Breath sensors for lung cancer diagnosis

Adıgüzel, Yekbun; Külah, Haluk

doi:10.1016/j.bios.2014.10.023

Cited by 147 publications

(103 citation statements)

References 114 publications

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“…[1][2][3][4] VOCs are participants in the reaction of atmospheric photochemistry and can be used as biomarkers containing in the exhaled breath from cancer patients for diagnoses purpose and monitoring the treatments of the diseases. [5][6][7][8] Despite some portable gas sensors are commercially available, such as sensing devices integrated with photoionization detector, they cannot distinguish between different VOCs and the cross reactivity in complex vapor mixtures remains a major challenge in this concise way. 9 So far, electronic nose (e-nose) sensor array which comprises a large number of different sensors coupled with pattern-recognition protocols is one of the most successful platform for VOCs discriminations.…”

Section: Introductionmentioning

confidence: 99%

Detection of Volatile Organic Compounds Using Microfabricated Resonator Array Functionalized with Supramolecular Monolayers

Chang

Tang

et al. 2015

ACS Appl. Mater. Interfaces

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This paper describes the detection of volatile organic compounds (VOCs) using an e-nose type integrated microfabricated sensor array, in which each resonator is coated with different supramolecular monolayers: p-tert-butyl calix[8]arene (Calix[8]arene), 2,3,7,8,12,13,17,18-octaethyl-21H,23H-porphine (Porphyrin), β-cyclodextrin (β-CD), and cucurbit[8]uril (CB[8]). Supramolecular monolayers fabricated by Langmuir-Blodgett techniques work as specific sensing interface for different VOCs recognition which increase the sensor selectivity. Microfabricated ultrahigh working frequency film bulk acoustic resonator (FBAR) transducers (4.4 GHz) enable their high sensitivity toward monolayer gas sensing which facilitate the analyses of VOCs adsorption isotherms and kinetics. Two affinity constants (K1, K2) are obtained for each VOC, which indicate the gas molecule adsorption happen inside and outside of the supramolecular cavities. Additional kinetic information on adsorption and desorption rate constants (ka, kd) are obtained as well from exponential fitting results. The five parameters, one from the conventional frequency shift signals of mass transducers and the other four from the indirect analyses of monolayer adsorption behaviors, thus enrich the sensing matrix (Δf, K1, K2, ka, kd) which can be used as multiparameter fingerprint patterns for highly selective detection and discrimination of VOCs.

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Section: Introductionmentioning

confidence: 99%

Detection of Volatile Organic Compounds Using Microfabricated Resonator Array Functionalized with Supramolecular Monolayers

Chang

Tang

et al. 2015

ACS Appl. Mater. Interfaces

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“…[6][7][8][9][10][11][12][13][14]. Nature and concentration of VOC in the breath extract of healthy subjects differ from that of patients suffering from above mentioned diseases and these differences can to be smelled out by an electronic nose [15][16][17][18][19][20][21].…”

Section: Introductionmentioning

confidence: 99%

Spray layer-by-layer assembly of POSS functionalized CNT quantum chemo-resistive sensors with tuneable selectivity and ppm resolution to VOC biomarkers

Nag

Sachan

Castro

et al. 2016

Sensors and Actuators B: Chemical

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“…Two potential technologies that could meet this need are breath analysis [24] and circulating biomarkers. A number of studies suggest candidate biomarkers, usually panels of tumour proteins [25], [26] or autoantibodies [27], and an associated risk index.…”

Section: Discussionmentioning

confidence: 99%

A quantitative immunoassay for lung cancer biomarker CIZ1b in patient plasma

Coverley

Higgins

West

et al. 2017

Clinical Biochemistry

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ObjectivesNon-invasive tests for early detection of lung cancer are an important unmet clinical need. CIZ1b plasma biomarker can discriminate stage 1 lung cancer from within high-risk groups with clinically useful accuracy, with ROC AUCs in excess of 0.9 for two independent retrospective cohorts, and could therefore meet this need. Our aim was to characterise the native state of the biomarker and develop a quantitative immunoassay.Design and methodsSelective denaturation, preparative electrophoresis and mass spectrometry of human plasma were used to characterise the biomarker and interaction partners. A sandwich ELISA was generated, and specificity for CIZ1b biomarker tested on lung cancer patient plasma.ResultsCIZ1b biomarker is a denaturation-resistant complex between a C-terminal fragment of CIZ1 bearing the CIZ1b epitope specified by alternative splicing of exon14, and fibrinogen alpha chain. Reconstitution of the biomarker epitope with purified fibrinogen and CIZ1b, but not CIZ1a (non-alternatively spliced exon 14) confirmed the specificity of the results. The endogenous complex is highly stable in lung cancer plasma and can be quantified by pairing of a CIZ1b exon-junction specific antibody with detection of fibrinogen. Application of this sandwich ELISA to a prospectively collected development set of plasmas reveals the same level of accuracy as the western blot used to validate the discriminatory capability of the biomarker.ConclusionsUnexpected and unusual molecular structure of CIZ1b in native plasma has complicated immunoassay design, and delayed translation of this promising biomarker. However, CIZ1b can now be measured using a high-throughput, hospital-friendly sandwich ELISA format, overcoming an important barrier to further clinical development and application of this blood test for early stage lung cancer.

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Breath sensors for lung cancer diagnosis

Cited by 147 publications

References 114 publications

Detection of Volatile Organic Compounds Using Microfabricated Resonator Array Functionalized with Supramolecular Monolayers

Detection of Volatile Organic Compounds Using Microfabricated Resonator Array Functionalized with Supramolecular Monolayers

Spray layer-by-layer assembly of POSS functionalized CNT quantum chemo-resistive sensors with tuneable selectivity and ppm resolution to VOC biomarkers

A quantitative immunoassay for lung cancer biomarker CIZ1b in patient plasma

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