BRD7, a novel bromodomain gene, inhibits G1–S progression by transcriptionally regulating some important molecules involved in ras/MEK/ERK and Rb/E2F pathways

Abstract

Bromodomain is a 110 amino acid domain. It is evolutionally conserved and is found in proteins strongly implicated in signal-dependent transcriptional regulation. BRD7 is a novel bromodomain gene and it is downexpressed in nasopharyngeal carcinoma (NPC) biopsies and cell lines; its function is poorly understood. In the present study, tet-on inducible expression system was used to investigate the role of BRD7 in cell growth and cell cycle progression. We found that ectopic expression of BRD7 in NPC cells inhibited cell growth and cell cycle progression from G1 to S. We further performed cell cycle cDNA array to screen potential transcriptional targets of BRD7 in cell cycle. Thirteen important signaling molecules, mainly implicated in ras/MEK/ERK and Rb/E2F pathways, were differentially expressed by induction of BRD7. Moreover, we observed that BRD7 could regulate the promoter activity of E2F3, one of its targets. Taken together, the present study indicated that BRD7 inhibited G1-S progression by transcriptionally regulating some important molecules involved in ras/MEK/ERK and Rb/E2F pathways and suggested that BRD7 may present a promising candidate of NPC trade mark associated tumor suppressor gene.

A Sex-Specific Role of Type VII Adenylyl Cyclase in Depression

“…BRD7 is a novel bromodomain gene that is believed to be involved in signal-dependent transcriptional regulation. Recent data suggest that BRD7 may play a role in certain cancers (Zhou et al, 2004), yet there is no evidence to suggest that it is involved in the etiology of any psychiatric disorder.…”

Preparation of Polyclonal Antibody Specific for BRD7 and Detection of Its Expression Pattern in the Human Fetus

“…Staal et al (2000) reported that BRD7 protein (celtix-1) interacts with interferon regulatory factor 2 in the nucleus and associates with transcriptionally active chromatin in situ. Overexpression of the BRD7 gene in NPC cells is effective to inhibit cell growth and cell cycle progression from G1 to S phase by transcriptional regulation of some cell cycle-related genes (Yu et al 2001;Zhou et al 2004;Peng et al 2006). To better understand the cellular role of the BRD7 gene, in this study, we explored an approach to generate a highly specific BRD7 antibody, and showed that the prepared BRD7 antibody is able to specifically recognize recombinant GST-BRD7N protein and endogenously expressed BRD7 protein.…”

Signaling pathway in development of Camellia oleifera nurse seedling grafting union

“…Cell proliferation was promoted by the inhibition of BRD activity in response to calcium signal stimulation (Zhou et al 2004;Korner and Tibes 2008;Sterner et al 1999). It is qualified by the presence of two indicators (CDPK, positive contribution; BRD, negative) in PC-1 (Table 3).…”

“…These indicated that PC-1 (Table 1) and PC-1 (Table 3) were related to the development of the vascular bundle of grafting union. The 7 main indicators of the 17 main indicators in PC-1 (Table 1) and PC-1 (Table 3) (41%) directly were related with calcium signal [POD (Erinle et al 2016), cellulose content (Lin et al 2016), chlorogenic acid content (Ngadze et al 2014), CDPK (Fu et al 2013), CHI , GST (Dulhunty et al 2001), and BRD (Zhou et al 2004)]. Therefore, after grafting, vascular bundle development at the scion may depend on biological responses in which intracellular calcium acts as a second messenger.…”