Breaking physical dormancy of Cassia leptophylla and Senna macranthera (Fabaceae: Caesalpinioideae) seeds: water absorption and alternating temperatures

Paula, Alexandre Souza de; Delgado, Carolina Maria Luzia; Paulilo, Maria Terezinha Silveira; Santos, Marisa

doi:10.1017/s096025851200013x

Cited by 45 publications

(32 citation statements)

References 30 publications

(39 reference statements)

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“…La impermeabilidad de la cubierta se debe a la presencia de una o más capas de células en empalizadas (células de Malpighi) impregnadas con sustancias hidrofóbicas (Rolston, 1978;Baskin & Baskin, 2004;Venier et al, 2012). Se ha documentado que el ingreso del agua ocurre en una región anatómica especializada (como el lente en Fabaceae), que en condiciones favorables, actuaría como una válvula higroscópica permitiendo la imbibición de las semillas (De Paula et al, 2012;Baskin & Baskin, 2014). En condiciones naturales, se ha sugerido que la exposición de las semillas a altas temperaturas o a temperaturas fluctuantes serían los responsables de la salida de dormición (Vazquez-Yañes & Orozco-Segovia, 1982), mientras que en condiciones de laboratorio la escarificación mecánica, térmica y química, así como el almacenamiento en seco, las bajas temperaturas y las altas presiones atmosféricas, facilitarían la salida de dormición de las semillas (ver Baskin & Baskin, 2014).…”

Section: Introductionunclassified

Dormición física y conservación ex situ de semillas de Amburana cearensis y Myroxylon peruiferum (Fabaceae)

Galíndez

Malagrina²,

Ceccato³

et al. 2015

Bol. Soc. Argent. Bot.

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Amburana cearensis y Myroxylon peruiferum son dos especies nativas del noroeste argentino que se encuentran amenazadas por la explotación de su madera, siendo prioritario establecer programas de manejo, restauración ecológica y conservación. La presencia de dormición física en semillas dificulta su utilización en dichos programas. Los objetivos de este trabajo fueron determinar la presencia de dormición física, los métodos de salida de dormición y el comportamiento durante el almacenamiento en semillas de ambas especies. Las semillas de A. cearensis presentaron dormición física y la escarificación mecánica y química fueron los mejores métodos para la salida de dormición de las semillas. Las semillas de M. peruiferum no presentaron dormición física. Las semillas de A. cearensis produjeron semillas con comportamiento ortodoxo, por lo que pueden ser conservadas en bancos de germoplasma, mientras que las semillas de M. peruiferum fueron sensibles a la desecación y al almacenamiento a -18ºC. Estos resultados serán de utilidad para el uso de estas especies en programas de producción de plantas en invernáculo, reforestación, restauración y conservación ex situ en bancos de germoplasma.

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Section: Introductionunclassified

Dormición física y conservación ex situ de semillas de Amburana cearensis y Myroxylon peruiferum (Fabaceae)

Galíndez

Malagrina²,

Ceccato³

et al. 2015

Bol. Soc. Argent. Bot.

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“…However, they observed that depending on the time exposed to sulphuric acid, or in the field, the lens and extra-hilar region also become permeable to water. Another structure that has been mentioned as being important to the entrance of water in seeds of Cassia (Fabaceae), which are physically dormant, is the micropyle (Bhattacharya, & Saha, 1990;Paula, Delgado, Paulilo, & Santos, 2012). For S. tomentosa and E. speciosa in the present work, it was interesting to notice that when only the micropyle was blocked, in scarified seeds, the water uptake was inhibited until the second and fourth day (respectively) of seed incubation.…”

Section: Discussionmentioning

confidence: 50%

Water absorption and dormancy-breaking requirements of physically dormant seeds of Sophora tomentosa L. and Erythrina speciosa Andr. (Fabaceae-Faboideae)

Delgado

Paula²,

Santos³

et al. 2014

RBT

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Dormancy-breaking requirements of Sophora tomentosa and Erythrina speciosa (Fabaceae) seeds. The physical dormancy of seeds has been poorly studied in species from tropical forests, such as the Atlantic Forest. This study aimed to examine the effect of moderate alternating temperatures on breaking the physical dormancy of seeds, the morphoanatomy and histochemistry of seed coats, and to locate the structure/ region responsible for water entrance into the seed, after breaking the physical dormancy of seeds of two woody Fabaceae (subfamily Faboideae) species that occur in the Brazilian Atlantic Forest: Sophora tomentosa and Erythrina speciosa. To assess temperature effect, seeds were incubated in several temperature values that occur in the Atlantic Forest. For morphological and histochemical studies, sections of fixed seeds were subjected to different reagents, and were observed using light or epifluorescence microscopy, to analyze the anatomy and histochemistry of the seed coat. Treated and non-treated seeds were also analyzed using a scanning electron microscope (SEM) to observe the morphology of the seed coat. To localize the specific site of water entrance, the seeds were blocked with glue in different regions and also immersed in ink. in the present work a maximum temperature fluctuation of 15ºC was applied during a period of 20 days and these conditions did not increase the germination of S. tomentosa or E. speciosa. These results may indicate that these seeds require larger fluctuation of temperature than the applied or/and longer period of exposition to the temperature fluctuation. Blocking experiments water inlet combined with SEM analysis of the structures of seed coat for both species showed that besides the lens, the hilum and micropyle are involved in water absorption in seeds scarified with hot water. in seeds of E. speciosa the immersion of scarified seeds into an aniline aqueous solution showed that the solution first entered the seed through the hilum. Both species showed seed morphological and anatomical features for seed coats of the subfamily Faboideae. Lignin and callose were found around all palisade layers and the water impermeability and ecological role of these substances are discussed in the work. Rev. Biol. Trop. 63 (1): 285-294. Epub 2015 March 01.

