Brazilin blocks the induction of NFKB1/P50 in human chondrocytes and synoviocytes

Abstract

chondro-induction). Chondro-induction was lower (1.3AE0.4) and less reproducible when coculture was performed with M14 and did not occur with skin fibroblasts. GAG contents of constructs generated by solely macrophages were undetectable. Histological analyses of constructs confirmed the biochemical results. In the coculture there was no modulation of the chondrogenic genes. As compared to monocultures, in co-culture MSC and M24 numbers decreased less markedly (at day 7, MSC were 84% and 42% of the initial number, M24 were 26% of the initial number and undetectable, respectively for co-cultured and monocultured cells). Conclusions: We have demonstrated that coculture MSC/M42 results in synergistic cartilage tissue formation, which is not mediated by soluble factors alone. Further studies are envisioned to investigate whether M42 modulate the survival of specific MSC sub-populations. Finally, in vivo studies are necessary to assess the clinical relevance of our findings in the context of cartilage repair.