Breeding for introgression of FAD2-1A and FAD2-1B genes to local soybean cultivars of Turkey

Ustun, Rustem; Uzun, Bülent

doi:10.1016/j.jbiotec.2017.06.1151

Cited by 2 publications

(3 citation statements)

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“…Previous reports showed that some natural variations in FAD2 orthologs resulted in an elevated C18:1/C18:2 ratio in oil seed crops with C18:2 as the major fatty acid [10,11,14,23,24,25]. Thus it is feasible to find some natural FAD2 dysfunction variants in Idesia ploycarpa .…”

Section: Resultsmentioning

confidence: 99%

A Conserved Glycine Is Identified to be Essential for Desaturase Activity of IpFAD2s by Analyzing Natural Variants from Idesia polycarpa

Zhang

Feng

et al. 2018

IJMS

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High amounts of polyunsaturated fatty acids (PUFAs) in vegetable oil are not desirable for biodiesel or food oil due to their lower oxidative stability. The oil from Idesia polycarpa fruit contains 65–80% (mol%) linoleic acid (C18:2). Therefore, development of Idesia polycarpa cultivars with low PUFAs is highly desirable for Idesia polycarpa oil quality. Fatty acid desaturase 2 (FAD2) is the key enzyme converting oleic acid (C18:1) to C18:2. We isolated four FAD2 homologs from the fruit of Idesia polycarpa. Yeast transformed with IpFAD2-1, IpFAD2-2 and IpFAD2-3 can generate appreciable amounts of hexadecadienoic acid (C16:2) and C18:2, which are not present in wild-type yeast cells, revealing that the proteins encoded by these genes have Δ12 desaturase activity. Only trace amounts of C18:2 and little C16:2 were detected in yeast cells transformed with IpFAD2-4, suggesting IpFAD2-4 displays low activity. We also analyzed the activity of several FAD2 natural variants of Idesia polycarpa in yeast and found that a highly conserved Gly376 substitution caused the markedly reduced products catalyzed by IpFAD2-3. This glycine is also essential for the activity of IpFAD2-1 and IpFAD2-2, but its replacement in other plant FAD2 proteins displays different effects on the desaturase activity, suggesting its distinct roles across plant FAD2s proteins.

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Section: Resultsmentioning

confidence: 99%

A Conserved Glycine Is Identified to be Essential for Desaturase Activity of IpFAD2s by Analyzing Natural Variants from Idesia polycarpa

Zhang

Feng

et al. 2018

IJMS

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“…The soybean breeders mainly inhibited the expression of the FAD2 gene family by the RNAi and CRISPR/Cas9 gene editing technologies, and also reported the transfer of FAD2 antisense genes into oil crops through antisense inhibition technology to increase oleic acid content [8]. The antisense FAD2 gene was transferred into rape by the Agrobacterium tumefaciens mediated method and the obtained transgenic plants with the highest content of oleic acid (68.72%), which was 20% higher than that of the control.…”

Section: Discussionmentioning

confidence: 99%

“…The activity of the FAD2 enzyme is the main factor affecting the synthesis of various unsaturated fatty acids in plants, and FAD2 gene is the coding gene of FAD2 enzyme. Therefore, the FAD2 gene is the key gene to control the conversion of oleic to linoleic acid [8]. It directly determines the proportion of fatty acid components and plays an important role in fatty acid metabolism.…”

Section: Introductionmentioning

confidence: 99%

Construction and Functional Analysis of CRISPR/Cas9 Vector of FAD2 Gene Family in Soybean

Xiao¹,

Jin²,

Zhang³

et al. 2022

Phyton

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Soybean oleic acid content is one of the important indexes to evaluate the quality of soybean oil. In the synthesis pathway of soybean fatty acids, the FAD2 gene family is the key gene that regulates the production of linoleic acid from soybean oleic acid. In this study, CRISPR/Cas9 gene editing technology was used to regulate FAD2 gene expression. Firstly, the CRISPR/Cas9 single knockout vectors GmFAD2-1B and GmFAD2-2C and double knockout vectors GmFAD2-2A-3 were constructed. Then, the three vectors were transferred into the recipient soybean variety Jinong 38 by Agrobacterium-mediated cotyledon node transformation, and the mutant plants were obtained. Functional analysis and comparison of the mutant plants of the T2 and T3 generations were carried out. The results showed that there was no significant difference in agronomic traits between the CRISPR/Cas9 single and double knockout vectors and the untransformed CRISPR/Cas9 receptor varieties. The oleic acid content of the plants that knocked out the CRISPR/Cas9 double gene vector was significantly higher than that of the single gene vector.

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Breeding for introgression of FAD2-1A and FAD2-1B genes to local soybean cultivars of Turkey

Cited by 2 publications

References 0 publications

A Conserved Glycine Is Identified to be Essential for Desaturase Activity of IpFAD2s by Analyzing Natural Variants from Idesia polycarpa

A Conserved Glycine Is Identified to be Essential for Desaturase Activity of IpFAD2s by Analyzing Natural Variants from Idesia polycarpa

Construction and Functional Analysis of CRISPR/Cas9 Vector of FAD2 Gene Family in Soybean

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