Breaking bud dormancy in Erythronium japonicum Decne. (Liliaceae) by natural and artificial chilling

Kim, Shin Young; Lee, Seung Youn; Rhie, Yong Ha; Kim, Ki Sun

doi:10.1007/s13580-014-0030-9

Cited by 8 publications

(4 citation statements)

References 18 publications

(17 reference statements)

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“…A requirement of chilling exposure for early dormancy release has been shown in perennial tree buds (Arora et al, 2003) and bulb buds of Gladiolus (Bhujbal et al, 2014), Liliaceae (Kim et al, 2014; Langens‐Gerrits et al, 2003) and onions (Benkeblia & Shiomi, 2004). We discovered how to distinguish between ED and ecodormancy in potato tuber buds and found that chilling treatments shorten ED duration (Figures 1 and 2; Figures ).…”

Section: Discussionmentioning

confidence: 99%

Chilling induces sugar and ABA accumulation that antagonistically signals for symplastic connection of dormant potato buds

Danieli

Assouline²,

Salam

et al. 2023

Plant Cell & Environment

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Endodormancy (ED) is a crucial stage in the life cycle of many perennial plants. ED release requires accumulating a certain amount of cold exposure, measured as chilling units. However, the mechanism governing the effect of chilling on ED duration is poorly understood. We used the potato tuber model to investigate the response to chilling as associated with ED release. We measured the accumulation of specific sugars during and after chilling, defined as sugar units. We discovered that ED duration correlated better with sugar units accumulation than chilling units. A logistic function was developed based on sugar units measurements to predict ED duration. Knockout or overexpression of the vacuolar invertase gene (StVInv) unexpectedly modified sugar units levels and extended or shortened ED, respectively. Silencing the energy sensor SNF1‐related protein kinase 1, induced higher sugar units accumulation and shorter ED. Sugar units accumulation induced by chilling or transgenic lines reduced plasmodesmal (PD) closure in the dormant bud meristem. Chilling or knockout of abscisic acid (ABA) 8′‐hydroxylase induced ABA accumulation, in parallel to sweetening, and antagonistically promoted PD closure. Our results suggest that chilling induce sugar units and ABA accumulation, resulting in antagonistic signals for symplastic connection of the dormant bud.

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Section: Discussionmentioning

confidence: 99%

Chilling induces sugar and ABA accumulation that antagonistically signals for symplastic connection of dormant potato buds

Danieli

Assouline²,

Salam

et al. 2023

Plant Cell & Environment

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show abstract

“…A cold-exposure requirement for early dormancy release has been shown in perennial tree buds 49 and bulb buds of Gladiolus 50 , Liliaceae 51,52 and onions 53 . Cold storage is widely used as a tool to delay the sprouting of postharvest potatoes, mainly by maintaining ecodormancy following ED release 54 .…”

Section: Discussionmentioning

confidence: 99%

Sugar units, not chilling units, control endodormancy duration in plants – potato tuber as a case study

Danieli

Assouline²,

et al. 2021

Preprint

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Endodormancy (ED) is a crucial stage in the life cycle of many perennial plants, regulated by genetic and environmental factors. Chilling units, growth regulators, and nutrient supply are considered inducers of ED release, but the mechanism governing ED duration is poorly understood. The potato tuber has been used as a model system to study metabolic processes associated with ED release. Cold-induced sweetening is a well-known response of the tuber to chilling. Here, we found that cold stress induces an increase in sugar units in association with plasmodesmatal closure in the dormant bud cells. Tuber sweetening was associated with shortened ED duration after cooling. Heat exposure also caused sugar unit accumulation followed by faster ED release. A logistic function was developed to predict ED duration based on sugar unit measurements. We discovered that ED release is better correlated with the accumulation of sugar units compared to chilling units. CRISPR/Cas9 knockout of the vacuolar invertase gene (StVInv) induced longer ED, but only in cultivars in which the mutation modified the level of sugar units. Our results suggest that sugar units are better predictors of vegetative bud ED duration than chilling units.

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“…Its vernal characteristics and florescence in early spring make it a perfect ornamental plant. In ephemeral spring plants, flower buds are usually initiated before dormancy induction and continue during the dormancy period [4]. Many studies have been conducted to understand the life cycle, growth habits, reproduction, morphological distinctions, and environmental dynamics in E. japonicum [2,3,[5][6][7][8].…”

Section: Introductionmentioning

confidence: 99%

Transcriptomic Insight into Underground Floral Differentiation in Erythronium japonicum

Wang

Zhang

Shen

et al. 2022

BioMed Research International

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Erythronium japonicum Decne (Liliaceae) flowers in early spring after overwintering. Its sexual reproduction process includes an underground development process of floral organs, but the underlying molecular mechanisms are obscure. The present study is aimed at exploring the transcriptional changes and key genes involved at underground floral developmental stages, including flower primordium differentiation, perianth differentiation, stamen differentiation, and pistil differentiation in E. japonicum. Multistage high-quality transcriptomic data resulted in identifying putative candidate genes for underground floral differentiation in E. japonicum. A total of 174,408 unigenes were identified, 28,508 of which were differentially expressed genes (DEGs) at different floral developmental stages, while only 44 genes were identified with conserved regulation between different stages. Further annotation of DEGs resulted in the identification of 270 DEGs specific to floral differentiation. In addition, ELF3, PHD, cullin 1, SE14, ZSWIM3, GIGNATEA, and SERPIN B were identified as potential candidate genes involved in the regulation of floral differentiation. Besides, we explored transcription factors with differential regulation at different developmental stages and identified bHLH, FAR1, mTERF, MYB-related, NAC, Tify, and WRKY TFs for their potential involvement in the underground floral differentiation process. Together, these results laid the foundation for future molecular works to improve our understanding of the underground floral differentiation process and its genetic regulation in E. japonicum.

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Breaking bud dormancy in Erythronium japonicum Decne. (Liliaceae) by natural and artificial chilling

Abstract: This study was conducted to investigate flower bud differentiation and to determine the chilling requirements for bud dormancy breaking and sprouting in Erythronium japonicum Decne. (Liliaceae). Dormant bulbs grown in an open field were randomly selected from

Cited by 8 publications

References 18 publications

Chilling induces sugar and ABA accumulation that antagonistically signals for symplastic connection of dormant potato buds

Chilling induces sugar and ABA accumulation that antagonistically signals for symplastic connection of dormant potato buds

Sugar units, not chilling units, control endodormancy duration in plants – potato tuber as a case study

Transcriptomic Insight into Underground Floral Differentiation in Erythronium japonicum

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