Brevinin-1GHd: a novel <i>Hylarana guentheri</i> skin secretion-derived Brevinin-1 type peptide with antimicrobial and anticancer therapeutic potential

Jiang, Yangyang; Wu, Yue; Wang, Tao; Chen, Xiaoling; Ma, Chengbang; Xi, Xinping; Zhang, Ying; Shaw, Christopher; Wang, Lei

doi:10.1042/bsr20200019

Cited by 24 publications

(20 citation statements)

References 27 publications

(37 reference statements)

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“…Dermaseptin-PS1 inhibits human glioblastoma U-251 MG cells (from cells frozen in Uppsala in 1969) via inducing apoptosis at a low micromolar concentration and disrupts cancer cell membranes at 10-fold the concentration [ 112 ]. Moreover, brevinin-1GHd was shown to inhibit proliferation of human cancer lines [ 113 ]. As a means to improve cell selectivity of anticancer peptides, some compounds may be utilized to potentiate peptide potency [ 114 ].…”

Section: Resultsmentioning

confidence: 99%

Bioinformatic Analysis of 1000 Amphibian Antimicrobial Peptides Uncovers Multiple Length-Dependent Correlations for Peptide Design and Prediction

Wang

2020

Antibiotics

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Amphibians are widely distributed on different continents, except for the polar regions. They are important sources for the isolation, purification and characterization of natural compounds, including peptides with various functions. Innate immune antimicrobial peptides (AMPs) play a critical role in warding off invading pathogens, such as bacteria, fungi, parasites, and viruses. They may also have other biological functions such as endotoxin neutralization, chemotaxis, anti-inflammation, and wound healing. This article documents a bioinformatic analysis of over 1000 amphibian antimicrobial peptides registered in the Antimicrobial Peptide Database (APD) in the past 18 years. These anuran peptides were discovered in Africa, Asia, Australia, Europe, and America from 1985 to 2019. Genomic and peptidomic studies accelerated the discovery pace and underscored the necessity in establishing criteria for peptide entry into the APD. A total of 99.9% of the anuran antimicrobial peptides are less than 50 amino acids with an average length of 24 and a net charge of +2.5. Interestingly, the various amphibian peptide families (e.g., temporins, brevinins, esculentins) can be connected through multiple length-dependent relationships. With an increase in length, peptide net charge increases, while the hydrophobic content decreases. In addition, glycine, leucine, lysine, and proline all show linear correlations with peptide length. These correlations improve our understanding of amphibian peptides and may be useful for prediction and design of new linear peptides with potential applications in treating infectious diseases, cancer and diabetes.

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Section: Resultsmentioning

confidence: 99%

Bioinformatic Analysis of 1000 Amphibian Antimicrobial Peptides Uncovers Multiple Length-Dependent Correlations for Peptide Design and Prediction

Wang

2020

Antibiotics

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“…AMPs have been recognized as the first line of defense against pathogens infection, and a growing body of evidence suggested that some AMPs showed anticancer and antiviral activities (Jiang et al 2020 ). In the present study, LHH1 was a novel AMP that identified from the genome of L. casei HZ1 with potent antibacterial effect on S. aureus and anticancer potency on several cancer cells (Table 3 and Fig.…”

Section: Discussionmentioning

confidence: 99%

LHH1, a novel antimicrobial peptide with anti-cancer cell activity identified from Lactobacillus casei HZ1

Jin

Luo

et al. 2020

AMB Expr

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Antimicrobial peptides have been attracting increasing attention for their multiple beneficial effects. In present study, a novel AMP with a molecular weight of 1875.5 Da, was identified from the genome of Lactobacillus casei HZ1. The peptide, which was named as LHH1 was comprised of 16 amino acid residues, and its α-helix content was 95.34% when dissolved in 30 mM SDS. LHH1 exhibited a broad range of antimicrobial activities against Gram-positive bacteria and fungus. It could effectively inhibit Staphylococcus aureus with a minimum inhibitory concentration of 3.5 μM and showed a low hemolytic activity. The scanning electron microscope, confocal laser scanning microscope and flow cytometry results showed that LHH1 exerted its antibacterial activity by damaging the cell membrane of Staphylococcus aureus. Meanwhile, LHH1 also exhibited anti-cancer cell activities against several cancer cells via breaking the cell membrane of MGC803, HCT116 and C666-1 cancer cells.

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“…AMPs have been recognized as the rst line of defence against pathogens infection, and a growing body of evidence suggested that some bactericidal peptides showed anticancer and antiviral activities (Jiang et al 2020). In the present study, LHH1 was a novel AMP that identi ed from the genome of L. casei HZ1 with potent bactericidal effect on S. aureus and anticancer potency on several cancer cells (Table 3 and Fig.…”

Section: Discussionmentioning

confidence: 65%

LHH1, a Novel Antimicrobial Peptide with Anti-Cancer Activity Identified from Lactobacillus casei HZ1

Jin

Luo

et al. 2020

Preprint

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Antimicrobial peptides have been attracting increasing attention for their multiple beneficial effects. In present study, a novel AMP with a molecular weight of 1875.25 Da, was identified from the genome of Lactobacillus casei HZ1. The peptide, which was named as LHH1 was comprised of 16 amino acid residues, and its α-helix content was 95.34% when dissolved in 30 mM SDS. LHH1 exhibited a broad range of antimicrobial activities against gram-positive bacteria and fungus. It could effectively inhibit staphylococcus aureus with a minimum inhibitory concentration of 3.5 μM and showed a low hemolytic activity. The scanning electron microscope, confocal laser scanning microscope and flow cytometry results showed that LHH1 exerted its antibacterial activity by damaging the cell membrane of staphylococcus aureus. Meanwhile, LHH1 also exhibited anti-cancer activities against several cancer cells including MGC803, HCT116 and C666-1 cells, and one major action of its anti-cancer mechanism was breaking the cell membrane.

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Brevinin-1GHd: a novel Hylarana guentheri skin secretion-derived Brevinin-1 type peptide with antimicrobial and anticancer therapeutic potential

Cited by 24 publications

References 27 publications

Bioinformatic Analysis of 1000 Amphibian Antimicrobial Peptides Uncovers Multiple Length-Dependent Correlations for Peptide Design and Prediction

Bioinformatic Analysis of 1000 Amphibian Antimicrobial Peptides Uncovers Multiple Length-Dependent Correlations for Peptide Design and Prediction

LHH1, a novel antimicrobial peptide with anti-cancer cell activity identified from Lactobacillus casei HZ1

LHH1, a Novel Antimicrobial Peptide with Anti-Cancer Activity Identified from Lactobacillus casei HZ1

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