Breast Cancer Biology: The Multifaceted Roles of Mesenchymal Stem Cells

Patel, Shyam A.; Heinrich, Andrew C.; Reddy, Bobby Y.; Srinivas, Balaji Kyathegowdanadoddi; Heidaran, Nicole; Rameshwar, Pranela

doi:10.1155/2008/425895

Cited by 15 publications

(13 citation statements)

References 42 publications

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“…VEGF and IL‐8 regulate not only cancer initiation but also progression. TGF‐β has a bimodal action on cancer, it alters the microenvironment and immune responses to provide favorable conditions for cancer maintenance; however it also acts as a tumor suppressor 64. TGF‐β2 is a potent regulator of osteoclastic bone resorption,65 production of matrix metalloproteinase‐9 and epithelial‐to‐mesenchymal transition 66.…”

Section: Resultsmentioning

confidence: 99%

Engineered 3D Silk‐Based Metastasis Models: Interactions Between Human Breast Adenocarcinoma, Mesenchymal Stem Cells and Osteoblast‐Like Cells

Talukdar

Kundu

2013

Adv Funct Materials

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Bone metastasis occurs in 70% of breast cancer patients and is a frequent cause of morbidity in cancer patients. A delicate balance exists in the bone microenvironment, but the functional dynamics underlying the tumor cell‐microenvironment interactions remain poorly understood. 3D in vitro model systems of metastasis can throw new light on this phenomenon. Silk protein fibroin scaffolds, are cytocompatible for 3D cancer cell culture. They are structurally more resistant to protease degradation than other native biomaterials making these matrices suitable for cancer modeling. In this report, human breast adenocarcinoma cells, human osteoblast like cells and mesenchymal stem cells are co‐cultered. Cancer cells and osteoblast‐like cells are found to interact through secreted products. Decreased population of osteoblast‐like cells and mineralization of extracellular matrix are observed as a result of co‐culture. Significantly increased migration of breast cancer cells is observed in the bone‐like constructs than in non‐seeded scaffolds. The co‐culture constructs show significant increase in drug resistance, invasiveness and angiogenicity. Co‐culture of breast cancer cells with osteoblast like cells and mesenchymal stem cells also indicate that the interaction of cancer cells with bone microenvironment varies with spatial organization, presence of osteogenic factors as well as stromal cell type. Here, results show that 3D in vitro co‐culture models is possibly a better system to study and target cancer progression.

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Section: Resultsmentioning

confidence: 99%

Engineered 3D Silk‐Based Metastasis Models: Interactions Between Human Breast Adenocarcinoma, Mesenchymal Stem Cells and Osteoblast‐Like Cells

Talukdar

Kundu

2013

Adv Funct Materials

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“…Whole cell extracts were prepared and analyzed by Western blot, as described (32). Nuclear extracts were prepared with NE-PER nuclear and cytoplasmic extraction reagent (Thermo Scientific).…”

Section: Western Analysesmentioning

confidence: 99%

Gap Junction–Mediated Import of MicroRNA from Bone Marrow Stromal Cells Can Elicit Cell Cycle Quiescence in Breast Cancer Cells

et al. 2011

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Bone marrow (BM) metastasis of breast cancer (BC) can recur even decades after initial diagnosis and treatment, implying the long-term survival of disseminated cancer cells in a dormant state. Here we investigated the role of microRNAs (miRNA) transmitted from BM stroma to BC cells via gap junctions and exosomes in tumor cell quiescence. MDA-MB-231 and T47D BC cells arrest in G 0 phase of the cell cycle when cocultured with BM stroma. Analyses of miRNA expression profiles identified numerous miRNAs implicated in cell proliferation including miR-127, -197, -222, and -223 targeting CXCL12. Subsequently, we showed that these CXCL12-specific miRNAs are transported from BM stroma to BC cells via gap junctions, leading to reduced CXCL12 levels and decreased proliferation. Stroma-derived exosomes containing miRNAs also contributed to BC cell quiescence, although to a lesser degree than miRNAs transmitted via gap junctions. This study shows that the transfer of miRNAs from BM stroma to BC cells might play a role in the dormancy of BM metastases.

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“…MiRNAs have also been proposed to serve as the link between cancer and chronic inflammation, although the precise role of miRNAs in these biological processes remains unclear. 80 Histone modifications. The post-translational acetylation and methylation of lysine residues in histone tails is another epigenetic process that can regulate gene expression.…”

Section: Epigenetic Control Of Tumor Developmentmentioning

confidence: 99%