Breath Analysis: Analytical Methodologies and Clinical Applications

Ceccarini, Alessio; Francesco, Fabio Di; Fuoco, Roger; Ghimenti, Silvia; Onor, Massimo; Tabucchi, S.; Trivella, Maria Giovanna

doi:10.1002/9781118271858.ch23

Cited by 7 publications

(9 citation statements)

References 177 publications

(197 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…shows that patients belonging to different NYHA functional classes are not separated, as the large intersubject variability of clinical and breath data could hide other possible differences, as discussed for acetone from other authors [46]. For these reasons, we postulate that patient's monitoring by breath analysis would be more successful if performed in single subjects over time, as we already reported for breath measurements in general terms [10]. In this way, each patient would act as his/her own control and variations of breath composition could be correlated more easily to the disease evolution [47].…”

Section: Application To Real Samples: Hf Patientsmentioning

confidence: 70%

“…Breath analysis by NTD can be a potential tool to diagnose diseases at an early stage or to monitor their evolution, especially in the case of chronic diseases such as heart failure (HF). The composition of the breath depends on several factors whose relative effect is very difficult to quantify [10,11]. To improve the interpretation of the experimental findings, a more indepth knowledge of the metabolic processes and their relationship with the presence of volatile organic compounds (VOCs) in the breath samples is needed.…”

Section: Introductionmentioning

confidence: 99%

“…Breath analysis is extraordinarily appealing for an effective, easy, painless and non-invasive monitoring of HF patients because the chemical composition of exhaled breath reflects in real time the physiological and pathophysiological processes occurring in the body [10,17,18]. Generally, the analysis of VOCs in exhaled breath is carried out off-line by (i) collecting the sample in sampling bags (e.g.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Determination of volatile organic compounds in exhaled breath of heart failure patients by needle trap micro-extraction coupled with gas chromatography-tandem mass spectrometry

et al. 2017

View full text Add to dashboard Cite

The analytical performances of needle trap micro-extraction (NTME) coupled with gas chromatography-tandem mass spectrometry were evaluated by analyzing a mixture of twenty-two representative breath volatile organic compounds (VOCs) belonging to different chemical classes (i.e. hydrocarbons, ketones, aldehydes, aromatics and sulfurs). NTME is an emerging technique that guarantees detection limits in the pptv range by pre-concentrating low volumes of sample, and it is particularly suitable for breath analysis. For most VOCs, detection limits between 20 and 500 pptv were obtained by pre-concentrating 25 ml of a humidified standard gas mixture at a flow rate of 15 ml min. For all compounds, inter- and intra-day precisions were always below 15%, confirming the reliability of the method. The procedure was successfully applied to the analysis of exhaled breath samples collected from forty heart failure (HF) patients during their stay in the University Hospital of Pisa. The majority of patients (about 80%) showed a significant decrease of breath acetone levels (a factor of 3 or higher) at discharge compared to admission (acute phase) in correspondence to the improved clinical conditions during hospitalization, thus making this compound eligible as a biomarker of HF exacerbation.

show abstract

Section: Application To Real Samples: Hf Patientsmentioning

confidence: 70%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Determination of volatile organic compounds in exhaled breath of heart failure patients by needle trap micro-extraction coupled with gas chromatography-tandem mass spectrometry

et al. 2017

View full text Add to dashboard Cite

show abstract

“…Non-conventional fluids, such as breath and saliva, represent easy, painless and non-invasive ways of mirroring physiological and pathophysiological conditions in the whole body [8]. Volatile organic compounds (VOCs) are produced during biochemical pathways in cells, transported via the bloodstream to the lung and exhaled within minutes after their generation [9]. Saliva contains numerous biological molecules which are directly related to the major physiological systems or are products of their activity [10].…”

