Breakdown products of C′3 in human synovial fluids

Analyses of CH50, complement components, properdin factors, and kininogen in synovial fluid of patients with juvenile rheumatoid arthritiq revealed evidence of activation of the classic complement pathway in all clinical subgroups. Juveniles with the adult pattern of disease had the greatest incidence of complement abnormalities. Evaluation of individual components by both activity determination and protein measurement showed decreased From the

Section: Discussionmentioning

confidence: 86%

Intraarticular activation of the complement system in patients with juvenile rheumatoid arthritis

Rynes

Ruddy

Spragg

et al. 1976

“…In synovial fluid, local synthesis of C3 has been suggested but its magnitude remains uncertain (39). Activated split products of C3 remain in synovial fluid and can be detected with a variety of techniques (6,7,9,40). Although these split products are nonfunctional in a hemolytic assay (9), they are measured as immunoreactive C3 and thus interfere with estimates of native C3 to the variable degree that a given reagent antisera will cross react.…”

Section: Discussionmentioning

confidence: 99%

Immunoelectrophoretic assay for synovial fluid C3 with correction for synovial fluid globulin

Hasselbacher

1979

Synovial fluid C3 was measured by electroimmunoassay. When C3 was expressed as mg/ml, the amounts found in Reiter's disease, psoriatic arthritis, gout, and systemic lupus erythematosus were significantly different from degenerative arthritis. When C3 was corrected for total protein, the levels for rheumatoid arthritis, Reiter's disease, psoriatic arthritis, and systemic lupus were significantly different from degenerative arthritis. When C3 was corrected for synovial fluid globulin, only rheumatoid arthritis and systemic lupus were significantly different from degenerative arthritis. Correction of C3 for globulin increases the difference between rheumatoid arthritis and degenerative arthritis. A proportion of gouty fluids with a relative decrease in C3 is demonstrated. It is argued that correction of C3 for globulin is more meaningful than correction for total

“…Further evidence for the activation of late components including a reduction of C9 in seropositive patients has been noted more recently (29). Evidence for local activation of C3 includes the demonstration of an anodal activation fragment of C3 in rheumatoid synovial fluids (30). Chemotactic activity in rheumatoid synovial fluids has been related to the presence of both C5a and C m (31).…”

Section: Discussionmentioning

confidence: 99%

Synovial fluid lymphocytes in rheumatoid arthritis

Utsinger

1975

There is significant evidence implicating immunologic mechanisms in the pathogenesis of rheumatoid arthritis (RA). To investigate the possibility that a cellular imbalance of T and B lymphocytes plays a role in perpetuating synovitis, an examination of the peripheral blood and synovial fluid lymphocytes of patients with seropositive rheumatoid arthritis was undertaken. A significant lymphopenia was encountered in RA patients, with values approximately midway between those in normal controls and the severe lymphopenia seen in a group of patients with systemic lupus erythematosus. A significantly greater diminution of T than B cells was noted in RA, and normal percentage distributions of lymphocytes in peripheral blood were documented. Normal percentages of T and B cells, similar to those in peripheral blood, were found in synovial fluids of patients with seropositive RA, seronegative RA, osteoarthritis, and gout. Two seropositive patients ex-