Brazilin isolated from Caesalpinia sappan L. inhibits rheumatoid arthritis activity in a type-II collagen induced arthritis mouse model

Jung, Eui‐Man; Han, Kook-Il; Hwang, Seon Gu; Kwon, Hyun-Jung; Patnaik, Bharat Bhusan; Kim, Yong Hyun; Han, Man-Deuk

doi:10.1186/s12906-015-0648-x

Cited by 41 publications

(34 citation statements)

References 42 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Isolation of the major compound from F-EtOH was done by using silica gel column chromatography. The obtained isolated compound was confirmed to be brazilin by comparing the obtained 1 H NMR and 13 C NMR spectra as well as the supporting data of MS and UV with the data previously reported in the literatures (11,15,21,22). Considering the HPLC chromatogram of F-EtOH, it confirmed that F-EtOH contained not only brazilin but also one obvious minor peak and the other two tiny peaks.…”

Section: Discussionsupporting

confidence: 78%

“…The heartwood part of C. sappan can produce natural red dye that can be used as coloring agent in food, beverage, and cosmetics. For biological properties, it has been reported to have antioxidant (8,9), anti-inflammation (10), anti-rheumatoid arthritis (11) and antimicrobial activity (12)(13)(14). Many phenolic compounds, such as xanthone, coumarin, chalcones, flavones, and homoisoflavonoids have been found from C. sappan (15).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

<i>Caesalpinia sappan</i>: A promising natural source of antimicrobial agent for inhibition of cariogenic bacteria

Puttipan

Chansakaow

Khongkhunthian

et al. 2018

DD&T

View full text Add to dashboard Cite

From the previous findings, the ethanolic fractionated extract of Caesalpinia sappan (F-EtOH) has high activity against Streptococcus mutans, the most severe cariogenic bacteria. The present study was aimed to isolate and identify the active compound of F-EtOH and compare its inhibitory activity against the biofilm of S. mutans as well as the cytotoxicity to oral fibroblast cells with F-EtOH. Compound isolation was done by column chromatography. The active compound was identified using liquid chromatography-mass spectrometry with electrospray ionization and nuclear magnetic resonance spectroscopy. It was found that the major compound of F-EtOH is brazilin. F-EtOH and brazilin were compared for inhibitory potential on the biofilms of three strains of S. mutans. The results exhibited that both F-EtOH and brazilin had potential on inhibiting biofilm formation and eradicating the preformed biofilms and their activity was dose dependent. F-EtOH showed significantly less toxic to normal periodontal ligament fibroblast than brazilin. At low concentration of 1- and 2-MBC, F-EtOH showed higher effective than brazilin. The results of our study suggest that the antibacterial activity of F-EtOH is according to the synergistic effects of the existing compounds including brazilin in F-EtOH.

show abstract

Section: Discussionsupporting

confidence: 78%

Section: Introductionmentioning

confidence: 99%

<i>Caesalpinia sappan</i>: A promising natural source of antimicrobial agent for inhibition of cariogenic bacteria

Puttipan

Chansakaow

Khongkhunthian

et al. 2018

DD&T

View full text Add to dashboard Cite

show abstract

“…Applied in animal models of RA, daily oral treatment with CSE markedly attenuated collagen‐induced arthritis (CIA) and reduced the levels of IL‐1β, IL‐6, TNF‐α and PGE2 in serum and the expression of COX‐2 and NF‐κB/p65 in paw cartilage . Also, intraperitoneal administration of brazilin isolated from C. sappan was found to markedly attenuate CIA in mice and to reduce the serum levels of inflammatory cytokines . In mice, brazilin was also reported to have therapeutic potential in preventing bone loss …”

Section: Discussionmentioning

confidence: 99%

Brazilin blocks catabolic processes in human osteoarthritic chondrocytes via inhibition of NFKB1/p50

