Breast Tumor Cell Lines From Pleural Effusions2

Abstract

Summary During 1973, 4 new epithelial tumor cell lines were isolated from pleural effusions from breast cancer patients. We describe 3 of these lines: MDA-MB-134, with a mean chromosome number of 43; MDA-MB-175, with a mean chromosome number of 49; and MDA-MB-231, with a mean chromosome number between 65 and 69. We isolated the same cell type from 4 of 10 effusions from MDA-MB-134 and from 6 of 8 effusions from MDA-MB-175. We found that pleural effusions as a source of breast tumor cells to be cultured and studied in vitro have the following advantages: 1) large amounts of material and the possibility of obtaining sequential samples from the same patient; 2) high viability of tumor cells; 3) scarcity or absence of fibroblasts; and 4) the possibility of separating the tumor cells from other “contaminating” cell types by differences in their speed or degree of attachment to the flask. All lines from different patients differed, as seen grossly and microscopically. All lines from sequential pleural effusions from the same patient were apparently alike. No viruses or mycoplasmas were detected in any line.

Comprehensive genetic and functional characterization of IPH‐926: a novel CDH1‐null tumour cell line from human lobular breast cancer

“…Both cell lines are characterized by grape-like clusters of small, round cells and the occasional appearance of larger, flattened cells ( Figure 1A) [21,22]. In comparison to IPH-926 cells (doubling time of about 2 weeks), MDA-MB-134 cells proliferate more rapidly (reported doubling times of 22-60 h) [18,22,43].…”

“…Recently, it was proposed that the cell line MDA-MB-134 might be derived from an ILBC [10]. However, MDA-MB-134 was originally reported to be derived from a ductal mammary carcinoma [21] is widely perceived as a ductal breast cancer cell line [e.g., 15], usually non-tumourigenic in vivo [15,18,[21][22][23] and lacking E-cadherin expression due to a homozygous mutation of the CDH1 gene [24]. However, identical allozyme phenotype patterns in the MDA-MB-134 and the MDA-MB-309 cell lines raised concerns about a potential cross-contamination or mis-identification of both cell lines early after their establishment [25].…”

Proteasome inhibitor induced gene expression profiles reveal overexpression of transcriptional regulators ATF3, GADD153 and MAD1

“…The human breast cancer cell line MCF7 was obtained from American Tissue Culture Collections (ATCC number HTB-22) (Giard et al, 1973;Cailleau et al, 1974). MCF7 cells were cultured at 378C in a 5.5% CO 2 atmosphere in Minimum Essential Medium alpha with ribonucleosides and deoxyribonucleosides, supplemented with 10% fetal calf serum, 2 mM L-glutamine, 1 mM sodium pyruvate, 0.1 mM non-essential amino acids and 0.01 mg/ml gentamycin (Gibco ± BRL, Life Technologies AG, Basel, Switerland).…”

SR31747A is a sigma receptor ligand exhibiting antitumoural activity both in vitro and in vivo

“…This variant cell line, selected in vitro for its resistance to the growth-inhibitory effects of the antioestrogen LY117018 (Davidson et al, 1986), was maintained in DMEM/F12 (1 : 1, v v À1 ) supplemented with 10% FBS. The hormono-unresponsive MDA-MB-321 (Cailleau et al, 1974) and BT-20 (Lasfargues and Ozzello, 1968) cell lines were also established from a metastatic human breast cancer tumour. MDA-MB-231 cells were maintained in Leibovitz L15 culture medium supplemented with 10 mM Hepes buffer, 6 mg ml À1 human insulin, 2 mM L-glutamine, 1% nonessential amino acids, 50 UI ml À1 penicillin, 50 mg ml À1 streptomycin and 10% FBS.…”