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“…In the case of O. macrocalyx, this region is characterized by the fractured hilar slit in recently formed seeds, and by the possible presentation of a lens (Figure 4). These structures have been shown to be important zones for water imbibition in legume seeds (De Paula et al 2012). High temperatures also allow degradation of lipids in the seed coat, facilitating water permeability (Zeng et al 2005), a process that is further assisted by the thinner tissue present in this seed region (Varela & Albornoz 2013).…”

Section: Discussionmentioning

confidence: 99%

Seed germination in Ormosia macrocalyx, an endangered tropical forest tree

et al. 2017

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Background: Ormosia macrocalyx is a tropical forest tree classified as endangered. Its seeds experience problems of dispersion and apparent physical dormancy due to their hard seed coating. Hypotheses: 1) The stages of dehiscence of the fruits of Ormosia macrocalyx influence the germinative behavior of its seeds. 2) Pregerminative treatments will improve the germination process of the seeds stored under refrigeration. Study species: Ormosia macrocalyx Study site and period: Villahermosa, Tabasco, Mexico from October 2012 to October 2014 Methods: Two completely randomized experiments were conducted in order to analyze the germination process by 1) evaluating germination in seeds from fruit at three stages of dehiscence (closed, semi-open, and open fruit) and 2) applying four treatments, including three pregerminative treatments (water soaking for 24 h, mechanical scarification and scarification+1% gibberellic acid) plus an untreated control, to seeds stored under refrigeration for 17 months. Results: Differences were found in germination rate (GR), corrected germination rate (CGR) and time to attain 50 % germination (T 50 ) among treatments in the first experiment, with the seeds from open fruits presenting the lowest response (3.31 % day -1 for GR and CGR, and 15.8 days for T 50 ), although all treatments showed similar times for the initiation of germination (GI) and final germination percentage (GP). In the second experiment, the effect of scarification was greater than both the control and soaking treatments. Scarification treatment values were 68.0 % (GP), 12.0 days (GI), 4.53 % day -1 (GR), 6.65 (CGR) and 14.5 days (T 50 ). Addition of gibberellic acid did not produce any further advantage over scarification alone. Conclusions:Seeds from open fruits have lower germination rates, although the final GP is not affected. Stored seeds present physical dormancy but lose viability with storage and, when collected after remaining attached to the tree for undefined periods, scarification can greatly improve their germination rate. Key words: fruit maturation, dormancy, pregerminative treatments, storage Germinación de semillas de Ormosia macrocalyx, un árbol forestal amenazado ResumenAntecedentes: Ormosia macrocalyx es un árbol tropical amenazado. Sus semillas tienen problemas de dispersión y aparente latencia física por cubierta seminal dura. Hipótesis: 1) Las etapas de dehiscencia de los frutos de Ormosia macrocalyx repercutirán en el comportamiento germinativo de sus semillas. 2) Los tratamientos pregerminativos mejorarán el proceso germinativo de las semillas almacenadas en refrigeración Especie de estudio: Ormosia macrocalyx Ducke. Sitio de estudio y fechas: Villahermosa, Tabasco, México, octubre 2012-octubre 2014 Métodos: Se establecieron dos experimentos completamente aleatorios para analizar el proceso de la germinación: 1) Evaluación de la germinación en semillas de frutos en tres estados de madurez (cerrados, semi-abiertos y abiertos), y 2) Aplicación de cuatro tratamientos, tres pregerminativos (r...

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Breaking physical dormancy of Cassia leptophylla and Senna macranthera (Fabaceae: Caesalpinioideae) seeds: water absorption and alternating temperatures

Cited by 45 publications

References 30 publications

Dormición física y conservación ex situ de semillas de Amburana cearensis y Myroxylon peruiferum (Fabaceae)

Dormición física y conservación ex situ de semillas de Amburana cearensis y Myroxylon peruiferum (Fabaceae)

Water absorption and dormancy-breaking requirements of physically dormant seeds of Sophora tomentosa L. and Erythrina speciosa Andr. (Fabaceae-Faboideae)

Seed germination in <em>Ormosia macrocalyx</em>, an endangered tropical forest tree

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