Breast tissue ablation with irreversible electroporation in rabbits: A safety and feasibility study

Zhang, Wenlong; Wang, Wanning; Chai, Wei; Luo, Xiaomei; Li, Jiannan; Shi, Jian; Bi, Liqi; Niu, Lizhi

doi:10.1371/journal.pone.0181555

Cited by 10 publications

(7 citation statements)

References 45 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…After the IRE ablation, the pigs were closely monitored by experienced veterinarians every 8 hr for 24 hr, and no critical complications were observed. Subsequent necropsy showed no evidence of perforation, peritonitis, or injury of vascular structures, similar to other studies conducted in different organs [ 21 - 23 ]. Apoptosis, the result of electroporation, was confirmed by histopathologic evaluation; however, the evaluation showed insufficient depth of the ablated ductal wall.…”

Section: Discussionsupporting

confidence: 85%

“…Subsequent necropsy showed no evidence of perforation, peritonitis, or injury of vascular structures, similar to other studies conducted in different organs. [21][22][23] Apoptosis, the result of electroporation, was confirmed by histopathologic evaluation; however, the evaluation showed insufficient depth of the ablated ductal wall. Compared with other studies, our study used lower energy levels as the wall of the bile duct with a tunnel-type structure is thinner than that of other solid organs such as the liver and pancreas.…”

Section: Discussionmentioning

confidence: 89%

See 1 more Smart Citation

Novel Ablation Therapy Using Endoscopic Irreversible Electroporation in the Bile Duct: A Pilot Animal Study

Lee

Choi

et al. 2021

Clin Endosc

View full text Add to dashboard Cite

Background/Aims Irreversible electroporation (IRE) is a relatively new ablation method. However, the application of IRE ablation in the treatment of biliary disease has not been attempted. A minimally invasive approach using endoscopic retrograde cholangiopancreatography (ERCP) can be a novel therapeutic modality for IRE ablation. In this study, we aimed to investigate the feasibility of endoscopic IRE for the biliary tract using an animal model. Methods A new catheter-type electrode was developed for endoscopic IRE ablation of the biliary tract. We performed ERCP and endoscopic IRE ablations in the normal common bile duct of Yorkshire pigs. The experimental setting of IRE was 500 V/cm (50 pulses, 100-µs length). The animals were sacrificed after 24 hr, and the ablated bile duct was examined. Results Well-demarcated focal color changes were observed on the mucosa of the common bile duct. The depth of change after IRE was confined to the mucosal and submucosal layers. Apoptotic changes in the bile duct were observed only around the IRE ablation area. Immunohistochemistry assay showed cell death in the bile duct along the electrode. Conclusions Endoscopic IRE ablation using ERCP was successfully performed in the common bile duct. It can be a potential option for the treatment of biliary tumors.

show abstract

Section: Discussionsupporting

confidence: 85%

Section: Discussionmentioning

confidence: 89%

Novel Ablation Therapy Using Endoscopic Irreversible Electroporation in the Bile Duct: A Pilot Animal Study

Lee

Choi

et al. 2021

Clin Endosc

View full text Add to dashboard Cite

show abstract

“…Transmission electron microscopy (TEM) studies of electroporated tissue are very scarce, and virtually non-existent scanning electron microscope (SEM) studies 9 . To our knowledge, there are two studies that describe TEM findings in uterine cervix and breast in rabbits 10,11 and a study in pig liver which uses TEM methodology after IRE using needle but not parallel-plate electrodes 12 .…”

Section: Introductionmentioning

confidence: 99%

Histopathological and Ultrastructural Changes after Electroporation in Pig Liver Using Parallel-Plate Electrodes and High-Performance Generator

