Breadfruit (Artocarpus altilis) DELLA genes: gibberellin-regulated stem elongation and response to high salinity and drought

Zhou, Yuchan; Underhill, Steven J. R.

doi:10.1007/s10725-017-0302-3

Cited by 11 publications

(13 citation statements)

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“…Two DELLA genes, AaDELLA1 and AaDELLA2, were previously cloned from "Mason" breadfruit [30]. To confirm that the two genes or potentially more DELLA genes were present in stems of "Gold Noli" breadfruit, DELLA genes were first cloned from "Gold Noli" breadfruit by degenerate PCR using primers corresponding to two conserved regions of all known DELLAs, MDELLA(V/A) and AHFTANQA [23].…”

Section: Effect Of Rootstocks On Della Protein Abundance and Transcrimentioning

confidence: 99%

“…Total RNA was extracted from stem tissues of breadfruit plants by using RNeasy kit RNeasy kit (Qiagen, VIC, Australia) and reverse transcribed with reverse transcriptase and oligo(dT) (Life Technologies, VIC, Australia). The resulting cDNA was subjected to degenerate PCR using primers 5 -ATGGAYGARYTIYTIGCNG-3 and 5 -GCNCAYTTYACNGCNAAYCARGCN-3 as previously described [30]. The PCR reactions were performed at 35 cycles with annealing temperature at 52 • C. The PCR products were cloned into pGEMT vector (Promega, NSW, Australia) and sequenced.…”

Section: Cloning Of Della Cdnas From "Gold Noli" Breadfruitmentioning

confidence: 99%

“…A cohort of four GA2-oxidase genes, AaGA2ox1-AaGA2ox4, was also cloned in the same cultivar, with three highly expressed in vegetative organs [29]. There are two DELLA genes, AaDELLA1 and AaDELLA2, isolated in "Mason" breadfruit species [30]. However, the lack of dwarf varieties and the scarcity of dwarfing rootstocks for breadfruits has limited our understanding of how vegetative growth is controlled by rootstocks in the species and any potential role of these genes in conferring breadfruit dwarfism.…”

Section: Introductionmentioning

confidence: 99%

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Expression of Gibberellin Metabolism Genes and Signalling Components in Dwarf Phenotype of Breadfruit (Artocarpus altilis) Plants Growing on Marang (Artocarpus odoratissimus) Rootstocks

Zhou

Underhill

2020

Plants

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Breadfruit (Artocarpus altilis) is a traditional staple tree crop throughout the tropics. The species is an evergreen tree 15–20 m; there are currently no size-controlling rootstocks within the species. Through interspecific grafting, a dwarf phenotype was identified in breadfruit plants growing on Marang (Artocarpus odoratissimus) rootstocks, which displayed ~60% reduction in plant height with ~80% shorter internodes. To gain insight into the molecular mechanism underlying rootstock-induced dwarfing, we investigated the involvement of gibberellin (GA) in reduction of stem elongation. Expression of GA metabolism genes was analysed in the period from 18 to 24 months after grafting. In comparison to self-graft and non-graft, scion stems on marang rootstocks displayed decrease in expression of a GA biosynthetic gene, AaGA20ox3, and increase in expression of a GA catabolic genes, AaGA2ox1, in the tested 6-month period. Increased accumulation of DELLA proteins (GA-signalling repressors) was found in scion stems growing on marang rootstocks, together with an increased expression of a DELLA gene, AaDELLA1. Exogenous GA treatment was able to restore the stem elongation rate and the internode length of scions growing on marang rootstocks. The possibility that GA deficiency forms a component of the mechanism underlying rootstock-induced breadfruit dwarfing is discussed.

