Brazilian green propolis improves gut microbiota dysbiosis and protects against sarcopenic obesity

Okamura, Tomio; Hamaguchi, Masahide; Bamba, Ryo; Nakajima, Harushi; Yoshimura, Yuta; Kimura, Tomonori; Hashimoto, Yoshitaka; Majima, Susumu; Senmaru, Takafumi; Ushigome, Emi; Nakanishi, Naoko; Asano, Mai; Yamazaki, Masahiro; Nishimoto, Yuichiro; Yamada, Takuji; Fujikura, Chizuru; Asama, Takashi; Okumura, Nobuaki; Takakuwa, Hiroshi; Sasano, Ryoichi; Fukui, Michiaki

doi:10.1002/jcsm.13076

Cited by 21 publications

(20 citation statements)

References 45 publications

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“…In this direction, the green propolis produced in the South region of Brazil was the first to be recognized for its antimicrobial potential. Recently, it was found to impair gut microbiota dysbiosis by enhancing the Bacteroidetes/Firmicutes proportion in an animal study ( Okamura et al, 2022 ). In addition, the baccharin, one of its biocompounds, has shown a possible antimicrobial mechanism of action on P. gingivalis .…”

Section: Discussionmentioning

confidence: 99%

Antimicrobial activity of Desplac® oral gel in the subgingival multispecies biofilm formation

Bueno-Silva,

Kiausinus,

Gonçalves

et al. 2023

Front. Microbiol.

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Natural products are well-known due to their antimicrobial properties. This study aimed to evaluate the antimicrobial effect of Desplac® product (composed of Aloe Vera, Propolis Extract, Green Tea, Cranberry, and Calendula) on the subgingival biofilm. Two different protocols were used to treat the 33-species biofilms: (A) 2×/day (12/12 h) for 1 min with Desplac® or Noplak Toothpaste (Chlorhexidine + Cetylpyridinium Chloride) or Oral B ProGengiva (stannous Fluoride) or a placebo gel; (B) a 12-h use of the Desplac® product or 0.12% chlorhexidine gel or a placebo gel. After 7 days of biofilm formation, the metabolic activity (MA) and biofilm profile were determined by 2,3,5-triphenyltetrazolium chloride and Checker-board DNA–DNA hybridization, respectively. Statistical analysis used the Kruskal-Wallis test followed by Dunn’s post-hoc. In protocol A, all treatments presented reduced MA compared to the placebo (p ≤ 0.05). The Desplac®-treated biofilm showed a similar microbial profile to other antimicrobials, although with higher bacterial total counts. In protocol B, MA of Desplac®-treated biofilms was lower than the placebo’s MA but higher than chlorhexidine-treated biofilms (p ≤ 0.05). Pathogen levels in Desplac®-treated biofilms were lower than in placebo-treated biofilms and elevated compared to the chlorhexidine-treated biofilms (p ≤ 0.05). Desplac® inhibited the biofilm development and disrupted the mature subgingival biofilm, highlighting its effect on Tannerella forsythia counts.

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Section: Discussionmentioning

confidence: 99%

Antimicrobial activity of Desplac® oral gel in the subgingival multispecies biofilm formation

Bueno-Silva,

Kiausinus,

Gonçalves

et al. 2023

Front. Microbiol.

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“…In the realm of organic constituents, noteworthy elements include terpenes and steroids, as well as sugars and amino acids, along with various flavonoids. Notable among the flavonoids are caffeic, cinnamic, p-coumaric, and chicoric acids, as well as quercetin, pinocembrin, baicalin, galangin, and chrysin, owing to their significant antiglycation [ 37 , 38 , 39 ], cardioprotective [ 40 , 41 , 42 , 43 , 44 ], neuroprotective [ 45 , 46 , 47 , 48 , 49 , 50 ], cytostatic [ 51 , 52 , 53 ], radioprotective [ 54 , 55 , 56 , 57 ], antimicrobial [ 58 , 59 , 60 , 61 , 62 , 63 ], anticancer [ 64 , 65 , 66 , 67 , 68 , 69 , 70 ], antioxidant [ 71 , 72 , 73 , 74 , 75 , 76 ], immunomodulatory [ 77 , 78 , 79 , 80 , 81 , 82 , 83 ], antidiabetic [ 84 , 85 , 86 , 87 , 88 , ...…”

Section: Propolismentioning

confidence: 99%

“…As a result, this may lead to an indirect reduction in appetite and subsequent food consumption [ 171 ]. Through their interaction with the free fatty acid receptors FFA2 and FFA3, SCFAs effectively prevent nuclear factor kappa B activation, leading to the suppression of tumor necrosis factor α (TNF-α) and interleukin-6 (IL-6) release [ 80 ]. This inhibition is crucial as persistent inflammation can induce insulin resistance in bodily organs, ultimately impairing glucose tolerance.…”

