Brevundimonas denitrificans sp. nov., a denitrifying bacterium isolated from deep subseafloor sediment

Abstract

A novel Gram-stain-negative, aerobic, heterotrophic, stalked and capsulated bacterium with potential denitrification ability, designated strain TAR-002 T , was isolated from deep seafloor sediment in Japan. Colonies lacked lustre, and were viscous and translucent white. The ranges of temperature, pH and salt concentration for growth were 8-30 6C, pH 6.0-10.0 and 1-3 % (w/v) NaCl. Phylogenetic analysis based on 16S rRNA gene sequences confirmed that strain TAR-002 T belongs to the genus Brevundimonas of the class Alphaproteobacteria. Levels of similarity between the 16S rRNA gene sequence of strain TAR-002 T and those of the type strains of species of the genus Brevundimonas were 93.5-98.9 %; the most closely related species was Brevundimonas basaltis. In DNA-DNA hybridization assays between strain TAR-002 T and its phylogenetic neighbours, Brevundimonas lenta DS-18 T , B. basaltis J22 T , Brevundimonas subvibrioides ATCC 15264 T and Brevundimonas alba DSM 4736 T , mean hybridization levels were 6.4-27.7 %. The G+C content of strain TAR-002 T was 70.3 mol%. Q-10 was the major respiratory isoprenoid quinone. The major fatty acids were C 18 : 1 v7c and C 16 : 0 , and the presence of 1,indicates the affiliation of strain TAR-002 T with the genus Brevundimonas. On the basis of biological characteristics and 16S rRNA gene sequence comparisons, strain TAR-002 T is considered to represent a novel species of the genus Brevundimonas, for which the name Brevundimonas denitrificans sp. nov. is proposed; the type strain is TAR-002 T (5NBRC 110107 T 5CECT 8537 T ).

Biosurfactant Production in Sub-Oxic Conditions Detected in Hydrocarbon-Degrading Isolates from Marine and Estuarine Sediments

“…All deep-sea isolates identified in this study are represented in the classification of operational taxonomic units from pyrosequencing results [76] and corresponded to genera that were previously reported in marine sediments [55,70,[77][78][79][80][81]. The fact that isolates retrieved from selective cultures of MV sediments are associated with the degradation of PHs is consistent with previous findings, in which communities from inactive MVs presented a preference for degradation of PAHs, instead of simpler PHs [76].…”

Effect of tributyrin supplementation in high‐soya bean meal diet on growth performance, body composition, intestine morphology and microbiota of juvenile yellow drum (Nibea albiflora)

“…By contrast, Acinetobacter relative abundance was below 1% in fish fed the FM and TB0.1 diets, while Brevundimonas was the predominant genera. Brevundimonas strains are usually been isolated from soil (Yoon, Kang, Oh, Lee, & Oh, 2006), sand (Yoon et al, 2006) and sub-seafloor sediment (Tsubouchi et al, 2014), but rarely from infections (Estrela & Abraham, 2010). These changes in predominant or 'core' microbiota induced by tributyrin supplementation may provide evidence for the improvement of intestine health.…”

Electrical Retrieval of Living Microorganisms from Cryopreserved Marine Sponges Using a Potential-Controlled Electrode

“…The ER method is a powerful tool to eliminate unnecessary microbiota and obtain rare environmental microorganisms by potential application of optimal wave shape, optimal resonance frequency, optimal amplitude, and optimal time length. We reduced unwanted microbiota and isolated the novel gram-negative strain Brevundimonas denifitricans from 1180-m deep-seafloor sediment 11 m below the seabed off the Shimokita Peninsula, Japan, using the ER method (Tsubouchi et al 2014 ). We reported that microorganisms such as E. coli recognized and selectively attached to small regions of a −0.4-V vs. Ag/AgCl applied potential 5-μmϕ circular ITO microelectrode array at the single-cell level (Koyama et al 2013 ).…”