Weinmann

Mueller

Walzer

et al. 2018

Journal Orthopaedic Research

View full text Add to dashboard Cite

This study aimed to evaluate the chondroprotective and anti-inflammatory activity of brazilin in human osteoarthritic (OA) cartilage and chondrocytes with particular focus on the nuclear factor-kappa B (NF-κB) pathway. Therefore, brazilin was isolated from Caesalpinia sappan and identified using high performance liquid chromatography (HPLC). The effect of brazilin was assessed in cartilage explants treated with 10 ng/ml interleukin (IL)-1β and 10 ng/ml tumor necrosis factor (TNF)-α using histological and biochemical glycosaminoglycan (GAG) analyses and in primary chondrocytes treated with 10 ng/ml IL-1β using RT-qPCR, ELISA, and Western blot. The involvement of NF-κB signaling was examined using a human NF-κB signaling array and in silico pathway analysis. Brazilin was found to reduce the GAG loss from cartilage explants stimulated with IL-1β and TNF-α. NF-κB pathway analysis in chondrocytes revealed NFKB1/p50 as a central player regulating the anti-inflammatory activities of brazilin. Brazilin suppressed the IL-1β-mediated up-regulation of OA markers and the induction of NFKB1/p50 in chondrocytes. In conclusion, brazilin effectively attenuates catabolic processes in human OA cartilage and chondrocytes-at least in part due to the inhibition of NFKB1/p50-which indicates a chondroprotective potential of brazilin in OA. © 2018 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 36:2431-2438, 2018.

show abstract

“…The CIA model was induced using 2‐month‐old mice of DBA/1 strain as previously described . The vehicle group was injected with equal volumes of PSB at the same time.…”

Section: Methodsmentioning

confidence: 99%

Early supplemental α2‐macroglobulin attenuates cartilage and bone damage by inhibiting inflammation in collagen II‐induced arthritis model

Chen

Wei

et al. 2019

Int J of Rheum Dis

View full text Add to dashboard Cite

Objective:To determine if early supplemental intra-articular α2-macroglobulin (A2M) has a chondroprotective effect in a collagen II-induced arthritis (CIA) mice model. Methods: DBA/1 mice were randomized into four groups (n = 15/group): (a) CIA + 1.2 μg of A2M; (b) CIA + 0.8 μg of A2M; (c) CIA + 0.4 μg of A2M; (d) vehicle + phosphate-buffered saline (PBS). A2M was injected into right ankles and PBS was injected into the left ankles simultaneously as internal control at days 36, 43 and 50. The CIA inflammation clinical score and ankle thickness were recorded every other day starting on day 21 until sacrifice. Changes in inflammation were monitored by in vivo fluorescence molecular tomography (FMT). Inflammation, cartilage and bone damage were assessed with X-ray, histology and immunohistochemistry.Cartilage and inflammation-related gene expression was quantified by real-time polymerase chain reaction (PCR). Results:All mice showed ankle inflammation on day 33. After day 43, lower clinical scores, ankle thickness and Sharp/van der Heijde method scores in A2M-treated ankles compared with PBS-treated ankles. FMT data indicated that the inflammation markers MMPSense and ProSense were significantly elevated in the PBS-treated ankles than A2M-treated ankles. Histology and X-ray analyses indicated that A2M administration resulted in lower levels of inflammatory infiltration and synovial hyperplasia, as well as more typical cartilage and bone organization with increased COL II and Aggrecan staining when compared with PBS-treated ankles. In addition, real-time PCR showed that,matrix metalloproteinase-3, -9, -13, COL X and Runx2 were significantly less expressed in A2M-treated groups than PBS-treated animals. Conclusion:Early supplemental intra-articular A2M exerts an anti-inflammatory effect and attenuates cartilage and bone damage in a CIA model. K E Y W O R D Scartilage and bone damage, CIA, fluorescence molecular tomography, inflammation, α2macroglobulin | 655 LI et aL.

show abstract

Brazilin isolated from Caesalpinia sappan L. inhibits rheumatoid arthritis activity in a type-II collagen induced arthritis mouse model

Cited by 41 publications

References 42 publications

<i>Caesalpinia sappan</i>: A promising natural source of antimicrobial agent for inhibition of cariogenic bacteria

<i>Caesalpinia sappan</i>: A promising natural source of antimicrobial agent for inhibition of cariogenic bacteria

Brazilin blocks catabolic processes in human osteoarthritic chondrocytes via inhibition of NFKB1/p50

Early supplemental α2‐macroglobulin attenuates cartilage and bone damage by inhibiting inflammation in collagen II‐induced arthritis model

Contact Info

Product

Resources

About