Breed differences in humoral and cellular responses of lambs to experimental infection with the gastrointestinal nematode Teladorsagia circumcincta

Ahmed, Albin M.; Sebastiano, Simone Rocco; Sweeney, T.; Hanrahan, J. P.; Glynn, Assumpta; Keane, Orla M.; Mukhopadhya, Anindya; Thornton, Kevin; Good, Barbara

doi:10.1186/s13567-014-0137-0

Cited by 15 publications

(15 citation statements)

References 48 publications

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“…The lambs described here were part of a larger experiment concerning the response of Suffolk and Texel lambs to experimental infection with Teladorsagia circumcincta [ 15 ]. Lambs (10 in total; 6 Texel and 4 Suffolk) were born (28th and 29th March) indoors, and moved to pasture until 6 weeks of age (mid-May), at which point they were weaned and moved and kept indoors until mid-September.…”

Section: Methodsmentioning

confidence: 99%

Variation in the Ovine Abomasal Lymph Node Transcriptome between Breeds Known to Differ in Resistance to the Gastrointestinal Nematode

et al. 2015

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Texel lambs are known to be more resistant to gastrointestinal nematode (GIN) infection than Suffolk lambs, with a greater ability to limit infection. The objectives of this study were to: 1) profile the whole transcriptome of abomasal lymph node tissue of GIN-free Texel and Suffolk lambs; 2) identify differentially expressed genes and characterize the immune-related biological pathways and networks associated with these genes. Abomasal lymph nodes were collected from Texel (n = 6) and Suffolk (n = 4) lambs aged 19 weeks that had been GIN-free since 6 weeks of age. Whole transcriptome profiling was performed using RNA-seq on the Illumina platform. At the time of conducting this study, a well annotated Ovine genome was not available and hence the sequence reads were aligned with the Bovine (UMD3.1) genome. Identification of differentially expressed genes was followed by pathway and network analysis. The Suffolk breed accounted for significantly more of the differentially expressed genes, (276 more highly expressed in Suffolk v 162 in Texel; P < 0.001). The four most significant differentially expressed pathways were all related to immunity and were classified as: Role of Pattern Recognition Receptors in Recognition of Bacteria and Viruses, Activation of IRF by Cytosolic Pattern Recognition Receptors, Role of RIG-I-like Receptors in Antiviral Innate Immunity, and Interferon Signaling. Of significance is the fact that all of these four pathways were more highly expressed in the Suffolk. These data suggest that in a GIN-free environment, Suffolk lambs have a more active immune profile relative to the Texel: this immune profile may contribute to the poorer efficiency of response to a GIN challenge in the Suffolk breed compared to the Texel breed.

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Section: Methodsmentioning

confidence: 99%

Variation in the Ovine Abomasal Lymph Node Transcriptome between Breeds Known to Differ in Resistance to the Gastrointestinal Nematode

et al. 2015

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“…Immunoglobulin A was quantified according to the procedure described by Ahmed et al (2015). Briefly, ileal samples were unfrozen overnight at 4 ºC; then the surface was scraped with a scalpel and 2 g were collected in a falcon tube with 6 mL of ice cold phosphate-buffered saline solution supplemented with protease inhibitors (Sigma-Aldrich Corp., Saint Louis, Missouri).…”

Section: Ileal Mucosa Antibody Recovery and Immunoglobulin A Quantifimentioning

confidence: 99%

Early feed restriction of lambs modifies ileal epimural microbiota and affects immunity parameters during the fattening period

Frutos

Andrés

Yáñez-Ruíz

et al. 2018

Animal

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Bacteria firmly attached to the gastrointestinal epithelium during the pre-weaning phase may show a significant impact on nutrient processing, immunity parameters, health and feed efficiency of lambs during post-weaning phases. Thus, the aim of this study was to describe the differences in the ileal epimural microbiota (e.g. total bacteria, Prevotella spp., Bifidobacterium spp. and Lactobacillus spp.) of fattening lambs promoted by early feed restriction during the suckling phase trying to elucidate some of the underlying mechanisms behind changes in feed efficiency during the fattening period. A total of 24 Merino lambs (average BW 4.81±0.256 kg) were used, 12 of them (ad libitum, ADL) kept permanently in individual pens with their mothers, whereas the other 12 lambs were separated from their dams for 9 h each day to be exposed to milk restriction (RES). After weaning (BW=15 kg) all the animals were penned individually, offered the same complete pelleted diet (35 g/kg BW per day) and slaughtered at a BW of 27 kg. During the fattening period, reduced gain : feed ratio (0.320 v. 0.261, P<0.001) was observed for the RES group. Moreover, increments of Prevotella spp. were detected in the ileal epimural microbiota of RES lambs (P<0.05). There were also higher numbers of infiltrated lymphocytes (T and B cells) in the ileal lamina propria (P<0.05), a higher M-cell labelling intensity in ileal Peyer's patches domes (P<0.05) and a trend towards a thickening of the submucosa layer when compared with the ADL group (P=0.057). Some other immunological parameters, such as an increased immunoglobulin A (IgA) production (pg IgA/µg total protein) and increments in CD45+ cells were also observed in the ileum of RES group (P<0.05), whereas transforming growth factor β and toll-like receptor gene expression was reduced (P<0.05). In conclusion, early feed restriction during the suckling phase promoted changes in ileal epimural microbiota and several immunity parameters that could be related to differences in feed efficiency traits during the fattening period of Merino lambs.

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“…However, the relationship between peripheral blood eosinophilia and tissue eosinophilia is reasonably weak, with only a proportion of circulating eosinophils moving into the abomasal mucosa in response to GIN infection . Increases in tissue eosinophils have been observed during H. contortus infection of both naïve and previously sensitized sheep, resistant Romney selection line animals with a naturally acquired mixed infection and Scottish Blackface, Suffolk and Texel lambs infected with T. circumcincta .…”

Section: The Innate Immune Responsementioning

confidence: 99%

The host immune response to gastrointestinal nematode infection in sheep

McRae

Stear

Good

et al. 2015

Parasite Immunology

175

149

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SummaryGastrointestinal nematode infection represents a major threat to the health, welfare and productivity of sheep populations worldwide. Infected lambs have a reduced ability to absorb nutrients from the gastrointestinal tract, resulting in morbidity and occasional mortality. The current chemo‐dominant approach to nematode control is considered unsustainable due to the increasing incidence of anthelmintic resistance. In addition, there is growing consumer demand for food products from animals not subjected to chemical treatment. Future mechanisms of nematode control must rely on alternative, sustainable strategies such as vaccination or selective breeding of resistant animals. Such strategies take advantage of the host's natural immune response to nematodes. The ability to resist gastrointestinal nematode infection is considered to be dependent on the development of a protective acquired immune response, although the precise immune mechanisms involved in initiating this process remain to be fully elucidated. In this study, current knowledge on the innate and acquired host immune response to gastrointestinal nematode infection in sheep and the development of immunity is reviewed.

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Breed differences in humoral and cellular responses of lambs to experimental infection with the gastrointestinal nematode Teladorsagia circumcincta

Cited by 15 publications

References 48 publications

Variation in the Ovine Abomasal Lymph Node Transcriptome between Breeds Known to Differ in Resistance to the Gastrointestinal Nematode

Variation in the Ovine Abomasal Lymph Node Transcriptome between Breeds Known to Differ in Resistance to the Gastrointestinal Nematode

Early feed restriction of lambs modifies ileal epimural microbiota and affects immunity parameters during the fattening period

The host immune response to gastrointestinal nematode infection in sheep

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