Breeding Roses for Disease Resistance

Whitaker, Vance M.; Hokanson, Stan C.

doi:10.1002/9780470593783.ch6

Cited by 5 publications

(8 citation statements)

References 106 publications

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“…Finding a significant, negative correlation (r = -0.642; P < 0.01) between our laboratory-based LL data and overall landscape performance ratings in a 3-year evaluation of the roses that eventually earned south-central U.S. Earth-Kind designation highlights the strong impact of black spot resistance on overall landscape performance of roses (Mackay et al, 2008). Another approach to developing durably resistant cultivars would be for breeders to continue to identify race-specific black spot resistance genes and pyramid them into single cultivars (Whitaker and Hokanson, 2009b). To date, three race-specific resistance genes have been identified (Whitaker et al, 2010a).…”

Section: Factorsmentioning

confidence: 85%

“…For each cultivar · race combination assessed at an inoculation date, two boxes of leaves were prepared. 'Chorale' was the universal susceptible control cultivar for all inoculations except for 9 Sept. 2008 when it was not available in sufficient quantity and 'Pariser Charme', also susceptible to all three races (Whitaker and Hokanson, 2009b), was substituted. Conidial concentrations of the inoculum varied with availability on each date and ranged between 30,000 and 100,000 conidia/ mL.…”

Section: Methodsmentioning

confidence: 99%

“…It has been noted that some cultivars defoliated under lower disease pressure than others, an observation that has been confirmed by showing that LL is not necessarily related to frequency of defoliation (Whitaker et al, 2007b). A deficiency of the detached leaf inoculation method is its inability to measure the defoliation response, which can be influenced by rose cultivar response to ethylene and other factors (Whitaker and Hokanson, 2009b).…”

Section: Factorsmentioning

confidence: 99%

“…However, until a singlespore isolate is shown to infect such a rose, race-specific resistance cannot be differentiated from the very unlikely event of universal resistance. Race-specific resistance has recently been documented for three of the nine cultivars resistant to Races 3, 8, and 9, because infected plants have been identified in landscapes (Brite Eyesä and Home Run Ò , unpublished data; Knock Out Ò , Whitaker and Hokanson, 2009b). Finding resistance-breaking pathogen isolates on cultivars characterized as resistant to all previously known races is a valuable tool in the identification of new races.…”

Section: Factorsmentioning

confidence: 99%

“…Detached leaf assays have been an efficient tool to characterize the resistance of rose seedling populations, and they have been found to be strongly correlated with whole plant inoculations (Hattendorf et al, 2004;Von Malek and Debener, 1998;Whitaker and Hokanson, 2009a). Detached leaf assays are preferable as a result of greater ease in controlling humidity and inoculum levels (Whitaker and Hokanson, 2009b). To the best of our knowledge, single-spore isolates of D. rosae have only been used for race characterization, comparison of pathogenicity of isolates, and to study the segregation of resistance to particular races in genetic studies and the characterization of partial resistance components.…”

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confidence: 99%

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Evaluation of Roses from the Earth-Kind® Trials: Black Spot (Diplocarpon rosae Wolf) Resistance and Ploidy

Zlesak¹,

Whitaker²,

George³

et al. 2010

horts

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Additional index words. rose chromosome count, rose disease, fungal isolate, Diplocarpon rosae pathogenic race Abstract. Regional, replicated cultivar trials of landscape roses are an ongoing component of the Earth-Kind Ò program, which was started at Texas A&M University in the 1990s to support environmental landscape stewardship. The rose trials within the Earth-Kind program identify and promote the most regionally adapted rose cultivars and are conducted without fertilizers or pesticides and greatly reduced irrigation. Black spot (caused by Diplocarpon rosae Wolf) is the most serious disease of outdoor-grown roses worldwide as a result of the potential for rapid leaf yellowing and defoliation. Earth-Kind designated cultivars for the south-central United States and roses under trial in other regions or considered for future Earth-Kind trials (n = 73 roses) and two susceptible control cultivars were challenged with North American Races 3, 8, and 9 of D. rosae, which were previously characterized at the University of Minnesota. Young expanded leaves were inoculated using detached leaf assays. Lesion length (LL) was measured for susceptible reactions and cultivar ploidy was determined using root tip squashes. Diploid, triploid, and tetraploid cultivars (n = 20, 30, and 23, respectively) were identified, and race-specific resistances and partial resistances were also identified. Race-specific resistance was generally more prevalent in newer rose cultivars and rose cultivars more recently included in Earth-Kind trials. Nine cultivars were resistant to all three races (Brite Eyesä, 'Grouse', Home Run Ò , Knock Out Ò , Paprikaä, Peachy Creamä, Pink Knock Out Ò , Rainbow Knock Out Ò , and Yellow Submarineä). Blushing Knock Out Ò , a sport of Knock Out Ò , was susceptible to Race 8. Partial resistance rank for LL was generally consistent across races for roses susceptible to multiple races. The application of these data includes: characterizing the minimum resistance level needed for roses to warrant inclusion in Earth-Kind field trials, the identification of additional race-specific resistance genes, identifying resistance-breaking isolates of D. rosae, understanding race composition in field trials based on infection patterns of key cultivars, selection of parents for resistance breeding efforts, and continued comparisons between LL and growing bodies of Earth-Kind field resistance data.

