‐Gouni, Maria; Tekerlekopoulou, Athanasia G.; Vayenas, D.V.

doi:10.1016/j.algal.2020.101957

Cited by 36 publications

(25 citation statements)

References 63 publications

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“…The culture also contained a significant number of heterotrophic bacteria and protozoa (relative biomass fraction: 10%). Microbial population percentages are expressed as fractions of the total biomass of the microbial community, including cyanobacteria filaments and large-sized heterotrophic bacteria (1-2 µm) [4,5]. The culture was then acclimatized in the brewery wastewater (Section 2.1), under agitation and the conditions described above, without any significant alterations in microbial population composition.…”

Section: Culture Identification and Inoculum Preparationmentioning

confidence: 99%

“…After the end of each experiment, the treated wastewater was drained into the buffer vessel and biomass was harvested from the growth surface by scraping. After re-suspension in 50 mL of distilled water, it was centrifuged, washed twice to remove nutrient residues and other impurities, and dried overnight at 105 • C. Previous studies utilizing the same Leptolyngbya-based microbial consortium have shown that it accumulates up to 40% of its dry biomass weight in carbohydrates [4,5], and for this reason carbohydrates were chosen as the target biomass compound of the present study. Intracellular sugar content (expressed as glucose equivalents) was estimated photometrically according to Dubois et al [32].…”

Section: Biomass Harvesting and Determination Of Intracellular Compoundsmentioning

confidence: 99%

“…Firstly, 1 mg of dried biomass reduced to powder was mixed with 5 mL of distilled water. Then, 1 mL of this suspension was then mixed with 1 mL of 5% (w/v) phenol solution and 5 mL sulfuric acid solution and measured at 490 nm after 30 min [4,5,32]. To determine protein content, 10 mg of dried harvested biomass were suspended into 5 mL of phosphate buffer solution (pH = 7.3).…”

Section: Biomass Harvesting and Determination Of Intracellular Compoundsmentioning

confidence: 99%

“…Wastewater treatment systems utilizing photosynthetic microorganisms have recently emerged as a promising alternative to conventional biological processes such as activated sludge [3]. However, the treatment of agro-industrial, industrial and domestic wastewaters with algae/cyanobacteria has gathered significant research interest over the last decade and produced promising results in both organic and inorganic pollutant removal [4][5][6][7][8][9]. This method offers several advantages, such as reduced operational cost as photosynthesis replaces mechanical aeration requirements, decreased CO 2 emissions [10], and the high cost of using chemical fertilizers for microalgal growth is avoided since microorganisms use the nutrients contained in the wastewaters [11].…”

Section: Introductionmentioning

confidence: 99%

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A Cyanobacteria-Based Biofilm System for Advanced Brewery Wastewater Treatment

et al. 2020

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Algal/cyanobacterial biofilm photobioreactors provide an alternative technology to conventional photosynthetic systems for wastewater treatment based on high biomass production and easy biomass harvesting at low cost. This study introduces a novel cyanobacteria-based biofilm photobioreactor and assesses its performance in post-treatment of brewery wastewater and biomass production. Two different supporting materials (glass/polyurethane) were tested to investigate the effect of surface hydrophobicity on biomass attachment and overall reactor performance. The reactor exhibited high removal efficiency (over 65%) of the wastewater’s pollutants (chemical oxygen demand, nitrate, nitrite, ammonium, orthophosphate, and total Kjeldahl nitrogen), while biomass per reactor surface reached 13.1 and 12.8 g·m−2 corresponding to 406 and 392 mg·L−1 for glass and polyurethane, respectively, after 15 days of cultivation. The hydrophilic glass surface favored initial biomass adhesion, although eventually both materials yielded complete biomass attachment, highlighting that cell-to-cell interactions are the dominant adhesion mechanism in mature biofilms. It was also found that the biofilm accumulated up to 61% of its dry weight in carbohydrates at the end of cultivation, thus making the produced biomass a suitable feedstock for bioethanol production.

