Breast Milk Anti‐<i>Escherichia coli</i> Heat‐Labile Toxin IgA Antibodies Protect against Toxin‐Induced Infantile Diarrhea

Cruz, J. R.; Gil, Liliana Álvarez; Cano, Floridalma; Cáceres, Patricia; Pareja, Gilda

doi:10.1111/j.1651-2227.1988.tb10726.x

Cited by 104 publications

(45 citation statements)

References 20 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The protection against diarrhogenic bacteria and their toxins appears to be mediated via the milk SIgA antibodies, according to studies of infections with enterotoxigenic E. coli, Shigella, V. cholerae and G. lamblia (Glass et al 1983;Cruz et al 1988;Ruiz-Palacios et al 1990;Hayani et al 1992;Long et al 1999). The human peptide anti-secretory factor, which can be induced in human milk (Svensson et al 2004), has recently been shown to give protection against acute diarrhoea as well as prolonged diarrhoea (Zaman et al 2007).…”

Section: Breast-feeding and Diarrhoeamentioning

confidence: 99%

“…In clinical studies of breast-feeding it has been demonstrated that the levels of SIgA antibodies in the milk relate to protection against infections caused by enterotoxigenic E. coli, Vibrio cholerae, Campylobacter, Shigella and Giardia lamblia (Glass et al 1983;Cruz et al 1988;RuizPalacios et al 1990;Hayani et al 1992;Walterspiel et al 1994;Long et al 1999).…”

Section: Secretory Igamentioning

confidence: 99%

See 1 more Smart Citation

Session 1: Feeding and infant development Breast-feeding and immune function

2007

View full text Add to dashboard Cite

The newborn receives, via the placenta, maternal IgG antibodies against the microbes present in its surroundings, but such antibodies have a pro-inflammatory action, initiating the complement system and phagocytes. Although the host defence mechanisms of the neonate that involve inflammatory reactivity are somewhat inefficient, this defence system can still have catabolic effects. Breast-feeding compensates for this relative inefficiency of host defence in the neonate by providing considerable amounts of secretory IgA antibodies directed particularly against the microbial flora of the mother and her environment. These antibodies bind the microbes that are appearing on the infant's mucosal membranes, preventing activation of the pro-inflammatory defence. The major milk protein lactoferrin can destroy microbes and reduce inflammatory responses. The non-absorbed milk oligosaccharides block attachment of microbes to the infant's mucosae, preventing infections. The milk may contain anti-secretory factor, which is anti-inflammatory, preventing mastitis in mothers and diarrhoea in infants. Numerous additional factors in the milk are of unknown function, although IL-7 is linked to the larger size of the thymus and the enhanced development of intestinal Tgd lymphocytes in breast-fed compared with non-breast-fed infants. Several additional components in the milk may help to explain why breast-feeding can reduce infant mortality, protecting against neonatal septicaemia and meningitis. It is therefore important to start breast-feeding immediately. Protection is also apparent against diarrhoea, respiratory infections and otitis media. There may be protection against urinary tract infections and necrotizing enterocolitis, and possibly also against allergy and certain other immunological diseases, and tumours. In conclusion, breast-feeding provides a very broad multifactorial anti-inflammatory defence for the infant.

show abstract

Section: Breast-feeding and Diarrhoeamentioning

confidence: 99%

Section: Secretory Igamentioning

confidence: 99%

Session 1: Feeding and infant development Breast-feeding and immune function

2007

View full text Add to dashboard Cite

show abstract

“…Studies evaluating colostral leukocytes have shown them to mediate ADCC in vitro (24), but inasmuch as ADCC antibody was not present in this study, their role in vivo in this situation is questionable. Five studies have identified secretory IgA in human milk as the protective agent in the prevention of illness (26)(27)(28)(29)(30). However, the possible role of anti-HSV IgA milk antibodies as detailed by WBA in protection against HSV infection is currently unknown.…”