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Section: Factorsmentioning

confidence: 85%

Section: Methodsmentioning

confidence: 99%

Section: Factorsmentioning

confidence: 99%

Section: Factorsmentioning

confidence: 99%

mentioning

confidence: 99%

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Evaluation of Roses from the Earth-Kind® Trials: Black Spot (Diplocarpon rosae Wolf) Resistance and Ploidy

Zlesak¹,

Whitaker²,

George³

et al. 2010

horts

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Rdr3, a novel locus conferring black spot disease resistance in tetraploid rose: genetic analysis, LRR profiling, and SCAR marker development

et al. 2009

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Black spot disease of rose, incited by the fungus Diplocarpon rosae, is found worldwide and is the most important disease of garden roses. A gene-for-gene interaction in this pathosystem is evidenced by the presence of pathogenic races of D. rosae and the previous discovery of a dominant resistance allele at the Rdr1 locus in the diploid Rosa multiflora. The objective of the present study was to genetically analyze resistances to North American black spot races 3, 8, and 9 previously reported in tetraploid roses. Resistance to North American races 3 and 8 segregated 1:1 in multiple F(1) populations, indicating that both are conferred by dominant alleles at single loci and are present in simplex (Rrrr) configuration. Gene pyramiding was demonstrated by combining both resistances into single genotypes. Resistance to race 9 was partial and segregated in a quantitative fashion. Analysis of these populations with microsatellite markers previously developed for Rdr1 revealed that the gene conferring race 3 resistance resides within the same R gene cluster as Rdr1. Race 8 resistance segregated independently and is, therefore, a novel locus for black spot resistance in rose which we have named Rdr3. NBS and LRR profiling were used in a bulked segregant analysis to identify a marker 9.1 cM from Rdr3, which was converted to a SCAR marker form for marker-assisted breeding.

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Transcriptome changes in resistant and susceptible rose in response to powdery mildew

Chandran

Sriram

Prakash

et al. 2021

Journal of Phytopathology

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Rose is one of the most important ornamental crops worldwide and powdery mildew (Podosphaera pannosa (Wallr.: Fr.) de Bary) is the major disease of rose when grown in polyhouse or open field. Understanding of plant–pathogen interaction is important to develop strategies for disease management. We profiled transcriptomes of powdery mildew‐resistant rose line IIHRR13‐4 and susceptible cultivar Konfetti using Illumina NGS platform to understand the molecular mechanism of resistance to powdery mildew in the rose line IIHRR13‐4. Profiles of samples were collected at 0 hr after inoculation (hai), 36 hai and 72 hai from susceptible cultivar Konfetti (SCON0, SCON3 and SCON7) and 0 hai and 36 hai from resistant line IIHRR13‐4 (RPMR0 and RPMR3). The high‐quality reads were aligned to reference genome of Rosa chinensis. The transcripts of rose‐powdery mildew interaction were analysed for functions and involvement in disease resistance. Expression of receptor‐like kinases, putative disease resistance genes (RLKs, NBS‐LRR, NB‐ARC, RPP13), WRKY transcription factors and PR proteins was observed in the transcripts library and was found up‐regulated in IIHRR13‐4 samples. Differentially expressed genes coding for metabolic and cellular process, response to abiotic stress response, defence response and other biosynthetic processes were identified in the transcripts. Gene ontology term enrichment and pathway analysis revealed that transcriptome of IIHRR13‐4 was enriched with transcripts coding for plant hormone signal transduction and plant–pathogen interaction compared to transcripts of susceptible cultivar Konfetti. The transcriptome profiling proved that different defence mechanisms including hypersensitive responses were elicited as systemic acquired resistance in rose line IIHRR13‐4 when infected by powdery mildew pathogen.

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Breeding Roses for Disease Resistance

Cited by 5 publications

References 106 publications

Evaluation of Roses from the Earth-Kind® Trials: Black Spot (Diplocarpon rosae Wolf) Resistance and Ploidy

Evaluation of Roses from the Earth-Kind® Trials: Black Spot (Diplocarpon rosae Wolf) Resistance and Ploidy

Rdr3, a novel locus conferring black spot disease resistance in tetraploid rose: genetic analysis, LRR profiling, and SCAR marker development

Transcriptome changes in resistant and susceptible rose in response to powdery mildew

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