Section: A Identification Of /Breath/saliva Biomarkersmentioning

confidence: 99%

HEARTEN: An integrated mHealth platform for holistic HF management

Karanasiou

Tripoliti

Goletsis

et al. 2018

2018 IEEE EMBS International Conference on Biomedical &Amp; Health Informatics (BHI)

Self Cite

View full text Add to dashboard Cite

Heart Failure (HF) is a chronic disease with a continuously increasing incidence and prevalence. HF patients need to cope with often re-admissions and advert events. Patient adherence in medication, nutrition and physical activity guidelines becomes a critical factor that can significantly reduce or even prevent re-hospitalizations and improve quality of life. HEARTEN, an integrated mHealth platform for holistic HF management can significantly contribute to effective and efficient (self-) management of the HF patients, through the integration of novel breath and saliva biosensors, sensors and machine learning and knowledge management techniques. I. INTRODUCTION Heart Failure (HF) is a chronic disease with a continuously increasing incidence and prevalence. This cardiovascular syndrome is expected to increase the next 25 years, double the incidence rates and increase its prevalence 10 fold from age 60 to age 80 [1]. Currently, 15 million people are living with HF in Europe [2]. In the current clinical practice, the management of HF patients involves different experts. The latter examine the patients in a frequent basis and provide a variety of treatment suggestions and recommendations. Current guidelines of the

show abstract

“…Off‐line analyses often involve collecting breath samples into containers prior to the extraction or analysis of the VOCs . The breath sampling procedure is a critical step, as is the preservation of the sample integrity during storage, especially so into polymer bags . Surface adsorption of analytes, desorption of impurities and permeation/diffusion through the bag material in both directions can affect the composition of the sample .…”

Section: Introductionmentioning

confidence: 99%

Volatile compounds released by Nalophan; implications for selected ion flow tube mass spectrometry and other chemical ionisation mass spectrometry analytical methods

Michalčíková

Dryahina

Smith

et al. 2020

Rapid Comm Mass Spectrometry

View full text Add to dashboard Cite

Rationale Nalophan bags are commonly used to collect breath samples for volatile metabolite analysis. Volatile organic compounds (VOCs) released from the polymer can, however, be mistaken as breath metabolites when analyses are performed by selected ion flow tube mass spectrometry, SIFT‐MS, or techniques that depend on a proper understanding of ion chemistry. Methods Three analytical techniques were used to analyse the VOCs released into the nitrogen used to expand Nalophan bags, viz. gas chromatography/mass spectrometry (GC/MS), secondary electrospray ionization mass spectrometry (SESI‐MS) and selected ion flow tube mass spectrometry (SIFT‐MS). The most significant VOCs were identified and quantified by SIFT‐MS as a function of storage time, temperature and humidity. Results The consistent results obtained by these three analytical methods identify 1,2‐ethanediol (ethylene glycol) and 2‐methyl‐1,3‐dioxolane as the major VOCs released by the Nalophan. Their concentrations are enhanced by increasing the bag storage temperature and time, reaching 170 parts‐per‐billion by volume (ppbv) for ethylene glycol and 34 ppbv for 2‐methyl‐1,3‐dioxolane in humid nitrogen (absolute humidity of 5%) contained in an 8‐L Nalophan bag stored at 37°C for 160 min. Conclusions Using H3O+ reagent ions for SIFT‐MS and SESI‐MS analyses, the following analyte ions (m/z values) are affected by the Nalophan impurities: 45, 63, 81, 89 and 99, which can compromise analyses of acetaldehyde, ethylene glycol, monoterpenes, acetoin, butyric acid, hexanal and heptane.

show abstract

Breath Analysis: Analytical Methodologies and Clinical Applications

Cited by 7 publications

References 177 publications

Determination of volatile organic compounds in exhaled breath of heart failure patients by needle trap micro-extraction coupled with gas chromatography-tandem mass spectrometry

Determination of volatile organic compounds in exhaled breath of heart failure patients by needle trap micro-extraction coupled with gas chromatography-tandem mass spectrometry

HEARTEN: An integrated mHealth platform for holistic HF management

Volatile compounds released by Nalophan; implications for selected ion flow tube mass spectrometry and other chemical ionisation mass spectrometry analytical methods

Contact Info

Product

Resources

About