BRCC2 inhibits breast cancer cell growth and metastasis in vitro and in vivo via downregulating AKT pathway

Li, Xiao Xing; Kong, Xiangnan; Wang, Y; Yang, Qifeng

doi:10.1038/cddis.2013.290

Cited by 20 publications

(24 citation statements)

References 42 publications

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“…Recent studies have revealed that miRNAs act as an important regulator of gene expression at the post-transcriptional level and regulates a wide range of physiological and developmental processes[ 16 – 19 ]. Over the past several years, it has become clear that deregulated of miRNAs contribute to the development of most human cancers such as gastric cancer, bladder cancer and breast cancer, which they act as either oncogenes or tumor suppressors[ 20 – 23 ]. In the present study, we investigated the role of miR-377 in human HCC development.…”

Section: Discussionmentioning

confidence: 99%

MicroRNA-377 Suppresses Cell Proliferation and Invasion by Inhibiting TIAM1 Expression in Hepatocellular Carcinoma

Chen

Liu

et al. 2015

PLoS ONE

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Increasing evidence has suggested that microRNAs (miRNAs) play an important role in the initiation and progression of hepatocellular carcinoma (HCC). Here, we identified a novel tumor suppressive miRNA, miR-377, and investigated its role in HCC. The expression of miR-377 in HCC tissues and cell lines was detected by real-time reverse-transcription PCR. The effects of miR-377 on HCC cell proliferation and invasion were also investigated. Western blot and luciferase reporter assay were used to identify the direct and functional target of miR-377. The expression of miR-377 was markedly downregulated in human HCC tissues and cell lines. MiR-377 can dramatically inhibit cell growth and invasion in HCC cells. Subsequent investigation revealed that T lymphoma invasion and metastasis 1 (TIAM1) was a direct and functional target of miR-377 in HCC cells. Overexpression of miR-377 impaired TIAM1-induced promotion of proliferation and invasion in HCC cells. Finally, miR-377 is inversely correlated with TIAM1 expression in human HCC tissues. These findings reveal that miR-377 functions as a tumor suppressor and inhibits the proliferation and invasion of HCC cells by targeting TIAM1, which may consequently serve as a therapeutic target for HCC patients.

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Section: Discussionmentioning

confidence: 99%

MicroRNA-377 Suppresses Cell Proliferation and Invasion by Inhibiting TIAM1 Expression in Hepatocellular Carcinoma

Chen

Liu

et al. 2015

PLoS ONE

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“…Previous work has suggested that ILK regulates cell survival through the PI3K/Akt pathway (32), which has also been implicated in cancer cell (33,34) and CSC survival (35,36), cancer cell proliferation (37,38), and the CSC phenotype (39). We found that knockdown of ILK reduced the phosphorylation of Akt (Figure 4A; Figure S4).…”

Section: Resultsmentioning

confidence: 99%

Tissue Stiffness and Hypoxia Modulate the Integrin-Linked Kinase ILK to Control Breast Cancer Stem-like Cells

et al. 2016

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Breast tumors are stiffer and hypoxic compared to nonmalignant breast tissue. Here we report that stiff and hypoxic microenvironments promote the development of breast cancer stem-like cells (CSC) through modulation of the integrin-linked kinase ILK. Depleting ILK blocked stiffness and hypoxia-dependent acquisition of CSC marker expression and behavior, whereas ectopic expression of ILK stimulated CSC development under softer or normoxic conditions. Stiff microenvironments also promoted tumor formation and metastasis in ovo, where depleting ILK significantly abrogated the tumorigenic and metastatic potential of invasive breast cancer cells. We further found that the ILK-mediated phenotypes induced by stiff and hypoxic microenvironments are regulated by PI3K/Akt. Analysis of human breast cancer specimens revealed an association between substratum stiffness, ILK and CSC markers, insofar as ILK and CD44 co-localized in cancer cells located in tumor regions predicted to be stiff. Our results define ILK as a key mechanotransducer in modulating breast CSC development, in response to tissue mechanics and oxygen tension.

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“…Among the miRNAs without previously reported association and whose biological functions have not been characterized in PCa are hsa-miR-1973, hsa-miR-575, hsa-miR-630 and hsa-miR-600. hsa-miR-575 and the hsa-miR-630 are proposed oncomiRNAs in other tumors such as gastric cancer, lung cancer, renal cell carcinoma, hepatocellular carcinoma, and breast cancer [34][35][36][37][38][39][40]. hsa-miR-630 has different gene targets such as BCL-2, MTDH, YAP-1, SNAI2 [37][38][39] involved in PCa oncogenesis [41][42][43][44].…”

Section: Discussionmentioning

confidence: 99%

“…hsa-miR-575 has as a target BLID (BH3-like motif containing, BRCC2). BLID is a tumor-suppressor gene involved in DNA repair and gene integrity [40]. In breast cancer, BLID inhibited cancer cell growth and metastasis via downregulating AKT pathway [40].…”

Section: Discussionmentioning

confidence: 99%

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microRNA Expression Profiling in Young Prostate Cancer Patients

et al. 2020

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MicroRNAs (miRNAs) are small, non-coding RNA molecules with multiple roles in many biological processes. Few studies have shown the molecular characteristics in younger prostate cancer (PCa) patients. In this study, we performed miRNA profiling in young PCa (EO-PCa) cases compared with PCa arising in older men (LO-PCa). Experimental Design: Formalin-fixed, paraffin embedded tissue was used. miRNA was extracted for PCR array and NanoString methods. Relative miRNAs expression levels were obtained by comparing young vs older men, and young PCa tumor samples vs normal epithelium. Results: miRNA profiling showed a different expression pattern in PCa arising in younger men, and young PCa tumoral and its normal counterpart. Nine miRNAs showed differences in the expression compared to LO-PCa. Fourteen miRNAs were significantly up-regulated young PCa tumoral tissue compared to normal counterpart. The higher expression of miR-600 and miR-137 were associated with high Gleason score, extraprostatic extension and lymphatic invasion. Conclusion: These results suggest that PCa in younger patients has a different expression profile compared to normal tissue and PCa arising in older man. Differentially expressed miRNAs provide insights of molecular mechanisms involve in this PCa subtype.

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BRCC2 inhibits breast cancer cell growth and metastasis in vitro and in vivo via downregulating AKT pathway

Cited by 20 publications

References 42 publications

MicroRNA-377 Suppresses Cell Proliferation and Invasion by Inhibiting TIAM1 Expression in Hepatocellular Carcinoma

MicroRNA-377 Suppresses Cell Proliferation and Invasion by Inhibiting TIAM1 Expression in Hepatocellular Carcinoma

Tissue Stiffness and Hypoxia Modulate the Integrin-Linked Kinase ILK to Control Breast Cancer Stem-like Cells

microRNA Expression Profiling in Young Prostate Cancer Patients

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