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Section: Effect Of Rootstocks On Della Protein Abundance and Transcrimentioning

confidence: 99%

Section: Cloning Of Della Cdnas From "Gold Noli" Breadfruitmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Expression of Gibberellin Metabolism Genes and Signalling Components in Dwarf Phenotype of Breadfruit (Artocarpus altilis) Plants Growing on Marang (Artocarpus odoratissimus) Rootstocks

Zhou

Underhill

2020

Plants

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“…AtABI5 in Arabidopsis functions as the main transcription factor in abscisic acid signal transduction [34,35]. Thus, MdABI5 may act to integrate gibberellins and abscisic acid signals with dwar ng effect by interacting with MdG3OX3, INDUCER OF CBF EXPRESSION1 (ICE1), and DELLA protein [11,36].…”

Section: Discussionmentioning

confidence: 99%

“…rootstock, the low root zeatin content, caused by the lower expression of MdIPT gene encoding isopentenyl transferase, contributes to the weakened vegetative growth of the scion that has been grafted onto 'M9' rootstock [10]. Gibberellins (GA) has been discovered as a rate-limiting hormone in the process of dwar ng control of plants such as apples (Malus pumila Mill), breadfruits (Artocarpus altilis) and bean (Phaseolus coccineus) [11][12][13][14].…”

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confidence: 99%

Development and validation of functional markers from Dw1 and Dw2 loci to accurately predict apple rootstock dwarfing ability

Yang

Shen²,

Liu

et al. 2020

Preprint

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Background: ‘M9’ is a widely used apple dwarfing rootstock due to the outstanding effects on both precocity and vigorous control. Two quantitative trait loci (QTLs), Dw1 and Dw2 , for the dwarfing effect were previously mapped on ‘M9’, but the genetic variations that underpin the dwarfing ability have not been elucidated to date. Result: By using ‘Red Fuji’ trees grafted on 1123 hybrids from ‘ M alus baccata ’ × ‘M9’, the intervals of Dw1 and Dw2 were narrowed down. MdLBD3 and MdARF6 were predicted as potential candidate genes from Dw1 , while MdG3OX3 was a possible candidate gene from Dw2 . An 11 bp deletion at -339 bp upstream of the transcription start site (TSS) of MdLBD3 generated a new cis-element binding site with MdWRKY2 and caused increased expression of MdLBD3 . Coincidently, a ten bp deletion at -278 bp upstream of the TSS of MdG3OX3 created an additional binding site of MdABI5 , leading to higher expression of MdG3OX3 . At -954 bp of the MdARF6 promoter, a 14 bp insertion destroyed the binding ability by MdABI5 and reduced MdARF6 expression. The genotype effects of these insertion and deletions as diagnostic markers on dwarfing traits (tree height, trunk diameter, and canopy width) were estimated in 108 F1 hybrids. The genomic predicted genetic values (GEGV) were calculated by adding up the genotype effects of the three markers and the population mean phenotype. The GEGV of the dwarfing traits exhibited high correlation coefficients of 0.93, 0.94, and 0.93 in terms of tree height, trunk diameter, and canopy width for the observed phenotype values, respectively. The predictability of GEGV was validated in 64 Malus accessions. Conclusion : The development of the three functional markers, Ld/Li, Ad/Ai, and Gd/Gi, ensures the accurate genomic assisted prediction of dwarfing ability in apple rootstock breeding. The data also suggested that ABA, auxin, GA, and zeatin signals may be involved in the regulation of apple rootstock dwarfing mechanism.

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Grow Us Our Daily Bread

Lincoln

Ragone

Zerega

et al. 2018

Horticultural Reviews

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Breadfruit (Artocarpus altilis) DELLA genes: gibberellin-regulated stem elongation and response to high salinity and drought

Cited by 11 publications

References 47 publications

Expression of Gibberellin Metabolism Genes and Signalling Components in Dwarf Phenotype of Breadfruit (Artocarpus altilis) Plants Growing on Marang (Artocarpus odoratissimus) Rootstocks

Expression of Gibberellin Metabolism Genes and Signalling Components in Dwarf Phenotype of Breadfruit (Artocarpus altilis) Plants Growing on Marang (Artocarpus odoratissimus) Rootstocks

Development and validation of functional markers from Dw1 and Dw2 loci to accurately predict apple rootstock dwarfing ability

Grow Us Our Daily Bread

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