Section: Discussionmentioning

confidence: 99%

“…Studies in humans have linked protection against cholera (26), Escherichia coli toxin-mediated enteritis (27), Campylobacter diarrhea (28), and neonatal rotavirus infection (29. 30) with secretory IgA in milk.…”

mentioning

confidence: 99%

A Comparison of Herpes Simplex Virus Specific Antibodies Found in Human Milk and Serum

et al. 1992

View full text Add to dashboard Cite

ABSTRACT. It is not known if milk antibody protects infants from herpes simplex virus (HSV) infection. As a first step to test this hypothesis, anti-HSV antibodies were studied in human milk. Paired serum and milk samples were analyzed for anti-HSV antibodies by ELISA, Western blot analysis (WBA), neutralization (NT) plaque assay, and antibody-dependent cellular cytotoxicity (ADCC) assay. Nineteen of the 20 serum samples showed anti-HSV activity by ELISA and ADCC, and 18 showed activity by WBA and NT. We found a significant association between the immunoassays for detection of anti-HSV antibodies in sera. Fewer of the human milk samples showed anti-HSV activity; only one milk sample was positive by ELISA and one by N T assay, four by ADCC and 12 by WBA. The milk sample from the seronegative donor was also negative. We found a poor association of antibody titers in human milk and serum antibody titers using ELISA, NT, and ADCC assays. There was a significant (p = 0.022) association between serum and milk results using WBA. Among the four assays, WBA was the most sensitive for antibody detection. It will be used in an on-going prospective study to determine the role of anti-HSV antibody in the protection against HSV infections in infants. (Pediatr Res 31: 591-595,1992)

show abstract

“…: +55 (61) 3307-2557; Fax: +55 (61) 3273-4608. E-mail: giuglian@unb.br virulence factors (7,8). Recently, some studies of our group demonstrated that sIgA inhibits the adherence of diffusely adherent Escherichia coli (DAEC), enteroaggregative Escherichia coli (EAEC) and Shigella flexneri to HeLa cells (1,31).…”

Section: Introductionmentioning

confidence: 99%

Inhibition of enterotoxigenic Escherichia coli (ETEC) adhesion to Caco-2 cells by human milk and its immunoglobulin and non-immunoglobulin fractions

Oliveira¹,

Bessler²,

Báo³

et al. 2007

Braz. J. Microbiol.

View full text Add to dashboard Cite

Enterotoxigenic Escherichia coli (ETEC) is the most common cause of diarrhea in children in developing countries and among travelers to ETEC endemic areas. ETEC diarrhea is caused by colonization of the small intestine mediated by colonization factor (CF) antigens, and subsequent elaboration of enterotoxins. Breast feeding has been related to protection against enteric infections. The protective effect of human milk can be ascribed to its immunoglobulin content, specially secretory immunoglobulin A (sIgA), and to nonimmunoglobulin components such as free oligosaccharides, glycoproteins and glycolipids. In this study we investigated the effect of whole human milk and its fractions immunoglobulin and non-immunoglobulin on the adherence of ETEC strains possessing different CFs to Caco-2 cells, as well as the ability of sIgA and free secretory component (fSC) to bind to bacterial superficial proteins. Pooled human milk from three donors were fractionated by gel filtration and analyzed by SDS-PAGE. Our results revealed that whole human milk and its proteins fractions, containing sIgA and fSC, inhibited adhesion ETEC strains harboring different colonization factors antigens. We also verified that sIgA and fSC, using immunoblotting and immunogold labeling assays, bound to some fimbrial proteins and other material present in bacterial surface. Our findings suggest that whole human milk and its fractions may contribute to protection against ETEC infections by blocking bacterial adhesion mediated by different colonization antigens.

show abstract

Breast Milk Anti‐Escherichia coli Heat‐Labile Toxin IgA Antibodies Protect against Toxin‐Induced Infantile Diarrhea

Cited by 104 publications

References 20 publications

Session 1: Feeding and infant development Breast-feeding and immune function

Session 1: Feeding and infant development Breast-feeding and immune function

A Comparison of Herpes Simplex Virus Specific Antibodies Found in Human Milk and Serum

Inhibition of enterotoxigenic Escherichia coli (ETEC) adhesion to Caco-2 cells by human milk and its immunoglobulin and non-immunoglobulin fractions

Contact Info

Product

Resources

About