López-Alonso

Hernaez

Sarnago

et al. 2019

Sci Rep

View full text Add to dashboard Cite

Irreversible electroporation (IRE) has gained attention as a new non-thermal therapy for ablation with important benefits in terms of homogeneous treatment and fast recovery. In this study, a new concept of high voltage generator is used, enabling irreversible electroporation treatment in large tissue volume using parallel plates. Unlike currently available generators, the proposed versatile structure enables delivering high-voltage high-current pulses. To obtain homogeneous results, 3-cm parallel-plates electrodes have also been designed and implemented. IRE ablation was performed on six female pigs at 2000 V/cm electric field, and the results were analysed after sacrifice three hours, three days and seven days after ablation. Histopathological and ultrastructural studies, including transmission and scanning electron microscopy, were carried out. The developed high-voltage generator has proved to be effective for homogeneous IRE treatment using parallel plates. The destruction of the membrane of the hepatocytes and the alterations of the membranes of the cellular organelles seem incompatible with cell death by apoptosis. Although endothelial cells also die with electroporation, the maintenance of vascular scaffold allows repairing processes to begin from the third day after IRE as long as the blood flow has not been interrupted. This study has opened new direction for IRE using high performance generators and highlighted the importance of taking into account ultrastructural changes after IRE by using electron microscopy analysis.

show abstract

“… 31 On the other hand, in vivo studies have been reported in animal models comprising nude mice 32 and rabbits. 33 The results reported in these works suggest that IRE may be a promising approach for patients with breast cancer who are not eligible for surgical excision. In addition, numerical modeling of electric field distribution represents a useful tool to validate the required voltage to fully expose the target to the electric field determined from in vitro experiments.…”

Section: Introductionmentioning

confidence: 91%

“…Nonetheless, more experimental evidence regarding the treatment of breast cancer with IRE is required, since reports on this field remain limited. 31 - 34 …”

Section: Introductionmentioning

confidence: 99%

Computational Feasibility Analysis of Electrochemotherapy With Novel Needle-Electrode Arrays for the Treatment of Invasive Breast Ductal Carcinoma

Vera-Tizatl

Vera-Hernandez

et al. 2018

Technol Cancer Res Treat

View full text Add to dashboard Cite

Breast cancer represents a rising problem concerning public health worldwide. Current efforts are aimed to the development of new minimally invasive and conservative treatment procedures for this disease. A treatment approach for invasive breast ductal carcinoma could be based on electroporation. Hence, in order to determine the effectiveness of electrochemotherapy in the treatment of this disease, 12 electrode models were investigated on realistic patient-specific computational breast models of 3 patients diagnosed by Digital Breast Tomosynthesis imaging. The electrode models exhibit 4, 5, and 6 needles arranged in 4 geometric configurations (delta, diamond, and star) and 3 different needle spacing resulting in a total of 12 needle-electrode arrays. Electric field distribution in the tumors and a surrounding safety margin of 1 cm around the tumor edge is computed using the finite element method. Efficiency of the electrode arrays was determined hierarchically based on (1) percentage of tumor volume reversibly electroporated, (2) percentage of tumor volume irreversibly electroporated, (3) percentage of treated safety margin volume, (4) minimal invasiveness, that is, minimal number of electrodes used, (5) minimal activated electrode pairs, and (6) minimal electric current. Results show that 3 electrode arrays (4 needle-delta, 5 needle-diamond, and 6 needle-star) with fixed-geometry configuration could be used in the treatment with electrochemotherapy of invasive breast ductal carcinomas ranging from 1 to 5 cm3 along with a surrounding safety margin of 1 cm.

show abstract

Breast tissue ablation with irreversible electroporation in rabbits: A safety and feasibility study

Cited by 10 publications

References 45 publications

Novel Ablation Therapy Using Endoscopic Irreversible Electroporation in the Bile Duct: A Pilot Animal Study

Novel Ablation Therapy Using Endoscopic Irreversible Electroporation in the Bile Duct: A Pilot Animal Study

Histopathological and Ultrastructural Changes after Electroporation in Pig Liver Using Parallel-Plate Electrodes and High-Performance Generator

Computational Feasibility Analysis of Electrochemotherapy With Novel Needle-Electrode Arrays for the Treatment of Invasive Breast Ductal Carcinoma

Contact Info

Product

Resources

About