Breeding Drought-Tolerant Maize (Zea mays) Using Molecular Breeding Tools: Recent Advancements and Future Prospective

Abstract

As a most significant cereal crop, maize provides vital nutritional components to humans and livestock. Drought stress curtails maize growth and yield by impairing several morphological, physiological, and biochemical functions. The rising threats of drought stress significantly affect global food security and increase the ratio of hunger and starvation. The use of molecular breeding techniques has enabled maize researchers to deeply examine the genetic control of drought tolerance and the genetic differences between genotypes to drought stress. Despite the significant progress in molecular genetics, the drought tolerance mechanism is still not fully understood. With the advancements in molecular research, researchers have identified several molecular factors associated with maize tolerance to drought stress. Quantitative trait loci (QTL) mapping and genome-wide association study (GWAS) analysis have led to identifying QTL, and genes linked to drought tolerance in maize that can be further exploited for their possible breeding applications. Transcriptome and transcription factors (TFs) analysis has revealed the documentation of potential genes and protein groups that might be linked to drought tolerance and accelerate the drought breeding program. Genetic engineering has been used to develop transgenic maize cultivars that are resistant to drought stress. Clustered regularly interspaced short palindromic repeats (CRISPR/Cas9) is a new ray of hope to edit the gene of interest to enhance drought tolerance in maize and save both time and cost in cultivar development. In the current review article, we have tried to present an updated picture of the advancements of drought tolerance in maize and its future prospects. These organized pieces of information can assist future researchers in understanding the basis of drought tolerance to adopt a potential breeding tool for breeding drought-tolerant maize cultivars.

Regulation of gene expression in plant growth and metabolism: A review

“…The up-regulated gene ZmVPP1 in the tolerant genotype of maize was reported at photosynthetic and root-growing stages. Out of 355 genes discovered from the transgenic lines, ZmVPP1 showed significantly altered expression in inbred lines of maize grown under drought conditions [13] . ZmVPP1 encoded a vacuolar-type H(+) pyrophosphatase, which contributes to drought tolerance in maize by boosting photosynthetic efficiency and root development [30] .…”