Brevibacterium litoralis sp. nov., isolated from marine surface sediment

Abstract

A Gram stain-positive, non-spore-forming, non-motile, short-rod actinomyces strain GXQ1321T was isolated from maritime surface sediments in Beihai, Guangxi Zhuang Autonomous Region, and a number of categorization studies were performed. Following a period of 72 hours of incubation at a temperature of 30°C within an actinomycetes culture medium, the colony was yellow, circular, smooth, central bulge, convex, opaque, with a 1.8-3.0 mm diameter. Chemotaxonomic studies revealed that the major menaquinone in strain GXQ1321T is MK-8. The most prevalent cellular fatty acids were anteiso-C19:0 (27.28%), anteiso-C15:0 (18.97%), anteiso-C17:0 (15.95%), and iso-C16:0 (12.21%). The whole-cell sugars of the strain GXQ1321T identified were rhamnose, xylose and glucose. Strain GXQ1321T exhibited the presence of meso-diaminopimelic acid (m-DAP) as a distinctive cell-wall diamino acid, and the polar lipids were identified as diphosphatidylglycerol (DPG), three phosphoglycolipidsone, phosphatidylethanolamine (PE), one unknown phospholipid (UP) and one unknown glycolipid (UG). This strain had 69.6% DNA G + C content. Strain GXQ1321T is classified as Brevibacterium based on its 16S rRNA gene sequence. It is closely related to Brevibacterium samyangense SST-8T (96.77%) and Brevibacterium rongguiense 5221T (96.32%). The results showed that the average nucleotide identity (ANI) values of GXQ1321T and the above two strain tyoes were 73.91–77.14%, and the digital DNA-DNA hybridisation (dDDH) values were 15.3–21.1%. Based on the phylogenetic, chemotaxonomi and physiologicalc data, strain GXQ1321T was considered to be a new species of the genus Brevibacterium, named Brevibacterium litoralis sp. nov, with the type strain GXQ1321T (= MCCC 1K08964T = KCTC 59167T).