Breast development gives insights into breast disease

Osin, Peter; Anbazhagan, Ramaswamy; Bártková, Jiřina; Nathan, B.; Gusterson, Barry A.

doi:10.1046/j.1365-2559.1998.00479.x

Cited by 40 publications

(24 citation statements)

References 46 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…In support of this idea, TN-C is expressed around the neck of the breast bud, and surrounding portions of the branching ducts during mammary gland development. 57 Our findings are also consistent with recent reports that showed that overexpression of c-met in MCF-10A cells and in the mouse mammary gland leads to increased branching and intraluminal filling, respectively. 12,58 Furthermore, a link between TN-C and cmet has been previously demonstrated in colon cancerassociated myofibroblasts, which secrete both TN-C and HGF, and have been shown to promote colon cancer cell invasion.…”

Section: Tenascin-c Controls C-met Function In 3d 835supporting

confidence: 93%

Quantitative Analysis of Three-Dimensional Human Mammary Epithelial Tissue Architecture Reveals a Role for Tenascin-C in Regulating c-Met Function

Taraseviciute

Vincent

Schedin

et al. 2010

The American Journal of Pathology

View full text Add to dashboard Cite

Remodeling of the stromal extracellular matrix and elevated expression of specific proto-oncogenes within the adjacent epithelium represent cardinal features of breast cancer, yet how these events become integrated is not fully understood. To address this question, we focused on tenascin-C (TN-C), a stromal extracellular matrix glycoprotein whose expression increases with disease severity. Initially, nonmalignant human mammary epithelial cells (MCF-10A) were cultured within a reconstituted basement membrane (BM) where they formed three-dimensional (3-D) polarized, growth-attenuated, multicellular acini, enveloped by a continuous endogenous BM. In the presence of TN-C, however, acini failed to generate a normal BM, and net epithelial cell proliferation increased. To quantify how TN-C alters 3-D tissue architecture and function, we developed a computational image analysis algorithm, which showed that although TN-C disrupted acinar surface structure, it had no effect on their volume. Thus, TN-C promoted epithelial cell proliferation leading to luminal filling, a process that we hypothesized involved c-met, a proto-oncogene amplified in breast tumors that promotes intraluminal filling. Indeed, TN-C increased epithelial c-met expression and promoted luminal filling, whereas blockade of c-met function reversed this phenotype, resulting in normal BM deposition, proper lumen formation, and decreased cell proliferation. Collectively, these studies, combining a novel quantitative image analysis tool with 3-D organotypic cultures, demonstrate that stromal changes associated with breast cancer can control proto-oncogene function.

show abstract

Section: Tenascin-c Controls C-met Function In 3d 835supporting

confidence: 93%

Quantitative Analysis of Three-Dimensional Human Mammary Epithelial Tissue Architecture Reveals a Role for Tenascin-C in Regulating c-Met Function

Taraseviciute

Vincent

Schedin

et al. 2010

The American Journal of Pathology

View full text Add to dashboard Cite

show abstract

“…Most of our knowledge of mammary gland development has been derived from observations made in mice and interpolated for humans despite the well-known differences between human and mouse mammary gland development and function. Studies addressing human mammary gland development have been limited to the structural and immunohistochemical analyses of a limited number of samples collected at different stages of fetal, infantile, childhood, and pubertal development Osin et al 1998;Naccarato et al 2000;Jolicoeur et al 2003).…”

Section: The Role Of the Microenvironment In Mammary Gland Developmenmentioning

confidence: 99%

The Role of the Microenvironment in Mammary Gland Development and Cancer

Polyák

Kalluri

2010

Cold Spring Harbor Perspectives in Biology

240

187

View full text Add to dashboard Cite

The mammary gland is composed of a diverse array of cell types that form intricate interaction networks essential for its normal development and physiologic function. Abnormalities in these interactions play an important role throughout different stages of tumorigenesis. Branching ducts and alveoli are lined by an inner layer of secretory luminal epithelial cells that produce milk during lactation and are surrounded by contractile myoepithelial cells and basement membrane. The surrounding stroma comprised of extracellular matrix and various cell types including fibroblasts, endothelial cells, and infiltrating leukocytes not only provides a scaffold for the organ, but also regulates mammary epithelial cell function via paracrine, physical, and hormonal interactions. With rare exceptions breast tumors initiate in the epithelial compartment and in their initial phases are confined to the ducts but this barrier brakes down with invasive progression because of a combination of signals emitted by tumor epithelial and various stromal cells. In this article, we overview the importance of cellular interactions and microenvironmental signals in mammary gland development and cancer.

show abstract

“…EGF is essential for mammary ductal growth and branching {Kamalati, 1999 #374}. Both EGF and HGF work with transforming growth factor alpha (TGF a ), another mitogen [159] , to promote lobuloalveolar development [160] .…”

Section: Other Regulators Of Breast Developmentmentioning

confidence: 99%

“…As secondary outgrowths vertically penetrate the mesenchyme [33] , each projection has a slender stalk with a bulbous end and is covered by a continuous BM [159] . The papillary layer of the dermis encases the growing cords and gives rise to the vascularized fi brous tissue around ducts and within the lobules.…”

Section: Events Of Prenatal Breast Developmentmentioning

confidence: 99%

“…BRCA1 , a tumor suppressor gene is expressed at a high level in human fetal breasts between week 21 and 26 of gestation and is closely associated with differentiation [185] . TGF-a is expressed in the developing breast where it promotes both proliferation and differentiation [159] . It is localized to the developing stroma and the epithelial bud.…”

Section: Genes Transcription Factors and Growth Factors During Prenamentioning

confidence: 99%

See 1 more Smart Citation

Anatomy and Physiology of the Breast

Johnson

2010

Management of Breast Diseases

View full text Add to dashboard Cite

Breast development gives insights into breast disease

Abstract: The distribution of the growth factors and extracellular matrix proteins within the developing human breast indicates that they play a significant role in different cellular compartments during morphogenesis and provides insights into breast disease.

Cited by 40 publications

References 46 publications

Quantitative Analysis of Three-Dimensional Human Mammary Epithelial Tissue Architecture Reveals a Role for Tenascin-C in Regulating c-Met Function

Quantitative Analysis of Three-Dimensional Human Mammary Epithelial Tissue Architecture Reveals a Role for Tenascin-C in Regulating c-Met Function

The Role of the Microenvironment in Mammary Gland Development and Cancer

Anatomy and Physiology of the Breast

Contact Info

Product

Resources

About