Breeding prospects of Lunaria annua L.

Mastebroek, H.D.; Marvin, H.J.P.

doi:10.1016/s0926-6690(99)00056-4

Cited by 26 publications

(22 citation statements)

References 2 publications

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“…A biennial or perennial cruciferous plant, it has a high (35%) oil content in its seeds. The oil, comprised of very long-chain mono-unsaturated fatty acids (e.g., nervonic acid) has been used as an industrial lubricant (Guo et al, 2009;Mastebroek and Marvin, 2000) and may have potential interest for other applications, e.g. for biofuel production like other Brassicaceae (Appelquist, 1976;Ciccarelli et al, 2010;Papini and Simeone, 2010;Papini et al, 2010a,b).…”

Section: Introductionmentioning

confidence: 99%

The unusual tegumental tissues of the Lunaria annua (Brassicaceae) seed: A developmental study using light and electron microscopy

Mosti

Friedman

Piccolin

et al. 2012

Flora - Morphology, Distribution, Functional Ecology of Plants

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Please cite this article in press as: Mosti, S., et al., The unusual tegumental tissues of the Lunaria annua (Brassicaceae) seed: A developmental study using light and electron microscopy. Flora (2012) With light, fluorescence, transmission electron, and environmental scanning electron microscopy we studied the development of the Lunaria annua L. (Brassicaceae) seeds in order to reveal basic anatomical information about the unusual tissues of these seeds. In particular the seed tegument tissues possess complex morphological aspects that are relevant to the biology and ecology of this plant. A sclerenchymatic tissue as the innermost layer of the teguments apparently offers robust protection for the embryo, yet is organized to be flexible. This tissue likely controls the passage of water from the tegumental layers towards the embryo. We report here the presence of tannins in the pre-sclerenchymatic layer of the unripe seed. The inner tegument also houses a spongy tissue with wide intercellular spaces. This tissue could impart buoyancy to the seeds, which possibly might be required for water transport. The structural features could indicate that Lunaria may have evolved in a Mediterranean environment, which is characterized by a long dry season, but with a large amount of rainfall concentrated in short periods. Probably, not only the typical enlarged and flattened fruits of Lunaria can easily float and be dispersed away from the mother plant, but also the seeds have this dispersal peculiarity after release from the silicules.

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Section: Introductionmentioning

confidence: 99%

The unusual tegumental tissues of the Lunaria annua (Brassicaceae) seed: A developmental study using light and electron microscopy

Mosti

Friedman

Piccolin

et al. 2012

Flora - Morphology, Distribution, Functional Ecology of Plants

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“…Unmodified Lunaria annua oil has been used on a small scale as an industrial lubricant in the past (Meier zu Beerentrup and Röbbelen 1987;Van Soest 1994). Lunaria annua seeds contain oil which consists of 65% VLCMFAs (44% erucic acid (22:1D13), and 20% nervonic acid (24:1; cis-tetracos-15-enoic acid); Mastebroek and Marvin 2000). Seed yields vary widely, between 800 and 2,000 kg/ha, and with an oil content of approximately 30%, yields of nervonic-enriched oil are too low for a crop that needs a growing period of 2 years before harvest.…”

Section: Introductionmentioning

confidence: 99%

“…Seed shattering is also a problem (Nicholls 1996). Thus, it is uneconomical to grow L. annua as a major source of nervonic acid, even with set-aside payments (Mastebroek and Marvin 2000).…”

Section: Introductionmentioning

confidence: 99%

Increase in nervonic acid content in transformed yeast and transgenic plants by introduction of a Lunaria annua L. 3-ketoacyl-CoA synthase (KCS) gene

et al. 2008

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Nervonic acid is a Very Long-Chain Monounsaturated Fatty Acid (VLCMFA), 24:1 Delta15 (cis-tetracos-15-enoic acid) found in the seed oils of Lunaria annua, borage, hemp, Acer (Purpleblow maple) and Tropaeolum speciosum (Flame flower). However, of these, only the "money plant" (Lunaria annua L.) has been studied and grown sparingly for future development as a niche crop and the outlook has been disappointing. Therefore, our goal was to isolate and characterize strategic new genes for high nervonic acid production in Brassica oilseed crops. To this end, we have isolated a VLCMFA-utilizing 3-Keto-Acyl-CoA Synthase (KCS; fatty acid elongase; EC 2.3.1.86) gene from Lunaria annua and functionally expressed it in yeast, with the recombinant KCS protein able to catalyze the synthesis of several VLCMFAs, including nervonic acid. Seed-specific expression of the Lunaria KCS in Arabidopsis resulted in a 30-fold increase in nervonic acid proportions in seed oils, compared to the very low quantities found in the wild-type. Similar transgenic experiments using B. carinata as the host resulted in a 7-10 fold increase in seed oil nervonic acid proportions. KCS enzyme activity assays indicated that upon using (14)C-22:1-CoA as substrate, the KCS activity from developing seeds of transgenic B. carinata was 20-30-fold higher than the low erucoyl-elongation activity exhibited by wild type control plants. There was a very good correlation between the Lun KCS transcript intensity and the resultant 22:1-CoA KCS activity in developing seed. The highest nervonic acid level in transgenic B. carinata expressing the Lunaria KCS reached 30%, compared to 2.8% in wild type plant. In addition, the erucic acid proportions in these transgenic lines were considerably lower than that found in native Lunaria oil. These results show the functional utility of the Lunaria KCS in engineering new sources of high nervonate/reduced erucic oils in the Brassicaceae.

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“…3.10) is commonly known as the honesty or money plant because of the shape of its seed pods. Lunaria annua is a plant native to southern Europe and western Asia, is well adapted to many temperate countries of Europe and North America, was introduced in northern Europe several centuries ago, and is widely grown as an ornamental fl owering plant or as a wildfl ower in gardens and in wastelands (Smith et al 1997;Walker, Walker, and Booth 2003;Cromack 1998;Masterbroek and Marvin 2000).…”

Section: Lunaria (Lunaria Annua L Brassicaciae/crusiferae)mentioning

confidence: 99%

“…3.1), and thus they can be used by the oleochemical industry for the production of high-temperature lubricants, erucamides, additives, and pharmaceutical products (Smith et al 1997;Walker, Walker, and Booth 2003;Cromack 1998;Masterbroek and Marvin 2000;IENICA 2002d). Lunaria is still at the developmental stage.…”

Section: Lunaria (Lunaria Annua L Brassicaciae/crusiferae)mentioning

confidence: 99%

3 The terrestrial biomass: formation and properties (crops and residual biomass)

Christou¹,

Alexopoulou²

2012

Biorefinery

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Breeding prospects of Lunaria annua L.

Cited by 26 publications

References 2 publications

The unusual tegumental tissues of the Lunaria annua (Brassicaceae) seed: A developmental study using light and electron microscopy

The unusual tegumental tissues of the Lunaria annua (Brassicaceae) seed: A developmental study using light and electron microscopy

Increase in nervonic acid content in transformed yeast and transgenic plants by introduction of a Lunaria annua L. 3-ketoacyl-CoA synthase (KCS) gene

3 The terrestrial biomass: formation and properties (crops and residual biomass)

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