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Section: Culture Identification and Inoculum Preparationmentioning

confidence: 99%

Section: Biomass Harvesting and Determination Of Intracellular Compoundsmentioning

confidence: 99%

Section: Biomass Harvesting and Determination Of Intracellular Compoundsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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A Cyanobacteria-Based Biofilm System for Advanced Brewery Wastewater Treatment

et al. 2020

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“…was proved effective in the treatment of agro-industrial effluents, such as raisin, winery and dairy wastewaters as well as poplar sawdust and grass hydrolysates, both in suspended and attached cultivation systems [45][46][47]. The same consortium also contained remarkable percentages of carbohydrates that exceeded 40% of dry biomass, thus making it a promising candidate as a substrate for bioethanol production [48].…”

Section: Introductionmentioning

confidence: 98%

Utilization of Biomass Derived from Cyanobacteria-Based Agro-Industrial Wastewater Treatment and Raisin Residue Extract for Bioethanol Production

Tsolcha

Patrinou

Economou

et al. 2021

Water

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Biofuels produced from photosynthetic microorganisms such as microalgae and cyanobacteria could potentially replace fossil fuels as they offer several advantages over fuels produced from lignocellulosic biomass. In this study, energy production potential in the form of bioethanol was examined using different biomasses derived from the growth of a cyanobacteria-based microbial consortium on a chemical medium and on agro-industrial wastewaters (i.e., dairy wastewater, winery wastewater and mixed winery–raisin effluent) supplemented with a raisin residue extract. The possibility of recovering fermentable sugars from a microbial biomass dominated by the filamentous cyanobacterium Leptolynbgya sp. was demonstrated. Of the different acid hydrolysis conditions tested, the best results were obtained with sulfuric acid 2.5 N for 120 min using dried biomass from dairy wastewater and mixed winery–raisin wastewaters. After optimizing sugar release from the microbial biomass by applying acid hydrolysis, alcoholic fermentation was performed using the yeast Saccharomyces cerevisiae. Raisin residue extract was added to the treated biomass broth in all experiments to enhance ethanol production. Results showed that up to 85.9% of the theoretical ethanol yield was achieved, indicating the potential use of cyanobacteria-based biomass in combination with a raisin residue extract as feedstock for bioethanol production.

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Biotreatment of discarded fruit juices and simultaneous lipid production using Leptolyngbya sp.

Patrinou

Αντωνοπούλου

Ντάϊκου³

et al. 2023

J of Chemical Tech & Biotech

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BACKGROUND: Fruit juices are an exceptional source of nutrients; however, due to their short shelf-life and vulnerability to microbial degradation, large volumes of juice are regularly discarded worldwide. As a wastewater, discarded fruit juices (DFJ) possess high organic loads with significant amounts of nitrogen and phosphorus, making them a suitable substrate for microbial growth and to produce natural bioactive compounds.RESULTS: Discarded fruit juices (DFJ) were treated and simultaneously valorized (producing microbial lipids) using a microbial consortium dominated by the photosynthetic cyanobacterium Leptolyngbya sp. The effects of N:P and C:N ratios on growth and lipid production were examined, along with the ability of the consortium to biotreat DFJ in laboratory-scale (LS) reactors. Using the optimum nutrient ratios (N:P 5:1, C:N 50.2:1), scaled-up reactors (aquariums) were then tested under suspended and attached growth conditions. The biomass produced was further exploited for lipid production and the results revealed that Leptolyngbya sp. was able to produce up to 19% d.w. lipids and simultaneously efficiently treat DFJ. High dissolved-chemical oxygen demand (d-COD), total nitrogen, and PO 4 3− removal rates (85.0% and 93.9%, 93.4% and 95.2%, 84.6% and 83.9%, respectively) were achieved in both the suspended and attached growth systems, along with high biomass productivities (316.3, 340.0 mg L −1 day −1 , respectively). CONCLUSION: The biological approach used in this study demonstrated that is possible to exploit DFJ and achieve satisfactory lipid contents (up to 19% d.w.) using a cyanobacterial-based consortium.

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Brewery wastewater treatment using cyanobacterial-bacterial settleable aggregates

Cited by 36 publications

References 63 publications

A Cyanobacteria-Based Biofilm System for Advanced Brewery Wastewater Treatment

A Cyanobacteria-Based Biofilm System for Advanced Brewery Wastewater Treatment

Utilization of Biomass Derived from Cyanobacteria-Based Agro-Industrial Wastewater Treatment and Raisin Residue Extract for Bioethanol Production

Biotreatment of discarded fruit juices and simultaneous lipid production using Leptolyngbya sp.

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