Breakpoint Junctions of Chromosome 9p Deletions in Two Human Glioma Cell Lines

Abstract

Interstitial deletions of the short arm of chromosome 9 are associated with glioma, acute lymphoblastic leukemia, melanoma, mesothelioma, lung cancer, and bladder cancer. The distal breakpoints of the deletions (in relation to the centromere) in 14 glioma and leukemia cell lines have been mapped within the 400 kb IFN gene cluster located at band 9p21. To obtain information about the mechanism of these deletions, we have isolated and analyzed the nucleotide sequences at the breakpoint junctions in two glioma-derived cell lines. The A1235 cell line has a complex rearrangement of chromosome 9, including a deletion and an inversion that results in two breakpoint junctions. Both breakpoints of the distal inversion junction occurred within AT-rich regions. In the A172 cell line, a tandem heptamer repeat was found on either side of the deletion breakpoint junction. The distal breakpoint occurred 5' of IFNA2; the 256 bp sequenced from the proximal side of the breakpoint revealed 95% homology to long interspersed nuclear elements. One- and two-base-pair overlaps were observed at these junctions. The possible role of sequence overlaps, and repetitive sequences, in the rearrangement is discussed.

Calotropis procera: A double edged sword against glioblastoma, inhibiting glioblastoma cell line growth by targeting histone deacetylases (HDAC) and angiogenesis

“… U87 MG American type culture collection (ATCC cat # HTB-14), U-251 MG (formerly known as U-373 MG cat # 09063001, European Collection of Authenticated Cell Cultures (ECACC)), and A1235 glioblastoma cell line originated from Aaronson S.A.; Mt. Sinai Medical Center; New York; USA [ 22 ]. …”

“…A1235 glioblastoma cell line originated from Aaronson S.A.; Mt. Sinai Medical Center; New York; USA [ 22 ].…”