Breeding of Xylanase-Producing Strains by Ion Implantation and Studying of Fermentation Conditions

Abstract

With ion implantation, a high xylanase-producing strainAspergillus nigerBU99 was selected. Different late acting carbon sources (wheat bran, wheat straw, corncob and bagasse) had an effect on enzymatic production. Additionally, it was found that fast acting carbon sources (lactose, galactose, sucrose, glucose and maltose) put into the media affected on xylanase production too. Severe catabolic repression was observed in the media with lactose, but other sugars (galactose, sucrose, glucose and maltose) could increase the production of xylanase compared with the control. The production of xylanase was improved too when corncob flour was hydrolyzed by α-amylase, cellulase or/and glucoamylase. Additionally, it was found that the production of xylanase would be almost higher at 60h when the media contained glucose or were hydrolyzed by enzymes.

The Research on Growthtraits of Alfalfa Irradiated by ¹²C^{6 +} Ion Beam

“…Wang Songli uses 25Kev nitrogen ion beam as mutation source, and screens rice plants which chlorophyll content are 148.67% higher than control plants [1] . Tobacco Institute of Agricultural Sciences Academy of Henan Province has made a research, 8 strains of tobacco seeds were treated by ion beams, screened a number of strong disease resistance strain [ 2] . In recent years, heavy ion is emerging as a radiation source, due to its unique advantages in the life science research plays an important role in plant breeding, and has achieved great economic and social benefits, providing a new way for plant breeding.…”