Section: Gut Microbiotamentioning

confidence: 99%

Exploring the Prospective Role of Propolis in Modifying Aging Hallmarks

Scorza,

Goncalves,

Finsterer

et al. 2024

Cells

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Aging populations worldwide are placing age-related diseases at the forefront of the research agenda. The therapeutic potential of natural substances, especially propolis and its components, has led to these products being promising agents for alleviating several cellular and molecular-level changes associated with age-related diseases. With this in mind, scientists have introduced a contextual framework to guide future aging research, called the hallmarks of aging. This framework encompasses various mechanisms including genomic instability, epigenetic changes, mitochondrial dysfunction, inflammation, impaired nutrient sensing, and altered intercellular communication. Propolis, with its rich array of bioactive compounds, functions as a potent functional food, modulating metabolism, gut microbiota, inflammation, and immune response, offering significant health benefits. Studies emphasize propolis’ properties, such as antitumor, cardioprotective, and neuroprotective effects, as well as its ability to mitigate inflammation, oxidative stress, DNA damage, and pathogenic gut bacteria growth. This article underscores current scientific evidence supporting propolis’ role in controlling molecular and cellular characteristics linked to aging and its hallmarks, hypothesizing its potential in geroscience research. The aim is to discover novel therapeutic strategies to improve health and quality of life in older individuals, addressing existing deficits and perspectives in this research area.

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“…Overeating 2 and inactivity 3 are the main causes of type 2 diabetes, and the associated increase in visceral fat and decrease in muscle mass or sarcopenia 4 and obesity are the pathological bases for this disease. We previously reported findings of various human and animal studies on sarcopenic obesity and type 2 diabetes and showed that db/db mice with defects in the leptin receptor, characterized by overeating and inactivity, exhibit sarcopenic obesity 5 . Thus, prevention of sarcopenic obesity is important to prevent the onset and exacerbation of type 2 diabetes.…”

Section: Introductionmentioning

confidence: 99%

“…We previously reported findings of various human and animal studies on sarcopenic obesity and type 2 diabetes and showed that db/db mice with defects in the leptin receptor, characterized by overeating and inactivity, exhibit sarcopenic obesity. 5 Thus, prevention of sarcopenic obesity is important to prevent the onset and exacerbation of type 2 diabetes.…”

Section: Introductionmentioning

confidence: 99%

Milk protects against sarcopenic obesity due to increase in the genus Akkermansia in faeces of db/db mice

Okamura

Hamaguchi

Nakajima

et al. 2023

J cachexia sarcopenia muscle

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Background Sarcopenic obesity, a combination of sarcopenia and obesity, is a pathological feature of type 2 diabetes. Several human studies have shown that milk is useful in the prevention of sarcopenia. This study was aimed at clarifying the effect of milk on the prevention of sarcopenic obesity in db/db mice. Methods A randomized and investigator‐blinded study was conducted using male db/db mice. Eight‐week‐old db/db mice were housed for 8 weeks and fed milk (100 μL/day) using a sonde. The faecal microbiota transplantation (FMT) group received antibiotics for 2 weeks, starting at 6 weeks of age, followed by FMT twice a week until 16 weeks of age. Results Milk administration to db/db mice increased grip strength (Milk−: 164.2 ± 4.7 g, Milk+: 230.2 ± 56.0 g, P = 0.017), muscle mass (soleus muscle, Milk−: 164.2 ± 4.7 mg, Milk+: 230.2 ± 56.0 mg, P < 0.001; plantaris muscle, Milk−: 13.3 ± 1.2 mg, Milk+: 16.0 ± 1.7 mg, P < 0.001) and decreased visceral fat mass (Milk−: 2.39 ± 0.08 g, Milk+: 1.98 ± 0.04 mg, P < 0.001), resulting in a significant increase in physical activity (light: P = 0.013, dark: P = 0.034). FMT from mice fed milk not only improved sarcopenic obesity but also significantly improved glucose intolerance. Microarray analysis of gene expression in the small intestine revealed that the expression of amino acid absorption transporter genes, namely, SIc7a5 (P = 0.010), SIc7a1 (P = 0.015), Ppp1r15a (P = 0.041) and SIc7a11 (P = 0.029), was elevated in mice fed milk. In 16S rRNA sequencing of gut microbiota, the genus Akkermansia was increased in both the mice fed milk and the FMT group from the mice fed milk. Conclusions The findings of this study suggest that besides increasing the intake of nutrients, such as amino acids, milk consumption also changes the intestinal environment, which might contribute to the mechanism of milk‐induced improvement of sarcopenic obesity.

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Brazilian green propolis improves gut microbiota dysbiosis and protects against sarcopenic obesity

Cited by 21 publications

References 45 publications

Antimicrobial activity of Desplac® oral gel in the subgingival multispecies biofilm formation

Antimicrobial activity of Desplac® oral gel in the subgingival multispecies biofilm formation

Exploring the Prospective Role of Propolis in Modifying Aging Hallmarks

Milk protects against sarcopenic obesity due to increase in the genus Akkermansia in faeces of db/db mice

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