Breath metabolomic profiling by nuclear magnetic resonance spectroscopy in asthma

Ibrahim, Baharudin; Marsden, Paul; Smith, Jaclyn A.; Čustović, Adnan; Nilsson, Mathias; Fowler, Stephen J.

doi:10.1111/all.12211

Cited by 46 publications

(46 citation statements)

References 33 publications

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“…Twenty-one studies using metabolomic profiling of exhaled breathe condensate (EBC) (n ¼ 11), [3][4][5][6][7][8][9][10][11][12][13] urine (n ¼ 4), [14][15][16][17] serum (n ¼ 3), [18][19][20] and plasma (n ¼ 3) [21][22][23] were identified (Table 1). Twelve studies evaluated children, [3][4][5][6][7][8][9][10]14,15,21,22 eight evaluated adults, 12,13,[16][17][18][19][20]23 and one included both. 11 The majority used MS-based methods; six used NMR.…”

Section: Methodsmentioning

confidence: 99%

“…11 The majority used MS-based methods; six used NMR. [10][11][12][13]15,20 All but four 16,17,21,22 were case-control in design, and the total number of people with asthma ranged from 10 16 to 343. 9 The primary aim of most studies was to examine the differences between asthma cases and healthy control patients, with a smaller number of studies examining disease severity or phenotypes.…”

Section: Methodsmentioning

confidence: 99%

“…In the search for biomarkers several possible validation strategies can be used, including: (1) testing on a separately recruited and ascertained validation cohort; (2) using a hold-out data set; and (3) performing permutation testing by label shuffling. 24,25 Eight 5,7,8,14,15,17,19,21 and four 9,12,13,20 studies used permutation and a hold-out dataset, respectively. Both approaches provided support for accuracy of the reported biomarkers.…”

Section: Metabolomic Biomarkers Of Asthmamentioning

confidence: 99%

See 2 more Smart Citations

Asthma Metabolomics and the Potential for Integrative Omics in Research and the Clinic

Kelly

Dahlin

McGeachie

et al. 2017

Chest

149

135

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Asthma is a complex disease well-suited to metabolomic profiling, both for the development of novel biomarkers and for the improved understanding of pathophysiology. In this review, we summarize the 21 existing metabolomic studies of asthma in humans, all of which reported significant findings and concluded that individual metabolites and metabolomic profiles measured in exhaled breath condensate, urine, plasma, and serum could identify people with asthma and asthma phenotypes with high discriminatory ability. There was considerable consistency across the studies in terms of the reported biomarkers, regardless of biospecimen, profiling technology, and population age. In particular, acetate, adenosine, alanine, hippurate, succinate, threonine, and trans-aconitate, and pathways relating to hypoxia response, oxidative stress, immunity, inflammation, lipid metabolism and the tricarboxylic acid cycle were all identified as significant in at least two studies. There were also a number of nonreplicated results; however, the literature is not yet sufficiently developed to determine whether these represent spurious findings or reflect the substantial heterogeneity and limited statistical power in the studies and their methods to date. This review highlights the need for additional asthma metabolomic studies to explore these issues, and, further, the need for standardized methods in the way these studies are conducted. We conclude by discussing the potential of translation of these metabolomic findings into clinically useful biomarkers and the crucial role that integrated omics is likely to play in this endeavor.CHEST 2017; 151(2):262-277

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Metabolomic Biomarkers Of Asthmamentioning

confidence: 99%

See 1 more Smart Citation

Asthma Metabolomics and the Potential for Integrative Omics in Research and the Clinic

Kelly

Dahlin

McGeachie

et al. 2017

Chest

149

135

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“…Ibrahim and colleagues [16] determined and validated discriminatory metabolomic profiles in adult asthmatic patients. In this study, asthma phenotypes based on sputum inflammatory cells, asthma control and inhaled corticosteroid use were investigated.…”

Section: Phenotyping Ebcmentioning

confidence: 99%

Metabolomics of Exhaled Breath Condensate: A Means for Phenotyping Respiratory Diseases?

Maniscalco

Motta

2017

Biomark. Med.

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“…In addition, the simplicity of sample preparation, low volume requirement (typically a few hundred microliters), non-destructive measurement, and last but not least cost efficiency led to rapid acceptance of NMR in noninvasive diagnostics (Motta et al, 2012;Ibrahim et al, 2013;Nagana Gowda and Raftery, 2015;Soininen et al, 2015). The most outstanding point is that hundreds of metabolites can be analyzed in just one NMR measurement (Duarte et al, 2014).…”

Section: Prostate Cancermentioning

confidence: 99%

Nuclear magnetic resonance spectroscopy as a new approach for improvement of early diagnosis and risk stratification of prostate cancer

Yang

Lü

Wen

et al. 2017

J. Zhejiang Univ. Sci. B

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Prostate cancer (PCa) is the second most common male cancer worldwide and the fifth leading cause of death from cancer in men. Early detection and risk stratification is the most effective way to improve the survival of PCa patients. Current PCa biomarkers lack sufficient sensitivity and specificity to cancer. Metabolite biomarkers are evolving as a new diagnostic tool. This review is aimed to evaluate the potential of metabolite biomarkers for early detection, risk assessment, and monitoring of PCa. Of the 154 identified publications, 27 and 38 were original papers on urine and serum metabolomics, respectively. Nuclear magnetic resonance (NMR) is a promising method for measuring concentrations of metabolites in complex samples with good reproducibility, high sensitivity, and simple sample processing. Especially urine-based NMR metabolomics has the potential to be a cost-efficient method for the early detection of PCa, risk stratification, and monitoring treatment efficacy.

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Breath metabolomic profiling by nuclear magnetic resonance spectroscopy in asthma

Cited by 46 publications

References 33 publications

Asthma Metabolomics and the Potential for Integrative Omics in Research and the Clinic

Asthma Metabolomics and the Potential for Integrative Omics in Research and the Clinic

Metabolomics of Exhaled Breath Condensate: A Means for Phenotyping Respiratory Diseases?

Nuclear magnetic resonance spectroscopy as a new approach for improvement of early diagnosis and risk stratification of prostate cancer

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