Brassinosteroids alleviate chilling-induced oxidative damage by enhancing antioxidant defense system in suspension cultured cells of Chorispora bungeana

Abstract

Brassinosteroids (BRs), a class of plant steroid hormones, play a significant role in the amelioration of various biotic and abiotic stresses. In order to further explore and elaborate their roles in plants subjected to chilling stress, suspension cultured cells of Chorispora bungeana with or without 24-epibrassinolide (EBR) application were exposed to 4 and 0°C for 5 days. The EBR treated cells exhibited higher viability after exposure to low temperatures compared with the control. Under chilling stress, reactive oxygen species (ROS) levels and lipid peroxidation were increased in the cultured cells, which were significantly inhibited by EBR application. The activities of antioxidative enzymes such as ascorbate peroxidase (APX), catalase (CAT), peroxidase (POD) and superoxide dismutase (SOD) were increased during chilling treatments, and these increases were more significant in the EBR applied suspension cells. The EBR treatment also greatly enhanced contents of ascorbic acid (AsA) and reduced glutathione (GSH) under chilling stress. From these results, it can be concluded that EBR could play the positive roles in the alleviation of oxidative damage caused by ROS overproduction through enhancing antioxidant defense system, resulting in improving the tolerance of C. bungeana suspension cultures to chilling stress.

Response of Salt-Stressed Wheat ( Triticum aestivum L.) to Potassium Humate Treatment and Potassium Silicate Foliar Application

“…Carotenoids are considered as one of the most important non-enzymatic antioxidant which induced decrease the accumulation of ROS under salt stress (Mittler et al, 2004) also, they induce abscisic acid hormone that promotes stomatal closure when exposed to stress (Leung & Giraudat, 1998). More or less similar data were reported by Liu et al (2009).…”

Amelioration of salt-induced oxidative stress in eggplant by application of 24-epibrassinolide

“…Recent developments have shown ⎯⎯⎯⎯ that BRs can enhance plant tolerance to a variety of stresses, such as chilling stress (Liu et al 2009b), salinity (Nunez et al 2003, Ozdemir et al 2004 or pathogen infection (Xia et al 2009). BRs stimulate the antioxidant systems and increase the tolerance of rice to salinity (Nunez et al 2003), tomato seedlings to heat stress (Ogweno et al 2008), and cucumber to osmotic stress (Liu et al 2009a).…”

Alleviating Effects of Exogenous Glutathione, Glycinebetaine, Brassinosteroids and Salicylic Acid on Cadmium Toxicity in Rice Seedlings (Oryza Sativa)

“…In addition, BRs has important function against environmental stress. Now, BRs attracted more attention in adaptive response to abiotic stress, particularly in respect to chilled stress [23], water stress [24], and heat stress [22]. Meanwhile, BRs application can decrease the Cd-induced stress through enhancing antioxidant systems in Brassica juncea [25].…”